Molecular pathological research of the genesis and development of serrated adenoma of the colorectum

结直肠锯齿状腺瘤发生发展的分子病理学研究

• 批准号: 11670170
• 负责人: AJIOKA Yoichi
• 金额: $ 1.66万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670170/
• 关键词: colorectal adenoma; serrated adenoma; K-ras; APC; p53; 金居歯状腺腫

Genetica alterations were examined for 63 serrated adenomas(SA)and 10 carcinoma in serrated adenoma(CA/SA).1. K-ras codon 12 mutations were found only in 6.3%(4/63)of SA, and two of them were mixed polyp consisted of admixture of SA and ordinary tubular adenoma. No mutation was found in CA/SA.2. Alterations of APC gene were found in one SA polyp, alone.3. p53 protein overexpression was examined using anti-human p53 protein monoclonal antibody(PAb1801). Overexpression was noticed in 3.2%(2/63)of SA and 80%(8/10)of carcinomatous component of CA/SA.4. Alteration of APC and K-ras genes may not play a role in genesis and development of SA.however, p53 alteration would be related in carcinogenesis of SA.

对63例锯齿状腺瘤(SA)和10例锯齿状腺瘤癌(CA/SA)进行了遗传学检查。 1． K-ras密码子12突变仅在6.3%(4/63)的SA中发现，其中2例为SA与普通管状腺瘤混合而成的混合息肉。 CA/SA.2未发现突变。 1例SA息肉中仅发现APC基因变异。 3．使用抗人p53蛋白单克隆抗体(PAb1801)检查p53蛋白过度表达。 3.2％(2/63)的SA和80％(8/10)的CA/SA癌变成分中观察到过表达。4。 APC和K-ras基因的改变可能与SA的发生、发展无关，而p53的改变可能与SA的癌变有关。

项目成果

Biemer-Huttmann AE.: "Immunohistochemical staining patterns of MUC1, MUC2, MUC4 and MUC5AC mucins in hyperplastic polyps, serrated adenomas, and traditional adenomas of the colorectum"Journal of Histochemistry and Cytochemistry. 47. 1039-1048 (1999)

Biemer-Huttmann AE.：“结直肠增生性息肉、锯齿状腺瘤和传统腺瘤中 MUC1、MUC2、MUC4 和 MUC5AC 粘蛋白的免疫组织化学染色模式”组织化学和细胞化学杂志。

Ajioka Y.: "Surgery of the colorectum and anus"Asakura-Shoten. 500 (1999)

Ajioka Y.：“结直肠和肛门手术”朝仓书店。

Biemer-Huttmann AE.: "Immunohistochemical staining patterns of MUC1, MUC2, MUC4 and MUC5AC mucins in hyperplastic polyps, serrated adenomas, and traditional adenomas of the colorectum."J.Histochem Cytochem. 47. 1039-1048 (1999)

Biemer-Huttmann AE.：“结直肠增生性息肉、锯齿状腺瘤和传统腺瘤中 MUC1、MUC2、MUC4 和 MUC5AC 粘蛋白的免疫组织化学染色模式。”J.Histochem Cytochem。

Taguchi Y.: "Clinicopathological study on serrated adenomas of the colorectum. (in Japanese)"Jpn.J.Gastroeetrology. 42. 247-257 (2000)

田口 Y.：“结直肠锯齿状腺瘤的临床病理学研究。（日语）”Jpn.J.Gastroeetrology。

Bimer-Hattmann AE: "Immunehistochemical staining patterns of MUC1, MUC2, MUC4 and MUCSAC mucins in hyperplastic polyps, serrated a denonas, and traditional adenomas of the colorectum"J.Histochem Cijtochem. 47. 1039-1048 (1999)

Bimer-Hattmann AE：“增生性息肉、锯齿状腺瘤和结直肠传统腺瘤中 MUC1、MUC2、MUC4 和 MUCSAC 粘蛋白的免疫组织化学染色模式”J.Histochem Cijtochem。