DIVERSITY OF THE VALVE ONTOGENY IN DIATOMS AND ITS RELATIONSHIP TO THE PHYLOGENY

硅藻瓣膜个体发育的多样性及其与系统发育的关系

基本信息

批准号：
12640677
负责人：
MAYAMA Shigeki
金额：
$ 2.3万
依托单位：
TOKYO GAKUGEI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Marine and freshwater diatoms were collected from various places in Japan. They were isolated and cultured for the materials of morphological observations and molecular experiments. SEM observations of mature valves revealed some new structures in several species. In Cocconeis the forming valves and the developing perizonium of auxospore. The valve ontogeny of Eunotiashowed some similarity to araphid pennate diatoms in the middle stage of the development. But it showed ontogenetic similarity to biraphid diatoms in the late stages. Use of a fluorescent-label agent, which was taken into the forming valve, could distinguished the newly formed valves after nucleic division and valves already formed before nucleic division. The analyzes of 18S rDNA newly showed the phylogenetic position of Pseudostaurosira, Pseudohimantidium, Berkeleya, Diploneis, Luticola, three Cylindrofheca specis and Nitzschia palea. Close relationship between Pseudohimanthidium and Eunotia, that had been pointed out based on morphology, was not shown in the phylogenetic trees, as Pseudohimantidium formed monophyly with other araphid marine genera. Except for the relationship between Bacillariaceae and Eunotiaceae, which is an opportunist according to the number of OUT and the methods of phylogenetic analyzes, the branching order of the higher rank of diatom taxa reflected that of the ontogenetic similarities from the early to middle stages of the taxa. Sill-like DNA sequences decided in five diatoms showed the high similarity among them, impling its possibility to become a probe for exploring the origin of the biosilica mineralization ability.

海洋和淡水硅藻是从日本各地采集的。对其进行分离培养，作为形态观察和分子实验的材料。对成熟瓣膜的扫描电镜观察揭示了几个物种的一些新结构。在 Coccone 中，有形成瓣膜和发育中的辅助孢子包膜。 Eunotia的瓣膜个体发育在发育中期与羽状硅藻有一定的相似性。但它在晚期表现出与双管硅藻的个体发育相似性。使用荧光标记剂，将荧光标记剂引入形成的瓣膜中，可以区分核酸分裂后新形成的瓣膜和核酸分裂前已形成的瓣膜。 18S rDNA分析新揭示了Pseudostaurosira、Pseudohimantidium、Berkeleya、Diploneis、Luticola、Cylindrofheca spis和Nitzschia papea的系统发育位置。 Pseudohimantidium 和 Eunotia 之间的密切关系，在形态学上已经指出，并没有在系统发育树中显示出来，因为 Pseudohimantidium 与其他蜘蛛纲海洋属形成单系。除了硅藻科和紫杉科的亲缘关系根据OUT的数量和系统发育分析的方法，属于机会主义关系外，硅藻类群高阶的分支顺序反映了硅藻早期到中期个体发育的相似性。类群。 5种硅藻的Sill-like DNA序列显示出它们之间的高度相似性，这意味着它有可能成为探索生物硅矿化能力起源的探针。

项目成果

期刊论文数量（29）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Mayama, S.: "Valuable taxonomic characters in the valve mantle and girdle of some Eunotia species"In : Jahn, R., Kociolek, J.P., Witkowski, A.&P.Compere (eds) Lange-Bertalot-Festschrift. Gantner, Ruggell. 119-130 (2001)

Mayama, S.：“一些 Eunotia 物种的瓣膜地幔和带的有价值的分类学特征”，见：Jahn, R.、Kociolek, J.P.、Witkowski, A.

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鈴木秀和, 南雲保: "神奈川県葉山産の紅藻ピリヒバに着生する珪藻類"日本歯科大学紀要, 一般教育系. 32. 109-118 (2003)

Hidekazu Suzuki、Tamotsu Nagumo：“在神奈川县叶山的红藻 Pirihiba 上生长的硅藻”日本齿科大学通报，普通教育部 32. 109-118 (2003)。

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鈴木秀和,南雲保.: "富山湾深層水で自然繁茂する珪藻."Bull.Toyama Pref.Res.Inst.. No.12. 33-42 (2000)

Hidekazu Suzuki，Tamotsu Nagumo：“富山湾深水中自然生长的硅藻。”Bull.Toyama Pref.Res.Inst. No. 33-42 (2000)。

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Idei, M, Mayama, S.: "Pinnularia acidojaponica M. Idei et H.Kobayasi sp. nov. and P. valdetolerans Mayama et H. Kobayasi sp. nov. -new diatom taxa from Japanese extreme environments"In : Jahn, R., J.P. Kociolek, A. Witkowski & P.Compere (eds) Lange-Bertal

Idei, M, Mayama, S.：“Pinnularia Acidojaponica M. Idei et H.Kobayasi sp. nov. 和 P. valdetolerans Mayama et H. Kobayasi sp. nov. - 来自日本极端环境的新硅藻类群”In：Jahn, R