Defense mechanism of the junctional epithelium in the of periodontal tissue by the adhesive molecule

黏附分子对牙周组织连接上皮的防御机制

• 批准号: 11671815
• 负责人: HASHIMOTO Sadamitsu
• 金额: $ 2.18万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671815/
• 关键词: iunctional epithelium; epithelial attachment; inner basal Iamina; outer basal Iamina; adhesive molecule; extracellular matrix; Laminin-5; Integrin-α&β; 半接着斑; ラミニン; ラミニン5; インテグリン

During the progress of periodontal disease, the epithelial attachment destruction or detachment from enamel surface is necessary at the initial stage of marginal peridontitis. In the meantime, the newly formed epithelial attachment to the tooth surface with regencrative junctional epithelium becomes an indispensable factor in during the healing stage of periodonatal disease. For understanding the perriodontal disease, it is important to elucidate the mechanism of epithelial attachment to the enamel surface and exposed root surface with adhesive molecule and extracellular matrix.The purpose of this study is to clarify, the expression of adhesive molecule and the ligand extracellular matrix in the normal rat junctiona epithlium and the regenerative junctional epithelium. In the normal rat junctional epithelium, Laminin-5 positive reaction was recognized at the exclusively inner basal lamina located in enamel surface side as clear thin line by conventional immunofluorescent staining. Inte … More grin-α6β4 is one of the adhesion protein on the cell membrane and component of the half desmosome. Laminin-5 is the ligand of Integrin-α6β4. Integrin-α6 β4 also located in similarly existing in the inner basal lamina located in enamel surfaco side and also, in the outer basal lamina located in connective tissue surface sire. Immunoelectron microscopically, using the high resolution immuno colloidal gold labelling method by ultra thin frozen section methods, 10nm colloidal gold particle which indicated Laminin-5 localization were exclusivoly located in the inner basal lamina. These gold particles were lined correspond to the lamina densa of the inner basal lamina. On the other hand, only few gold particles same as background level were recognized at the outer basal lamina of junctional epithelium. These results strongly suggested that, in the junctional epithelium, Laminin-5 expression is difrerence between the inner and outer basal lamina, and Laminin-5 secretion of the most inner side of the junctional epithelial cells was key of the epithelial attachment. Father study in detail on Laminin-5 sub units, α3-、β3-、y2 chain have to examine in future. Less

在牙周疾病的进展过程中，在边际围膜炎的初始阶段必须使用上皮附着的破坏或脱离牙釉质表面。同时，在牙周疾病的愈合阶段，新形成的上皮附着在牙齿表面上是必不可少的因素。为了理解牙齿牙齿疾病，重要的是要阐明上皮附着在牙釉质表面上的机制，并用粘附分子和细胞外基质暴露于牙根表面。这项研究的目的是阐明，粘合分子的表达是粘合分子的表达，而粘合剂分子的表达是正常的regithel eNithel Epithita和Regithel Epithital Epithital and regithel and regithel and regithel and regithel and regithel and regithel。在正常的大鼠连接性上皮中，层粘连蛋白5的阳性反应在位于搪瓷表面侧的唯一内部碱性层中被识别为常规免疫荧光染色的透明细线。 Inte…更多的grin-α6β4是细胞膜和半腔体成分上的粘合剂蛋白之一。层粘连蛋白5是整联蛋白-α6β4的配体。整联蛋白-α6β4也位于位于搪瓷表面侧的内部基本层中，以及位于结缔组织表面父亲的外部基本层中。通过超薄的胶体截面方法，使用高分辨率免疫胶体金标记方法进行免疫电子显微镜，10nm胶体金颗粒，表明层粘连蛋白5定位专门位于内部基本层层中。这些金颗粒被衬里对应于内部碱性薄片的层。另一方面，在连接上皮的外部基本层层中仅识别出与背景水平相同的少数金颗粒。这些结果强烈表明，在连接性上皮中，层粘连蛋白5的表达是内部和外部层之间的不同，而粘胺-5分泌的层粘连蛋白5分泌是连接性上皮细胞的最内侧，是上皮附着的关键。父亲对层粘连蛋白5子单元进行详细研究，α3-，β3-，Y2链将来必须检查。较少的

项目成果

Kaneko, H., Hashimoto, S., Enokiya, Y., Ogiuchi, H., Shimono, M.: "Cell proliferation and death of hertwig's epithelial root sheath in the rat."Cell Tissue Research. 298(1). 95-104 (1999)

Kaneko, H.、Hashimoto, S.、Enokiya, Y.、Ogiuchi, H.、Shimono, M.：“大鼠赫特维希上皮根鞘的细胞增殖和死亡。”细胞组织研究。

Ikeda, H., Yamada, T., Yoshinari, M., Ohsaki, Y., Ayukawa, Y., Kido, M., Inoue, T., Shimono, M., Koyanoa, K., Tanaka, T.: "Ultrastructural and immunoelectron microscopic studies of the peri-implant epithelium-implant (Ti-6Al 4V) interface of rat maxilla."

池田 H.、山田 T.、吉成 M.、大崎 Y.、鲇川 Y.、木户 M.、井上 T.、下野 M.、小矢野 K.、田中 T.：

Ikeda, H., et al.: "Ultrastructural and immunoelectron microscopic studies of the peri-implant epithelium-implant (Ti-bAl 4V) interface of rat maxilla"J.Priodontol. 71. 961-973 (2000)

Ikeda, H. 等人：“大鼠上颌骨种植体周围上皮-种植体 (Ti-bAl 4V) 界面的超微结构和免疫电子显微镜研究”J.Priodontol。

Kaneko, H., et al.: "Cell proliferation and death of hertwig's epithelial root sheath in the rat"Cell Tissue research. 298. 95-104 (1999)

Kaneko, H. 等人：“大鼠 Hertwig 上皮根鞘的细胞增殖和死亡”细胞组织研究。

Shimono, M., et al.: "Regulatory mechanisms of periodontal regeneration.In "Th biology of tooth regeneration""Microscopy research and technique.. (in press). (2002)

Shimono, M., et al.：“牙周再生的调节机制。在“牙齿再生的生物学”中”显微镜研究和技术..（出版中）。