HOMESTASIS OF THE PERIODONTAL LIGAMENT

牙周韧带的稳态

• 批准号: 08672094
• 负责人: HASHIMOTO Sadamitsu
• 金额: $ 1.66万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672094/
• 关键词: periodontal ligament; Malassez epithelial rests; Hertwig's epithelial sheath; junctional epithelium; immunohistochemistry; Immunofluorescence; homeostasis; laser scanning microscope

The periodontal ligament is located between cementum and alveolar bone and the width of the periodontal ligament is kept in the various condition such as aging, vertical or horizontal orthodontic tooth movement without ankylosis. The purpose of this study was to elucidate the role of Hertwig's epithelial root sheath, Malassez epithelial rests cell and fibroblasts in the homeostasis of periodontal ligament. Cytokeratin, type IV Collagen, Laminin, Connexin 43, 32, Actin immunolocalization was examined by conventional Immunofluorescence, immunohistochemistry and laser scanning microscope in the 1 to 12 weeks S.D.rat periodontal tissue, cultured pig Malassez epithelial rests cells and experimental chronic periodontitis in S.D.rat. Cytokeratin-19 and Connexin 43 were located in the Malassez epithelial rests. Laminin was located in the basement membrane surrounding the Hertwig's epithelial root sheath, Malassez epithelial rests cell, and positive reaction was reduced and disappear in the pulp side of the Hertwigts epithelial root sheath during odontoblasto developing and dentinogenesis. Proliferative activity of the junctional epithelium, long junctional epitheliun-i, fibroblasts of the periodontal ligament were examined using argyrophilic nucleolar organizer region (AgNORs) staining and PCNA immunohistochemical staining. Proliferative cells were recognized at the connective tissue side in the both junctional and long junctional epithelium.

牙周韧带位于牙骨质和肺泡骨之间，牙周韧带的宽度保持在各种状态，例如衰老，垂直或水平正畸牙齿运动，而无需坚硬。这项研究的目的是阐明赫特维格的上皮根护套，马拉西斯上皮静止的细胞和成纤维细胞在牙周韧带的稳态中。细胞角蛋白，IV型胶原蛋白，层粘连蛋白，连接蛋白43、32，肌动蛋白免疫定位通过常规免疫荧光，免疫组织化学和激光扫描显微镜在1至12周的S.D.Rat量化组织中，培养的PigMalassez Eptherel Restins Chronic secroneds secronef s. s.d.rat量化。细胞角蛋白19和连接蛋白43位于马拉塞斯上皮静止。层粘连蛋白位于Hertwig上皮根护套周围的地下膜中，Malassez上皮静止，阳性反应降低并在Odontoblasto发育和牙本质发生过程中Hertwigts上皮根鞘的果肉侧消失。连接性上皮，长连接性上皮1，牙周韧带的成纤维细胞的增殖活性，使用芳基核酸核组织者区域（AGNORS）染色和PCNA免疫组织化学染色检查了牙周韧带的成纤维细胞。在连接组织的结缔组织和长连接上皮的结缔组织中识别出增殖细胞。

项目成果

Shimono, M., Hashimoto, S.et.al.: "Homestasis and regeneration of the periodontal ligament." Oral Med.& Pathol. 3. 1-12 (1998)

Shimono, M., Hashimoto, S.et.al.：“牙周膜的稳态和再生。”

Uno T,Hashimoto S,et.al.: "A study of the proliferative activity of the long junctional epithelium using argyrophilic nucleolar organizer region (AgNORs) staining." J.Periodontal Res. 33. 298-309 (1998)

Uno T、Hashimoto S 等人：“使用嗜银核仁组织区 (AgNOR) 染色研究长连接上皮的增殖活性。”

Uno T, Hashimoto S, et.al.: "A study of the proliferative activity of the long junctional epithelium using argyrophilic nucleolar organizer region (AgNORs) staining." J.Periodontal Res,. 33. 298-309 (1998)

Uno T、Hashimoto S 等人：“使用嗜银核仁组织区 (AgNOR) 染色研究长连接上皮的增殖活性。”

Shimono, M., Hashimoto, S.et.al.: "Homestasis and regeneration of the periodontal ligament." Oral Med.& Pathol.3. 1-12 (1998)

Shimono, M., Hashimoto, S.et.al.：“牙周膜的稳态和再生。”

Inoue, T.et.al.: "Immunolocalization of proliferating cell nuclear antigen in the peri-implant epithelium." Bull.Tokyo.Dent.Coll.38. 187-193 (1997)

Inoue, T.et.al.：“种植体周围上皮中增殖细胞核抗原的免疫定位。”