Elucidation of symbiotic mechanisms in lichens used secretions from algal partner

利用藻类伙伴的分泌物阐明地衣的共生机制

• 批准号: 12660105
• 负责人: HAMADA Nobuo
• 金额: $ 2.3万
• 依托单位: Osaka City Institute of Public Health and Environmental Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660105/
• 关键词: Lichens; Isolation for Culture; Algal partner; Secondary products; dibenzofurans; 共生関係; 地衣成分

Secondary metabolites in lichen mycobionts serve the key for clarifying the origin of lichen symbiosis. It was thought that the secretion from the algal partner promotes to build up the symbiotic relationship with lichen mycobionts. In this project, isolation and identification of such chemical substances produced by algae was performed, using the technique, which cultures each partner separately. Mycobiont from one strain of Graphis, which produces different substances between isolated stage and symbiotic stage, was used for this project. As the first step, we tried to survey substances from algae which have physiological activity to lichen mycobionts. Substances on Lecanora and Haematomma with similar acitivity was surveyed. Naphthaquinone derivatives were also discovered in mycobionts of Opegrapha and Graphis sp., dibenzofurans in Lecanora and anthraquinones in Haematomma, respectively. In those projects, mixture having activity was detected, but isolation and identification of trigger substance was very difficult because of minor substances. Thus, cultivation of a large amount of colonies is essential. On the other hand, this substance appears to be stable. Now we continue to try purification of this substance still.

地衣真菌生物中的次生代谢物是阐明地衣共生起源的关键。人们认为藻类伙伴的分泌物促进与地衣真菌生物体建立共生关系。在该项目中，使用单独培养每个伙伴的技术对藻类产生的此类化学物质进行了分离和鉴定。该项目使用了一种来自 Graphis 菌株的 Mycobiont，该菌株在分离阶段和共生阶段产生不同的物质。作为第一步，我们尝试调查藻类中对地衣真菌生物具有生理活性的物质。对 Lecanora 和 Haematomma 上具有相似活性的物质进行了调查。在 Opegrapha 和 Graphis sp. 的真菌生物体中也发现了萘醌衍生物，在 Lecanora 中发现了二苯并呋喃，在 Haematomma 中发现了蒽醌。在这些项目中，检测到了具有活性的混合物，但由于物质微量，分离和鉴定触发物质非常困难。因此，培养大量菌落是必要的。另一方面，这种物质似乎很稳定。现在我们还在继续尝试纯化这种物质。

项目成果

N. Hamada, T. Tanahashi, H. Miyagawa and H. Miyawaki: "Characteristics of secondary metabolites from isolated lichen mycobionts"Symbiosis. 31. 23-33 (2001)

N. Hamada、T. Tanahashi、H. Miyakawa 和 H. Miyawaki：“分离的地衣真菌生物次生代谢物的特征”共生。

Moriyasu Y. 他4名: "5-deoxy-7-metylbostry coidin from cultured mycobionts from haematomma sp."Phytochemistry. 58. 239-241 (2001)

Moriyasu Y. 和其他 4 人：“来自血肿菌培养的真菌生物的 5-脱氧-7-甲基博氏菌素”。植物化学 58. 239-241 (2001)

Y.Takenaka 他3名: "Production of xanthone with free radical scavenging Properties, emoclin and seteotionrh by the culture"Z. naturforsch.. 55C. 910-914 (2000)

Y.Takenaka 和其他 3 人：“通过培养物生产具有自由基清除特性的氧杂蒽酮、emoclin 和 seteotionrh”Z. naturforsch.. 910-914 (2000)。

Y.Moriyasu 他4名: "5-doxy-7-methyl Costy coidin from cultured mycobionts from Haematomma sp"Phyto chemistry. 58. 239-241 (2001)

Y. Moriyasu 和其他 4 人：“来自 Haematomma sp 的培养菌体的 5-doxy-7-methyl Costy coidin”植物化学 58. 239-241 (2001)。

T.Amano 他3名: "Production of 5.7-dibydroxy-6-hydromethyl-2-methoxy-1,4-naphoquinove by cultured"Z. naturforsch.. 55b. 6667-6669 (2000)

T. Amano 和其他 3 人：“通过培养生产 5.7-二羟基-6-羟甲基-2-甲氧基-1,4-萘醌” Z. naturforsch.. 55b. 6667-6669 (2000)