AMPA receptor and its role in astroglia

AMPA受体及其在星形胶质细胞中的作用

• 批准号: 11680815
• 负责人: SEKIGUCHI Masayuki
• 金额: $ 2.24万
• 依托单位: National Center of Neurology and Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680815/
• 关键词: Astroglia; AMPA receptor; PEPA; Cyclothiaizde; GDNF; Cyclthiazide; 脱感作; カルシウム

We have prepared astroglia-rich cultures from the embryonic rat cerebral cortex, and investigated activation of AMPA receptor in astroglia using calcium-imaging. In our culture condition, AMPA receptor-evoked increases in intracellular calcium concentration was observed in a small population of cells. This calcium responses were potentiated by PEPA and cyclothiazide, allosteric potentiators of AMPA receptors, and the extent of potentiation by two drugs depends upon cells. This is probably due to cell-dependent heterogeneity of AMPA receptors. In these cells, membrane currents mediated by AMPA receptors were recorded using patch clamp recordings.The current responses were also potentiated by PEPA, and show inwardly rectifying I-V relation, at least in the cells we tested. We have examined gene expression stimulated by the activation of glial AMPA receptors. For this purpose, quantitative RT-PCR was carried out for mRNA from glial cultures. We could not detect significant increases in expression of GDNF mRNA level in our cultures. We have further investigated on other gene, and prepared glial cells from other brain regions, in order to get further information on glial AMPA receptors.

我们已经从胚胎大鼠大脑皮层制备了富含星形胶质细胞的培养物，并使用钙成像研究了AMPA受体在星形胶质细胞中的激活。在我们的培养条件下，在少数细胞群中观察到了AMPA受体引起的细胞内钙浓度的增加。 PEPA和环硫嗪，AMPA受体的变构增强剂和两种药物的增强程度取决于细胞，从而增强了这种钙反应。这可能是由于AMPA受体的细胞依赖性异质性。在这些细胞中，使用斑块夹记录记录了由AMPA受体介导的膜电流。当前的响应还通过PEPA增强，并至少在我们测试的细胞中显示了内部整流的I-V关系。我们已经检查了通过神经胶质AMPA受体激活刺激的基因表达。为此，对神经胶质培养物进行了定量RT-PCR。我们无法检测到我们培养物中GDNF mRNA水平表达的显着增加。我们进一步研究了其他基因，并从其他大脑区域制备了神经胶质细胞，以获取有关神经胶质AMPA受体的更多信息。

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