CONFIGURATION PATTERN OF CT-NUCLEOIDS IN DIATOMS

硅藻中 CT 核苷的构型模式

基本信息

批准号：
06804056
负责人：
MAYAMA Shigeki
金额：
$ 1.41万
依托单位：
TOKYO GAKUGEI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06804056/
关键词：
taxonomy systematics taxonomic characteristics chloroplast ct-nucleoid nucleoid diatom pyrenoid Nitzschia

项目摘要

Configurations of chloroplast-nucleoids (ct-nucleoid) were examined with DAPI-stained diatoms (16 genera, 50 species). The configuration was ring-type in all species except for several Pinnularia and Nitzschia species, in which scattered DAPI-fluorescent globules and short strings of DAPI-fluorescence were observed respectively. As DAPI-fluorescent globules in Pinnularia disappeared by DNase treatment, they were confirmed to contain DNA.These globules were confirmed to be located in the chloroplast by confocal laser microscopy and observations of the sections of the cells embedded in Technovit 7100. These globules corresponded to the discrete areas of thylakoids stack in TEM observation. No membranous structure was detected in these areas. The chloroplasts were isolated and then these globules also isolated. The size of globules were similar to that of each beads in ring-type nucleoid. Employing proteinase K treatment, these globules were lysed and then DNA was diffused. These observat … More ions and experiments prove that the globules scattered throughout the chloroplast are the nucleoids to be a complex of DNA and protein. In Pinnularia, 11 species have the ring-type ct-nucleoid and 9 species have the scattered-type nucleoid as well as the ring-type and 1 species has only the scattered-type. Species with the scattered-type are limited in diatoms of P.sect. Pinnularia and P.sect. Divergentissima. In Nitzschia sigmoidea, we observed that small rectangular pyrenoids were scattered throughout the chloroplasts by propion-carmine staining method. In TEM section, they were lens shape, which was typical in diatoms. Specific localization of Rubisco was detected in this structure using indirect immuno-fluorescence microscopy and electron microscopy with immuno-gold. Although the ring-type nucleoid was not observed in this species, linear DAPI-fluorescence was observed along both sides of the pyrenoids and they seem to be the ct-nucleoids. The configurations of ct-nucleoids in diatoms are diversified as in diatom frustules, and seem to reflect their systematics. Less

用 DAPI 染色的硅藻（16 属，50 种）检查叶绿体核（ct-核）的构型。除了几种羽片藻和菱形藻外，所有物种的构型均为环状，其中散布着 DAPI 荧光球和。分别观察到短串的DAPI-荧光，当DNA酶处理后，羽纹藻中的DAPI-荧光球消失，证实它们存在。通过共聚焦激光显微镜和对嵌入 Technovit 7100 中的细胞切片的观察，证实这些小球位于叶绿体中。这些小球对应于 TEM 观察中类囊体堆叠的离散区域。分离出这些区域的叶绿体，然后也分离出这些小球。小球的大小与环形中的每个珠子的大小相似。采用蛋白酶 K 处理，这些小球被裂解，然后 DNA 被扩散。这些观察离子和实验证明，分散在叶绿体中的小球是 DNA 和蛋白质的复合物。在 Pinnularia 中有 11 个物种。环型ct-核仁，9种具有分散型核仁以及环型，1种仅具有分散型硅藻仅限于 P.sect. 和 P.sect. 在 sigmoidea 中，通过丙酸胭脂红染色法观察到小矩形蛋白核散布在整个叶绿体中。在切片中，它们呈透镜形状，这在硅藻中是典型的，使用间接方法在该结构中检测到了 Rubisco 的特异性定位。免疫荧光显微镜和免疫金电子显微镜虽然在该物种中没有观察到环状核，但沿着蛋白核两侧观察到线性DAPI荧光，它们似乎是ct核的构型。硅藻中的 ct 核仁与硅藻壳中一样多样化，并且似乎较少反映其系统学。