Biological characteristics of aortic smooth muscle cells involving in lipid metabolism : the distribution and synthesis of apolipoprotein J and cholesteryl ester transfer protein.

主动脉平滑肌细胞参与脂质代谢的生物学特性：载脂蛋白J和胆固醇酯转运蛋白的分布与合成。

• 批准号: 11670197
• 负责人: ISHIKAWA Yukio
• 金额: $ 1.86万
• 依托单位: School of Medicine, Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670197/
• 关键词: Apolipoprotein J; Atherosclerosis; CETP; Immunohistochemistry; Smooth muscle cells; In situ hybridization

Immunohistochemical distribution of apolipoproteins in the abdominal aortas of 142 men, 15-34 years of age, collected in USA, was examined in relationship to serum VLDL+LDL, HDL cholesterol levels. The pattern of apoJ distribution was similar to that of apoA-I and E.The extent of apolipoprotein distribution in the intima and media increased with age and the stage of atherosclerosis. The infiltration of lipoprotein particles into aortic wall seems to be strongly associated with the progression of intimal lesions. ApoJ is produced by smooth muscle cells in the intima and has a role of reverse cholesterol transport in young subjects.In addition, the distribution and synthesis of cholesteryl ester transfer protein (CETP) were examined by immunohistochemistry and in situ hybridization. CETP was constantly distributed in the media, and it was recognized in the intimal smooth muscle cells within atheroslerotic lesions. CETP mRNA was also detected in the aortic smooth muscle cells.From these results, arterial smooth muscle cells positively participate in the removal of excessive cholesterol from the arterial wall through apoJ and CETP production.

对美国收集的 142 名 15-34 岁男性腹主动脉中载脂蛋白的免疫组织化学分布进行了检查，并与血清 VLDL+LDL、HDL 胆固醇水平相关。 apoJ的分布模式与apoA-I和E相似。载脂蛋白在内膜和中膜中的分布程度随着年龄和动脉粥样硬化的阶段而增加。脂蛋白颗粒向主动脉壁的浸润似乎与内膜病变的进展密切相关。 ApoJ由内膜平滑肌细胞产生，在年轻受试者中具有反向胆固醇转运的作用。此外，通过免疫组化和原位杂交检测胆固醇酯转移蛋白（CETP）的分布和合成。 CETP持续分布在中膜中，并在动脉粥样硬化病变内的内膜平滑肌细胞中被识别。在主动脉平滑肌细胞中也检测到了CETP mRNA。从这些结果来看，动脉平滑肌细胞通过apoJ和CETP的产生积极参与从动脉壁去除过量的胆固醇。

项目成果

石川由起雄 他: "血行力学負荷時の脈管組織における膠原線維代謝及び形態の変貌"脈管学. 40. 15-24 (2000)

Y. Ishikawa 等人：“血流动力学负荷期间血管组织中胶原纤维代谢和形态的变化”Angiology 40. 15-24 (2000)。

石川由起雄 他: "螺旋型膠原線維とmatrix metalloproteinases(MMPs)"動脈硬化. 26. 179-183 (1998)

Y. Ishikawa 等人：“螺旋胶原纤维和基质金属蛋白酶 (MMP)” 动脉硬化。 26. 179-183 (1998)

Ishikawa Y,Ishii T,Akasaka Y, et al.: "Immunolocalization of apolipoproteins in aortic atherosclerosis in American youths and young adults."Atherosclerosis. (in press).

Ishikawa Y、Ishii T、Akasaka Y 等人：“美国青少年和年轻人主动脉粥样硬化中载脂蛋白的免疫定位。”动脉粥样硬化。

Ishikawa Y,et al: "Collagen alteration in the vascular remodeling by haemodynamic factors"Virchows Arch. (in press).

Ishikawa Y 等人：“血流动力学因素导致的血管重塑中的胶原蛋白改变”Virchows Arch。

Ishikawa Y,Akasaka Y, et al.: "Sequential changes in localization of repair-related protein in the different stages of myocardial infarction."Histopathology. 37. 546-554 (2000)

Ishikawa Y，Akasaka Y，等人：“心肌梗死不同阶段修复相关蛋白定位的连续变化。”组织病理学。