Prevalence and Significance of Anti-CD38 Autoantibody Detected in Patients with Type 2 Diabetes.

2 型糖尿病患者中检测到的抗 CD38 自身抗体的患病率和意义。

• 批准号: 11671121
• 负责人: TAMINATO Tomohiko
• 金额: $ 2.24万
• 依托单位: Kagawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671121/
• 关键词: CD38; AntiCD38 antibody; Diabetes mellitus; Insulin secretion; ADP-ribosyl cyclase; Cyclic ADP-ribose

The present study attempted to clarify the prevalence and significance of anti-CD38 antibody, which can be detected in sera from diabetic patients. Sera were collected from 184 type 2 diabetics. An ElA system was developed to detect anti-CD38 antibody using Keyhole Limpet Hemocyanin (KLH) -conjugated CD38 fragment peptides, 287-297 (N-CVKNPEDSSCT-C (Ag-1) and 241-255 (N-CSNNPVSVFWKTVSR-C (Ag-2), as antigens.Among 184 diabetics, anti-CD38 antibody was detected in 43 patients. Most of antibody positive sera reacted with Ag-1 and a part of those reacted with Ag-2. The clinical characteristics of antibody-positive patients were short-duration of diabetes, higher blood glucose level, less white blood cell counts, and receiving diet or oral hypoglycemic agents, rather than insulin. Antibody-positive patients were shown to have relatively higher level of serum and urinary C-peptide than type 1 diabetics, suggesting modest potential reservation of insulin secretion. Anti-CD38 antibody was not concordant with anti-GAD or anti-microsomal antibodies in those patients. Pretreatment of partially purified lgG from the antibody positive sera inhibited D-glucose-induced insulin release from isolated rat islets.These results suggest that anti-CD38 antibody has a significant diagnostic and prognostic values in the diagnosis of worsening of type 2 diabetes or progression to type 1 diabetes.

本研究试图阐明抗CD38抗体的患病率和意义，这可以在糖尿病患者的血清中检测到。从184种2型糖尿病患者中收集血清。开发了ELA系统，用于检测抗CD38抗体，使用钥匙孔Limpet Hemocyanin（KLH）偶联的CD38碎片肽，287-297（N-CVKNPEDSCTCCCT-C（AG-1）和241-255 -2），抗原。在184个糖尿病患者中，在43例患者中检测到抗CD38抗体。是糖尿病的短期，较高的血糖水平，较少的白细胞计数以及接受饮食或口服降血糖药，而不是胰岛素，而不是抗体阳性患者。 1糖尿病患者，表明胰岛素分泌的潜在保留性适度。这些结果表明，抗CD38抗体在诊断2型糖尿病的恶化或发展为1型糖尿病时具有显着的诊断和预后值。

项目成果

Taminato T, Ito T, Kato H.: "CD38 antigen (ADP-ribosyl cyclase) and Secretory mechanism of insulin secretion. (in Japanese)"Nippon Rinsho. 55 (suppl). 880-87 (1997)

Taminato T、Ito T、Kato H.：“CD38 抗原（ADP-核糖基环化酶）和胰岛素分泌的分泌机制。（日语）”Nippon Rinsho。

Ikegaya N, Yonemura K, Suzuki T, Kato-Ohishi H, Taminato T, Hishida A.: "Impairment of ventilatory response to metabolic acidosis in insulin-dependent diabetic patients with advanced nephropathy."Ren Fail.. 21 (5). 495-8 (1999)

Ikegaya N、Yonemura K、Suzuki T、Kato-Ohishi H、Taminato T、Hishida A.：“胰岛素依赖型糖尿病晚期肾病患者对代谢性酸中毒的通气反应受损。”Ren Fail.. 21 (5)。

Ikegaya N,Yonemura K,Suzuki T,Kato-Ohishi H,Taminato T,Hishida A.: "Impairment of ventilatory response to metabolic acidosis in insulin-dependent diabetic patients with advanced nephropathy."Ren Fail.. 21(5). 495-498 (1999)

Ikegaya N，Yonemura K，Suzuki T，Kato-Ohishi H，Taminato T，Hishida A.：“胰岛素依赖型糖尿病晚期肾病患者对代谢性酸中毒的通气反应受损。”Ren Fail.. 21(5)。

T.Taminato, H.Mizawa, T.Ito, H.Kato-Ohishi: "Prevalence and Significance of Anti-CD38 Autoantibody Detected in Patients with Type 2 Diabetes."(submitted).

T.Taminato、H.Mizawa、T.Ito、H.Kato-Ohishi：“2 型糖尿病患者中检测到的抗 CD38 自身抗体的患病率和意义”（已提交）。

Rydzewski A, Urano T, Nagai N, Takada Y, Kato-Ohishi H, Taminato T, Yoshimi T, Takada A.: "Diurnal variation in serum remnant-like lipoproteins, platelet aggregation and fibrinolysis in healthy volunteers"Haemostasis. 27. 305-14 (1997)

Rydzewski A、Urano T、Nagai N、Takada Y、Kato-Ohishi H、Taminato T、Yoshimi T、Takada A.：“健康志愿者血清残留样脂蛋白、血小板聚集和纤维蛋白溶解的昼夜变化”止血。