Asymmetric co-localization of Flamingo, a seven-pass transmembrane cadherin, and Dishevelled in planar cell polarization.

Flamingo（一种七次跨膜钙粘蛋白）的不对称共定位，以及平面细胞极化中的 Disheveled。

• 批准号: 11680721
• 负责人: UEMURA Tadashi
• 金额: $ 2.3万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680721/
• 关键词: planar cell polarity; Wnt; Frizzled; Dishevelled; Flamingo; Drosophila; planar polarity; カドヘリンスーパーファミリー

The Drosophila wing provides an appropriate model system to study genetic programming of planar cell polarity (PCP). Each wing cell respects the proximodistal (PD) axis ; i.e., it localizes an assembly of actin bundles to its distalmost vertex, producing a single prehair. This PD polarization requires re-distribution of Flamingo (Fmi), a seven-pass transmembrane cadherin, to proximal/distal cell boundaries ; otherwise the cell mislocalizes the prehair. Achievement of the biased Fmi pattern depends on two upstream components in the PCP signaling pathway : the Frizzled (Fz) receptor for a hypothetical polarity signal and the intracellular protein Dishevelled (Dsh). Here we visualized endogenous Dsh in the developing wing. A portion of Dsh co-localized with Fmi, and the distributions of both proteins were interdependent. Furthermore Fz controlled the association of Dsh with cell boundaries, which association was correlated with the presence of hyperphosphorylated forms of Dsh. Our results, together with a recent study on Fz distribution, support the possibility that Fz, Dsh, and Fmi constitute a signaling complex and that its restricted localization directs cytoskeletal reorganization only at the distal cell edge.

果蝇翅膀提供了一个合适的模型系统来研究平面细胞极性（PCP）的遗传编程。每个翼细胞都遵循近远端 (PD) 轴；即，它将肌动蛋白束的集合定位到其最远端的顶点，产生单个前毛。这种 PD 极化需要 Flamingo (Fmi)（一种七次跨膜钙粘蛋白）重新分布到近端/远端细胞边界；否则细胞会错误定位前毛。偏向 Fmi 模式的实现取决于 PCP 信号通路中的两个上游组件：假设极性信号的卷曲 (Fz) 受体和细胞内蛋白散乱 (Dsh)。在这里，我们对发育中的机翼中的内源性 Dsh 进行了可视化。 Dsh 的一部分与 Fmi 共定位，并且两种蛋白的分布是相互依赖的。此外，Fz 控制 Dsh 与细胞边界的关联，这种关联与 Dsh 过度磷酸化形式的存在相关。我们的结果以及最近对 Fz 分布的研究，支持 Fz、Dsh 和 Fmi 构成信号复合物的可能性，并且其受限定位仅在远端细胞边缘指导细胞骨架重组。

项目成果

Liu 他4名: "Flamingo controls the planar polarity of sensory bristles and asymmetric division of sensory organ precursors in Drosophila"Current Biology. 9. 1247-1250 (1999)

Liu 等人 4：“火烈鸟控制果蝇感觉刚毛的平面极性和感觉器官前体的不对称分裂”《当代生物学》9. 1247-1250 (1999)

碓井理天,上村匡: "平面内細胞極性形成の分子機構Molecular Mechanism of Planar Cell Polarity Formation"細胞工学. 19. 1627-1633 (2000)

Riten Usui，Tadashi Uemura：“平面细胞极性形成的分子机制”细胞工程 19. 1627-1633 (2000)。

Takeichi, M: "Patterning of cell assemblies regulated by adhesion receptors of the cadherin superfamily."Phil.Trans.R.Soc.Lond.B. 355. 885-890 (2000)

Takeichi, M：“钙粘蛋白超家族粘附受体调节细胞组装的模式。”Phil.Trans.R.Soc.Lond.B.

碓井理夫,上村匡: "平面内細胞極性形成の分子機構Molecular Mechanism of planar Cell Polarity Formation."細胞工学. 19. 1627-1633 (2000)

Rio Usui，Tadashi Uemura：“平面细胞极性形成的分子机制。”细胞工程。19。1627-1633（2000）

Usui 他7名: "Flamingo, a seven-pass transmembrance cadherin, regulates planar cell polarity under the control of Frizzled"Cell. 98. 585-595 (1999)

Usui 和其他 7 人：“Flamingo，一种七次跨膜钙粘蛋白，在 Frizzled 的控制下调节平面细胞极性”Cell 98. 585-595 (1999)。