Relationship between molecular pathology of tumors and radiobiological parameters

肿瘤分子病理学与放射生物学参数的关系

• 批准号: 11670921
• 负责人: NISHIMURA Yasumasa
• 金额: $ 2.3万
• 依托单位: Kinki Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670921/
• 关键词: quiescent cells; radioresistance; cell cycle docetaxel; recruitment; erythropoietin; VEGF; angiogenesis; 放射線生物学; Ki-67標識率; PCNA標織率; G0細胞分画; 細胞周期; Docetaxel; Mild hyperthermia

The effect of docetaxel, which stops the cycling cells at G2/M boundary, on recruitment from G0 phase to cycling phase was investigated in vivo using SCCVII tumor of C3H/He mice. G0 fraction at 24, 48, 72, 120, h after administration of docetaxel (90 mg/kg) was measured by flow cytometry and anti-Ki-67 labeling methods. Decrease of G1 fraction and increase of G2/M fraction were evident 24 h and 48 h after treatment. On the other hand, G0 fraction did not change until 120 h. The present study indicates that docetaxel inhibits recruitment from G0 to cycling cells in a solid tumor.The role of erythropoietin (EPO) in angiogenesis of rat liver tumors was investigated using immunohistochemical methods. EPO-receptor was detected in liver tumors, although it was not detected in normal and cirrhotic hepatic cells. In addition, significant correlation was noted between intratumor EPO concentration and microvascular density of the tumor. The present study suggests that EPO is related to the angiogenesis of malignant hepatic tumors.

使用C3H/HE小鼠的SCCVII肿瘤研究了Docetaxel在G2/M边界处停止循环细胞对从G0相到循环阶段募集的影响。通过流式细胞仪和抗KI-67标记方法测量多西他赛（90 mg/kg）后24、48、72、120的G0级分。治疗后24小时和48小时，G1分数的降低和G2/M分数的增加是明显的。另一方面，G0分数直到120 h才改变。本研究表明，多西他赛在实体瘤中抑制从G0到循环细胞的募集。红细胞生成素（EPO）在使用免疫组织化学方法研究中研究了大鼠肝肿瘤的血管生成。在肝肿瘤中检测到EPO受体，尽管在正常和肝硬化肝细胞中未检测到它。另外，肿瘤内EPO浓度与肿瘤的微血管密度之间存在显着相关性。本研究表明，EPO与恶性肝肿瘤的血管生成有关。

项目成果

Nishimura Y., et al.: "Long-term results of fractionated Strontium-90 radiation therapy for pterygia."Int.J.Radiat.Oncol.Biol.Phys.. 46. 137-141 (2000)

Nishimura Y. 等人：“分次锶 90 放射治疗翼状胬肉的长期结果。”Int.J.Radiat.Oncol.Biol.Phys.. 46. 137-141 (2000)

Kokubo M,Nishimura Y, 他: "Analysis of the clinical benefit of intraoperative radiotherapy in patients undergoing macroscopically curative resection for pancreatic cancer."Int.J.Radiat.Oncol.Biol.Phys.. 48. 1081-1087 (2000)

Kokubo M、Nishimura Y 等人：“接受宏观治愈性胰腺癌切除术的患者术中放疗的临床益处分析。Int.J.Radiat.Oncol.Biol.Phys.. 48. 1081-1087 (2000) ）

Nemoto K,Nishimura Y, 他: "Treatment of superficial esophageal cancer by external radiation therapy alone : results of a multi-institutional experience."Int.J.Radiat.Oncol.Biol.Phys.. 46. 921-925 (2000)

Nemoto K、Nishimura Y 等人：“仅通过外部放射治疗治疗浅表性食管癌：多机构经验的结果。”Int.J.Radiat.Oncol.Biol.Phys.. 46. 921-925（ 2000)

Okuno Y.,Nishimura Y.,他: "Prognostic values of proliferating cell nuclear antigen(PCNA)and Ki-67 for definitive radiotherapy of esophageal squamous cell carcinomas"Br.J.Cancer. 80. 387-395 (1999)

Okuno Y.、Nishimura Y. 等人：“增殖细胞核抗原 (PCNA) 和 Ki-67 对食管鳞状细胞癌根治性放疗的预后价值”Br.J.Cancer. 80. 387-395 ( 1999）

Horii N, Nishimura Y, et al.: "Impact of neoadjuvant chemotherapy on Ki-67 and PCNA labeling indices for esophageal squamous cell carcinomas."Int.J.Radiat.Oncol.Biol.Phys.. (in press).

Horii N、Nishimura Y 等人：“新辅助化疗对食管鳞状细胞癌 Ki-67 和 PCNA 标记指数的影响”。Int.J.Radiat.Oncol.Biol.Phys.（出版中）。