Characterization of cultured synovial cells from human TMJ

人颞下颌关节培养滑膜细胞的表征

• 批准号: 11672017
• 负责人: OGURA Naomi
• 金额: $ 2.37万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672017/
• 关键词: Cultured synovial cells; TMJ; IL-1β; TNF-α; Plasminogen activator; IL-6; IL-8; MCP-1; 顎関節症; ヒト顎関節滑膜細胞

The aims of the present study were, firstly, to isolate and to characterize culture synovial cells obtained from human TMJ specimens, and secondly, to investigate the productions of cytokines and enzymes in those cells.<Methods> Synovial cells were isolated from TMJ synovial tissue using an out growth method and then cultured in Ham F12 containing 20% or 10% fetal bovine serum. Synovial cells were examined cell-specific markers using immunocytochemistry. In the next, synovial cells were treated with IL-1β or TNF-α, which was increased in synovial fluids from patients with TMJ disorders. Activity of plasminogen activator (PA), which is associated with extracellular matrix degradation, was measured by enzyme assay. Amounts of IL-6, IL-8 and macrophage attractant protein (MCP)-1 were measured by ELISA. Gene expressions of these cytokines were also investigated using RTPCR method.<Rersults> Synovial cells were positive for fibroblast-specific marker and vimentin. In contrast, the cells were negative for macrophage marker, HLA class II and dendritic cell marker. Then, synovial cells were treated with or without IL-1β. IL-1β increased IL-6, IL-8 and MCP-1 production in synovial cells in a time- and dose-dependent manner. IL-1β stimulated gene expression of these cytokines in them. IL-1β also enhanced PA activity in synovial cells. In the next, the cells were treated with TNF-α. TNF-α enhanced productions and gene expressions of IL-8 and MCP-1.<Conclusions> We found that cultured synovial cells might provide important advantages for studies of the cellular and molecular mechanisms in TMJ. In addition, PA activity and IL-6, IL-8, and MCP-1 productions may be increased in synovial cells when IL-1β and TNF-α levels are increased in synovial fluids from patients with TMJ disorders.

目前的统计目的是分离并表征从人TMJ标本获得的培养细胞，以估计使用20％或10％胎儿的HAM F12中的细胞中细胞因子和酶的生产牛血清。在下一个中，滑膜细胞用IL-1β或TNF-β进行了治疗，从而通过IL-6，IL-6，IL-6，IL-6，IL-6，IL-6，IL-8，从而测量TMJ基质降解患者的滑液。巨噬细胞蛋白质（MCP）-1通过ELISA测量。 IL-1β以iL-1的速度增加了IL-8的iL-8。 IL-8和MCP-1的基因表达式。当IL-1β和TNF-α水平升高的滑液中时，滑膜细胞的流液中，来自TMJ疾病患者的滑液。

项目成果

Naomi Ogura: "Interleukin-1β induces interleukin-6 mRNA expression and protein production in synovial cells from human temporomandibular joint"Journal of Oral Pathology and Mecine. (in press).

Naomi Ogura：“Interleukin-1β 诱导人颞下颌关节滑液细胞中 interleukin-6 mRNA 表达和蛋白质产生”《口腔病理学与医学杂志》（正在出版）。

Makiko Tobe: "Effect of IL-1β on plasminogen activator activity in human synovial cells from TMJ"Medicine and Biology. 143. 139-143 (2001)

Makiko Tobe：“IL-1β 对 TMJ 人类滑膜细胞纤溶酶原激活剂活性的影响”医学与生物学 143. 139-143 (2001)

戸邊真希子: "ヒト顎関節滑膜細胞Plasminogen activator活性へ与えるIL-1βの影響"医学と生物学. 143. 139-143 (2001)

Makiko Tobe：“IL-1β 对人颞下颌关节滑膜细胞纤溶酶原激活剂活性的影响”医学与生物学 143. 139-143 (2001)

Naomi Ogura: "Interleukin -1β induces interleukin-6 mRNA expression and protein production in synovial cells from human temporomandibular joint"Journal of Oral Pathology and Medicine. (in press).

Naomi Ogura：“白细胞介素 -1β 诱导人颞下颌关节滑液细胞中白细胞介素 6 的 mRNA 表达和蛋白质产生”《口腔病理学与医学杂志》（正在出版）。

N.Ogura, M.Tobe, H.Sakamaki, H.Kujiraoka, M.Akiba, Y.Abiko, H.Nagura: "Interleukin-1β induces interleukin-6 mRNA expression and protein production in synovial cells from human temporomandibular joint"Journal of Oral Pathology and Medicine. (in press).

N.Ogura、M.Tobe、H.Sakamaki、H.Kujiraoka、M.Akiba、Y.Abiko、H.Nagura：“Interleukin-1β 诱导人颞下颌关节滑膜细胞中 IL-6 mRNA 表达和蛋白质产生”杂志口腔病理学和医学（正在出版）。