Immunological Treatment against Upper Respiratory Tract Infection caused by Haemophilus influenzae ; The Roleof Mucosal Immunity

流感嗜血杆菌引起的上呼吸道感染的免疫治疗；

基本信息

批准号：
06671724
负责人：
KURONO Yuichi
金额：
$ 1.15万
依托单位：
Otia Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671724/
关键词：
Haemophilus influenzae Mucosal immunity Upper respiratory tract infection Otitis media Nasopharynx Bacterial adherence Outer membrane protein Oral vaccination

项目摘要

1)Detection of Haemophilus inflenzae by PCR :PCR was applied to detect H.influenzae from middle ear effusion of patients with otitis media with effusion. The incidence of P6-DNA was significantly higher than that of bacteria detected by conventional culture method. P6-DNA was also frequently found in adenoids. The results suggest that presence of H.influenzae in middle ear effusion and in the nasopharynx is more common than previously reported.2)Mucosal immune responses in the nasopharynx and adenoids :Secretory IgA antibody activities in nasopharyngeal secretions of patients with otitis media with effusion were determined by ELISA.The antibody titers against H.influenzae were inversely correlated with the number of H.influenzae in the nasopharynx. ELISPOT assay of adenoid lymphocytes showed the presence of IgA and IgG antibody secreting cells specific to outer membrane proteins of H.influenzae. The number of lymphocytes were inversely correlated with the number of H.influenzae in aden … More oids. The result suggest that nasopharyngeal colonization of H.influenzae might be inhibited by secretory IgA in the nasopharynx, and that adenoids might regulate mucosal immune responses in the nasopharynx.3)Effects oral immunization with H.influenzae :Oral administration of formalin-killed nontypeable H.influenzae or outer membrane proteins with adjuvant increased murine IgA antibody activities in saliva, nasopharyngeal secretion and serum. ELISPOT assay showed that outer membrane-specific IgA secreting lymphocytes were significantly increased in nasopharyngeal mucosa as well as in intestinal lamina propria after oral immunization. Moreover, live H.influenzae inoculated into the nasopharynx were rapidly eliminated in mice having oral immunization. The results suggest that oral immunization enhanced mucosal immunity and the clearance of the nasopharynx, and that oral immunization with H.influenzae might be effective in preventing upper respiratory tract imfection such as otitis media and sinusitis. Less

1）PCR检测流感嗜血杆菌：应用PCR法检测有渗出性中耳炎患者的中耳积液中的流感嗜血杆菌，其P6-DNA的检出率明显高于常规培养法检测到的细菌。 DNA 也经常在腺样体中被发现。结果表明，中耳积液和鼻咽中流感嗜血杆菌的存在比以前更常见。 2)鼻咽部和腺样体黏膜免疫反应：采用ELISA法测定渗出性中耳炎患者鼻咽部分泌物中分泌型IgA抗体活性。流感嗜血杆菌抗体滴度与鼻咽部流感嗜血杆菌数量呈负相关。鼻咽部腺样淋巴细胞 ELISPOT 检测显示 IgA 和 IgG 抗体的存在。流感嗜血杆菌外膜蛋白特异性分泌细胞的数量与亚丁中流感嗜血杆菌的数量呈负相关。结果表明，流感嗜血杆菌在鼻咽部的定植可能受到分泌型 IgA 的抑制。鼻咽部，腺样体可能调节鼻咽部的粘膜免疫反应。3）影响口腔流感嗜血杆菌免疫：口服福尔马林灭活的不可分型流感嗜血杆菌或外膜蛋白佐剂可增加小鼠唾液、鼻咽分泌物和血清中IgA抗体的活性，ELISPOT检测显示小鼠外膜特异性IgA分泌淋巴细胞显着增加。口服免疫后鼻咽粘膜以及肠固有层中存在活流感嗜血杆菌。接种到鼻咽部的流感嗜血菌在口服免疫小鼠中被迅速消除。结果表明，口服免疫增强了粘膜免疫和鼻咽部的清除，口服流感嗜血杆菌可能有效预防中耳炎等上呼吸道感染。和鼻窦炎。