The experimental study for the explication of mechanism and therapy on the joint contracture

关节挛缩机制及治疗阐明的实验研究

基本信息

批准号：
12832036
负责人：
OKITA Minoru
金额：
$ 2.3万
依托单位：
NAGASAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12832036/
关键词：
Contracture Soleus muscle Immobilization Muscle fiber Collagen fiber Hyaluronan Passine tension 固定期間伸張運動

项目摘要

Study 1 : The experimental study for the explication of mechanism on the joint contractureImmobilization is often associated with a decrease in muscle elasticity. This condition is called muscle contracture, but the mechanism is not yet clear. We examined changes in ankle joint mobility, passive tension, sarcomere length, collagen fibril arrangement in the endomysium, and hyaluronic acid in muscular tissue 1, 2, 4, 8, and 12 weeks after immobilization of rat soleus muscles in shortened position. Ankle joint mobility decreased with the duration of immobilization. Passive tension on immobilized muscles had increased. Sarcomere length had shortened 1 week after immobilization, but did not show further change 2, 4, 8, and 12 weeks after immobilization. Collagen fibril arrangement in the endomysium 1 and 2 weeks after immobilization was longitudinal to the axis of the muscle fibers, whereas 4, 8, and 12 weeks after immobilization it was circumferential.Hyaluronic acid in muscular tissue had … More increased 1 week after immobilization but remained at the same level at weeks 2, 4, 8, and 12. Histochemically, hyaluronic acid in the endomysium of immobilized muscular tissue was stained more strongly and widely than that in the control tissue. Increased hyaluronic acid in muscular tissue may induce muscle stiffness, but the significance of how hyaluronic acid is related to the mechanism of muscle contracture was not clear. The findings suggest mat muscle contracture started 1 week after immobilization and increased with the length of immobilization. Consequently, muscle contracture is affected by the shortening muscle fibers during the early stage of immobilization, after which the collagen adapts by the fibril arrangement in the endomysium becoming more circumferential. This change in collagen fibril arrangement may cause advanced muscle contracture in the late stage of immobilization.Study 2 : The experimental study for the explication of therapy on the joint contracture1) To determine whether prolonged stretching is preventing for joint contracture in mice. It was found that progression of joint contracture can not be prevented in periods of stretch lasting 10 min, and can prevent in periods of stretch lasting 20 min or more. But the progression of joint contracture can not completely prevented in periods of stretch lasting 30 min.2) To determine whether prolonged stretching is ameliorating for disuse muscle atrophy in immobilized rat soleus muscles. Eighteen male Wistar rats were divided randomly into control (n=3) and immobilization (n=15) groups. The immobilization groups rats were immobilized on ankle joint for 4 weeks, animals were divided into three groups : immobilization alone (group I, n=3), stretch training for 30 min/day for 1or 3 weeks after remobilization (group S, n=6), and spontaneous recovery (non stretch training) for 1 or 3 weeks after remobilization (group NS, n=6). After 1 week of remobilization, muscle fiber diameters for type I fibers in group S had increased significantly compared with group NS. After 3 weeks of remobilization, muscle fiber diameters for types I and II fibers in group S had increased significantly compared with group NS. Our findings suggest that prolonged stretching induces recovery from disuse muscle atrophy after joint contracture. Less

研究1：在关节合同中解释机制的实验研究通常与肌肉弹性的降低有关。这种情况称为肌肉收缩，但机制尚不清楚。我们检查了脚踝关节迁移率，被动张力，肌节长度，内象内胶原原纤维排列以及肌肉组织1、2、4、8和12周的透明质酸的变化。踝关节迁移率随固定持续时间而降低。固定肌肉的被动张力增加了。固定后1周的肌节长度缩短了，但在固定后的2、4、8和12周没有进一步变化。固定后1和2周的胶原原纤维排列纵向与肌肉纤维的轴纵向，而固定固定后的4、8和12周是肌肉组织中的透明型。固定的肌肉组织比对照组织中更强烈，更广泛地染色。肌肉组织中透明质酸的增加可能会诱导肌肉僵硬，但是透明质酸与肌肉收缩机制相关的重要性尚不清楚。发现表明MAT肌肉收缩在固定后1周开始，随着固定的长度而增加。因此，在固定的早期阶段，肌肉收缩会受到缩短的肌肉纤维的影响，此后，胶原蛋白通过内膜中的原纤维排列适应了肌肉纤维。胶原原纤维排列的这种变化可能会在固定的后期引起晚期肌肉缔合。研究2：在关节缔约体上解释治疗的实验研究1）确定长时间的拉伸是否阻止了小鼠的关节缔合。发现在持续10分钟的延伸期间，不能预防关节缔合的进展，并且可以在持续20分钟或更长时间的延伸期内预防。但是，在持续30分钟的拉伸期间，关节缔合的进展无法完全阻止。2）确定长时间的拉伸是否可以改善固定的大鼠比目鱼肌肉中的肌肉萎缩。将18名男性Wistar大鼠随机分为对照（n = 3）和固定（n = 15）组。将大鼠固定组固定在踝关节上4周，将动物分为三组：单独固定（I组，n = 3），伸展训练30分钟/天，持续1或3周后1或3周（S组，n = 6），并在重复恢复后1或3周，在1或3周后进行训练（非伸展训练）。经过1周的重新启动后，与NS组相比，S组I型纤维的肌肉纤维直径显着增加。经过3周的重新启动，与NS相比，S组I型和II型纤维的肌肉纤维直径显着增加。我们的发现表明，长时间的拉伸会导致联合合同后从失去肌肉萎缩中恢复。较少的