Study on mechanism of reproductive toxicity and neurotoxicity of 1-bromopropane, a new alternative to chlorofluorocarbons

氯氟烃新替代品1-溴丙烷生殖毒性和神经毒性机制研究

基本信息

批准号：
11670367
负责人：
ICHIHARA Gaku
金额：
$ 2.5万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

(1) STUDY ON TOXIC MECHANISM OF 1-BROMOPROPANE Forty-four Wistar female rats were divided into four groups of elevens and exposed to 1-bromopropane at 200 ppm, 400 ppm and 800 ppm, for eight hours per day for twelve weeks. The control was given only fresh air. Results were as follows : 1) weakened muscle strength of limbs, 2) elongation of distal latency of rat tail, 3) decrease in motor nerve conduction velocity, 4) degeneration of myelin of peripheral nerve, 5) swelling of preterminal axons in gracile nucleus in medulla oblongata, 6) irregular banding of muscle fibers, 7) decreased epididymal sperm, 8) decreased motility of sperm, 9) increase in sperm with immature head, and 10) increased step 19 spermatid at post spermiation stages. The study shows that 1-bromopropane has severe dose-dependent neurotoxicity and also impairs spermiation.(2) BIOCHEMICAL STUDY ON BRAIN OF RATS EXPOSED TO 1-BROMOPROPANE Thirty-two Wistar male rats were divided into four groups and exposed to 1-bromopropane at 200, 400 and 800 ppm, eight hours per day for seven days. Total creatine kinase activity decreased in cerebrum, cerebellum, brainstem and spinal cord. Non protein free SH base decreased and total glutathione significantly decreased at cerebrum and cerebellum.(3) STUDY ON BIOMARKER OF 1-BROMOPROPANE EXPOSURE (a cooperative study with Dr.Valentine, Vanderbilt University, USA) Urine was collected from 29 workers exposed to 1-bromopropane. The urine sample was acidified and extracted with ethyl acetate. N-acetyl S-propylcystein was found as a major metabolite of 1-bromopropane. This might be a good indicator of 1-bromopropane exposure.

（1）对1-溴丙烷的毒性机制进行研究，将四十四个Wistar雌性大鼠分为四组高甲，并在200 ppm，400 ppm和800 ppm的情况下暴露于1-溴丙烷，每天八个小时，持续十二周。控制只给出新鲜空气。结果如下：1）肢体的肌肉强度弱，2）大鼠尾远端潜伏期的伸长，3）运动神经传导速度的降低，4）外周神经髓磷脂的变性，5）肉芽核核中比美质轴突肿胀的肿胀在髓质长肌中，6）肌肉纤维的不规则带，7）降低附子精子，8）精子的运动性降低，9）精子的增加，未成熟的头部和10）在精子后阶段增加了第19步的精子。研究表明，1-溴丙烷具有严重的剂量依赖性神经毒性，还会损害细胞。（2）将暴露于1-溴丙烷的大鼠大脑的生化研究将暴露于1-溴丙烷的三十二个雄性大鼠分为四组，并暴露于1-溴丙烷上。 200、400和800 ppm，每天八个小时，持续7天。大脑，小脑，脑干和脊髓的总肌酸激酶活性降低。非蛋白质无sH碱降低，总谷胱甘肽在大脑和小脑处显着降低。（3）对1-溴丙烷暴露的生物标志物研究（与美国范德比尔特大学Valentine博士进行的合作研究（一项合作研究），从29名工人中收集了29名工人 - 溴丙烷。将尿液样品酸化并用乙酸乙酯提取。发现N-乙酰基丙基菌是1-溴丙烷的主要代谢产物。这可能是1-溴丙烷暴露的良好指标。