Evolutional study on the endocrine system of brain-pituitary interaction in amphioxus

文昌鱼脑垂体相互作用内分泌系统的进化研究

基本信息

批准号：
12640644
负责人：
KUBOKAWA Kaoru
金额：
$ 2.3万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640644/
关键词：
Amphioxus Pituitary Neural tube EST Hormone Chordata cDNA Reproduction 神経管遺伝子脊索クローニング進化生体調節脳

项目摘要

1. EST analysis of cDNA libraries from Hatschek's pit and neural tube in amphioxus (Branchiostoma belcheri) was attempted in order to the evolutional study on brain-pituitary endocrine system. The results so far are as follows.(1) The successfully removal of he neural tube included brain part enabled the high quality of constructed cDNA library. The EST project of neural tube has been started from 2002 as the collaboration with Profs. K. Agata and S. Kuratani, Riken, Kobe. Nucleotide sequences of the 3800 clones without redundant was analyzed until March 2002. The project continues to obtain all of cDNA clones from amphioxus neural tube. A few hormone genes were obtained such as parathyroid hormone, RFamid, calcitonin, and some kinds of growth factors. In near future, the EST results in amphioxus will reveal an evolution of brain from the neural tube by the comparison of molecule structure and physiological mechanism with specific genes which exist both in amphioxus neural tube and vertebrate brain.(2) The cDNA library from Hatschek's pit includes muscle, skin and nephridium from that the small size of the pit. DNA sequences which might be specific in the pit was Insulin like growth factor binding protein, Insulin like protein, Ferritin, Amyloid, Calcium related proteins. These genes in amphioxus were phylogenetically studied. (3) These results generated from amphioxus contribute the study on mechanism and interactions of neurotransmitters in the brain of vertebrate and mammal.2. Establishment of in situ hybridization in adult amphioxus.3. First success of natural spawning of amphioxus in the laboratory in Japan. Spawning behavior was investigated by movie during reproductive period, two months, and determined the spawning conditions and estimated behavioral and environmental factors for success of spawning.

1。试图对大脑 - 垂体内分泌系统的进化研究进行了hatchek坑和神经管（Branchiostoma belcheri）中的cDNA库的分析。到目前为止的结果如下。（1）成功去除HE神经管的脑部包括大脑部分，使构造的cDNA库的高质量。神经管的EST项目从2002年开始，作为与教授的合作。 K. Agata和S. Kuratani，Riken，Kobe。直到2002年3月，分析了3800克隆的核苷酸序列。该项目继续从两栖神经管中获取所有cDNA克隆。获得了一些激素基因，例如甲状旁腺激素，RFAMID，降钙素和某些生长因子。在不久的将来，通过将分子结构和生理机制与特定基因的比较，在两栖神经管和脊椎动物大脑中存在的特定基因的比较，将揭示大脑从神经管中的演变。在凹坑中可能特异的DNA序列是胰岛素，例如生长因子结合蛋白，胰岛素，例如蛋白，铁蛋白，淀粉样蛋白，钙相关蛋白。对两性基因的这些基因进行了系统发育研究。（3）这些结果是由两栖焦油产生的，对脊椎动物和哺乳动物大脑中神经递质的机制和相互作用进行了研究。2。建立成人两栖动物的原位杂交3。日本实验室中两栖产卵自然产卵的首要成功。在生殖时期，两个月的电影中通过电影研究产卵行为，并确定了产卵成功的产卵条件以及估计的行为和环境因素。