Antioxidant activities of natural 9-cis β-carotene of Donliella bardawil on human neutrophil ROS production

Donliella bardawil 天然 9-cis β-胡萝卜素对人中性粒细胞 ROS 产生的抗氧化活性

• 批准号: 11357004
• 负责人: SUGAWARA Kazuo
• 金额: $ 9.91万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11357004/
• 关键词: β -caroten; Donaliella bardawil; neutrophil; reactive oxygen species; chemiluminescence; antioxidant; 窒素酸化物

Much attention has been focused on the biological activity of geometrical isomers of β -carotene. In the present study, the in vitro antioxidant activities of a natural 9-cisβ-carotene purified from Donaliella bardawil and synthetic all-transβ -carotene were studied using enhanced human neutrophil chemiluminescence upon stimulation with opsonized zymosan. Lucigenin-dependent chemiluminescence was used for largely measuring superoxide anion (O_2) production, luminal-dependent chemiluminescence with sodium azide (NaN_3) and horseradish peroxidase (HRP) was used for measuring hydrogen peroxide (H_2O_2) production, and luminal-dependent chemiluminescence without oxidant metabolism modulator was used for determination of myeloperoxydase (MPO)-mediated production. With β -carotene concentration of 1.4 μM, neutrophils showed a decrease in lucigenin-dependent chemiluminescence to 75 % and 78 % of controls (P<0.05) by natural 9-cis β -carotene and synthetic all-trans β-carotene, respectively. With β-carotene concentration of 1.86 μM, neutrophils also showed a decrease in luminol-dependent chemiluminescence in the presence of NaN_3 and HRP to 86 % and 81 % of controls (P< 0.05) by natural 9-cis β -carotene and synthetic all-trans β-carotene, respectively. On the other hand, neutrophils did not show a statistically significant difference in any chemiluminescence assay by the same concentration of natural 9-cis β -carotene and synthetic a all-trans B -carotene. These results suggest that, up to the concentration of 1.86μM, natural 9-cis B -carotene and synthetic all-trans β -carotene exert equal antioxidant activities assessed by enhanced human neutrophil chemiluminescence.

非常关注β-胡萝卜素的几何异构体的生物识别活性。在本研究中，使用增强的人类嗜中性粒细胞化学发光，在用zymassan刺激后，使用增强的人类嗜中性粒细胞化学发光研究了从Donaliella bardawil纯化的天然9-CISβ-胡萝卜素的体外抗氧化活性。使用lucigenin依赖性化学发光来主要测量超氧化物阴离子（O_2）的产生，用叠氮化钠（NAN_3）和辣根过氧化物酶（HRP）用于测量过氧化物（H_2O_2）生产，以及模型依赖性化学剂的氧化氢（NAN_3）和辣根过氧化物酶（HRP）的腔内依赖性化学发光和辣根过氧化物酶（HRP）。骨髓过氧化物酶（MPO）介导的产生。随着天然9-CISβ-胡萝卜素和合成的All-transβ-胡萝卜素，中性粒细胞的β-胡萝卜素浓度为1.4μM，嗜中性粒细胞表现出lucigenin依赖性化学发光的降低至75％和78％的对照（P <0.05）。在β-胡萝卜素浓度为1.86μM的情况下，中性粒细胞在NAN_3和HRP存在下也显示出依赖性的化学发光，分别由天然9-cisβ-可胡萝卜素和合成的全抗All-Transβ-碳的对照组分别为86％和81％（P <0.05）。另一方面，中性粒细胞在任何化学发光评估中没有通过相同浓度的天然9 -CISβ-胡萝卜素和合成A全频率B-胡萝卜素显示出统计学上的显着差异。这些结果表明，通过增强的人类嗜中性粒细胞发光评估的浓度达到1.86μm，天然9 -CIS B-碳酸盐和合成的全反型β-胡萝卜素均具有相等的抗氧化活性。

项目成果

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