Role of osteopontin in the calcification of chronic otitis media

骨桥蛋白在慢性中耳炎钙化中的作用

• 批准号: 11671714
• 负责人: SAKAGAMI Masafumi
• 金额: $ 2.18万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671714/
• 关键词: chronic otitis media; calcification; tympanosclerosis; osteopontin; macrophage; tymapanic membrane; tympanic mucosa; in situ hybridization

Tympanosclerosis is a condition leading to a calcification process in the middle ear, and often develops after chronic inflammation of the middle ear. Since osteopontin (OPN) has been shown to participate in the pathological calcification, we here investigated whether OPN is involved in the process of calcification in tympanosclerosis. The tympanic membrane and middle ear mucosa, obtained from patients of tympanosclerosis and chronic otitis media, were histologically classified depending on the calcification degree. In hyalinized tissues with macroscopic calcification and fibrous tissues with microscopic calcification, OPN was also found in the calcifying foci, and many OPN mRNA-expressing cells, determined by in situ hybridization, located around their foci. Moreover, immunohistochemical double staining of OPN and CD68 showed that the OPN-expressing cells were CD68-positive indicating these cells were macrophages. In inflammatory tissues without calcification, immunohistochemistry of CD68 and in situ hybridization of OPN mRNA revealed that most OPN mRNA-expressing cells were CD68-positive. The expression of OPN mRNA in inflammatory tissues was also shown by reverse transcriptase polymerase chain reaction. These results suggested that OPN secreted by exudate macrophages might be an important regulator in the calcification of tympanosclerosis.

鼓室硬化症是一种导致中耳钙化过程的疾病，通常在中耳慢性炎症后发生。由于骨桥蛋白（OPN）已被证明参与病理性钙化，我们在此研究OPN是否参与鼓室硬化的钙化过程。取自鼓室硬化和慢性中耳炎患者的鼓膜和中耳粘膜，根据钙化程度进行组织学分类。在具有宏观钙化的透明组织和具有微观钙化的纤维组织中，在钙化灶中也发现了OPN，并且通过原位杂交测定，许多表达OPN mRNA的细胞位于其灶周围。此外，OPN和CD68的免疫组织化学双染色显示表达OPN的细胞是CD68阳性，表明这些细胞是巨噬细胞。在没有钙化的炎症组织中，CD68的免疫组化和OPN mRNA的原位杂交显示大多数OPN mRNA表达细胞为CD68阳性。逆转录聚合酶链反应也显示了炎症组织中 OPN mRNA 的表达。这些结果表明，渗出巨噬细胞分泌的 OPN 可能是鼓室硬化钙化的重要调节因子。

项目成果

Sakagami M: "Role of osteopontin in the rodent inner ear as revealed by in situ hybridization."Med Electron Microsc. 33. 3-10 (2000)

Sakagami M：“通过原位杂交揭示了骨桥蛋白在啮齿动物内耳中的作用。”Med Electron Microsc。

Makiishi-Shimobayashi C,Sakagami M, et al.: "Expression of osteopontin by exudate macrophages in inflammatory tissues of the middle ear."Hearing Research. (in press). (2001)

Makiishi-Shimobayashi C、Sakagami M 等人：“中耳炎症组织中渗出巨噬细胞表达骨桥蛋白。”听力研究。

Makiishi-Shimobayashi C,Sakagami M, et al.: Expression of osteopontin by exudate macrophages in inflammatory tissues of the middle ear. (in press). (2001)

Makiishi-Shimobayashi C，Sakagami M，等人：中耳炎症组织中渗出巨噬细胞表达骨桥蛋白。

Sakagami M: "Role of osteopontin in the ordent inner ear as revealed by in situ hybridization"Medical Electron Microscopy. 33. 3-10 (2000)

Sakagami M：“通过原位杂交揭示骨桥蛋白在定向内耳中的作用”医学电子显微镜。