The Study on Cytokine Production of Helper T cell and its Therapeutic Effect in Allergic Rhinitis

辅助性T细胞产生细胞因子及其治疗变应性鼻炎的研究

• 批准号: 11671693
• 负责人: KURONO Yuichi
• 金额: $ 0.77万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671693/
• 关键词: nasal allergy; helper T cell; Th2; Th1; cytokine profile; therapeutic effect; ヘルパーT細胞

By use of flow cytometry, we detected the intracellular cytokine of T cells, IL-4 and TNF-α, in peripheral blood and palatine tonsil from the patients with allergic rhinitis. In addition, we investigated the therapeutic effect to the rate of Th2/Th1 using anti-allergic agents, suplatast tosilat The suplatast tosilate is known that the agents which will normalize the Th2/Th1 unbalance.The results showed that the rate of Th2/Th1 from the patients with nasal allergy was higher than that from healthy control in peripheral mononuclear cells and tonsillar mononuclear cells, but there was no statistical significant.There was no definite therapeutic effect on the rate of Th2/Th1 in the peripheral mononuclear cells from the patients with nasal allergy after short-term using suplatast tosilate. But some cases who were clinically effective to the suplatast tosilate the rate of Th2/Th1 was reduced after using of suplatast tosilate.

采用流式细胞术检测变应性鼻炎患者外周血和腭扁桃体中T细胞细胞内细胞因子IL-4和TNF-α，并考察其对Th2/率的治疗效果。 Th1使用抗过敏剂，suplatast tosilat 已知suplatast tosilat可以使Th2/Th1不平衡正常化。结果显示，Th2/Th1的比率从鼻过敏患者外周血单个核细胞、扁桃体单个核细胞较健康对照升高，但无统计学意义。鼻过敏患者外周血单个核细胞Th2/Th1率无明确治疗效果。短期使用甲苯磺酸舒拉司特后出现过敏，但部分临床上对甲苯磺酸舒拉司特有效的病例，使用甲苯磺酸舒拉司特后Th2/Th1率降低。

项目成果

黒野祐一: "中耳炎とサイトカイン"耳鼻臨床. 93(4). 259-265 (2000)

Yuichi Kurono：“中耳炎和细胞因子”Otorhinolaryngology 93（4）（2000）。

黒野祐一 他: "上気道感染症と粘膜免疫"日本耳鼻咽喉科感染症研究会会誌. 17(1). 176-179 (1999)

Yuichi Kurono 等人：“上呼吸道感染和粘膜免疫”日本耳鼻喉传染病研究组杂志 17（1）176-179（1999）。

黒野祐一 他: "エアロゾルによる上気道ワクチン療法の可能性"耳展. 43(補1). 37-42 (2000)

Yuichi Kurono 等人：“基于气雾剂的上呼吸道疫苗治疗的可能性”Mimiten 43（补充1）（2000 年）。

Shoji Matsune: "Application of YAMIK sinus catheter for Patients with paranasal Sinusitis with and without nasal allergy"Auris Nasus larynx. 27. 343-347 (2000)

Shoji Matsune：“YAMIK鼻窦导管在伴有和不伴有鼻过敏的鼻旁窦炎患者中的应用”Auris Nasus喉。

T.Miyanohara et al.: "Effects of clarithromycin in cultured human nasal epithelial cells and fibroblasts."Laryngoscope . 110. 126-131 (2000)

T.Miyanohara 等人：“克拉霉素对培养的人鼻上皮细胞和成纤维细胞的影响。”喉镜。