Analysis of the transport of generative cells in pollen tubes of higher plants

高等植物花粉管中生殖细胞运输的分析

• 批准号: 12640636
• 负责人: YOKOTA Etsuo
• 金额: $ 3.07万
• 依托单位: Himeji Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640636/
• 关键词: Pollen tube; Generative cell; Myosin; Actin; Cytoplasmic streaming; Lily; 微小管; 花粉管核

It has been suggested that actin cytoskeleton is involved in the transport of generative cells In pollen tubes of higher plants. We studies the involvement of myosin in this transport and tried to identify the myosin from lily pollen tubes1. Involvement of myosin in the transport of generative cells. 2,3-butanedione monoxime (BDM), a myosin inhibitor, reversibly suppressed the cytoplasmic streaming and the transport of both generative cells and vegetative nuclei in lily pollen tubes. The length and morphology of generative cells and vegetative nuclei were altered by the treatment with BDM. The organization of actin-filaments in the treated pollen tubes with BDM was only slightly perturbed, suggesting that myosin is actually responsible for the generation of motive force for the transport of generative cells.2. Identification of myosin responsible for the transport of generative cells A myosin composed of 120-kDa heavy chain was isolated from lily pollen tubes. An antigenicity of this 120-kDa heavy chain was different from the 170-kDa myosin heavy chain which is involved in the cytoplasmic streaming in higher plant cells. It was considered that this myosin is a candidate of molecular motor for the transport of generative cells or vegetative nuclei.

有人提出，肌动蛋白细胞骨架参与高等植物花粉管中生殖细胞的运输。我们研究了肌球蛋白在这种运输中的参与，并试图从百合花粉管中识别肌球蛋白1。肌球蛋白参与生殖细胞的运输。 2,3-丁二酮单肟 (BDM) 是一种肌球蛋白抑制剂，可逆地抑制百合花粉管中的细胞质流动以及生殖细胞和营养细胞核的运输。 BDM 处理改变了生殖细胞和营养细胞核的长度和形态。经过BDM处理的花粉管中肌动蛋白丝的组织仅受到轻微干扰，这表明肌球蛋白实际上负责产生生殖细胞运输的动力。2．负责生殖细胞运输的肌球蛋白的鉴定从百合花粉管中分离出由120-kDa重链组成的肌球蛋白。该120-kDa重链的抗原性不同于参与高等植物细胞中的细胞质流动的170-kDa肌球蛋白重链。人们认为这种肌球蛋白是运输生殖细胞或营养细胞核的分子马达的候选者。

项目成果

Teruo Shimmen: "Plant myosins"Protoplasma. 214. 1-10 (2000)

Teruo Shimmen：“植物肌球蛋白”原生质体。

Etsuo Yokota: "Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A"Protoplasma. 213. 184-193 (2000)

Etsuo Yokota：“肌动蛋白细胞骨架负责由蛋白磷酸酶抑制剂花萼蛋白 A 诱导的根毛细胞中细胞质组织的变化”原生质。

Motoki Tominaga: "Mechanism of inhibition of cytoplasmic streaming by a myosin inhibitor 2,3-butanedione monoxime"Protoplasma. 213. 46-54 (2000)

Motoki Tominaga：“肌球蛋白抑制剂 2,3-丁二酮单肟抑制细胞质流动的机制”原生质体。

Etsuo Yokota: "Identification and characterization of higher plant myosins responsible for cytoplasmic streaming"Journal of Plant Research. 113. 511-519 (2000)

Etsuo Yokota：“负责细胞质流的高等植物肌球蛋白的鉴定和表征”植物研究杂志。

Takeshi Sakamoto: "Direct observation of processive movement by individual myosin V molecules"Biochemical and Biophysical Research Communications. 272. 586-590 (2000)

Takeshi Sakamoto：“直接观察单个肌球蛋白 V 分子的持续运动”生物化学和生物物理研究通讯。