MECHANISMS APPROACH TO GaAlAs DIODE LASER EFFECTS ON PRODUCTION OF REACTIVE OXYGEN SPECIES FROM HUMAN NEUTROPHILS AS A MODEL FOR THERAPEUTIC MODALITY AT CELLULAR LEVEL

GaAlAs 二极管激光对人类中性粒细胞活性氧产生影响的机制方法作为细胞水平治疗方式的模型

基本信息

批准号：
03454200
负责人：
SUGAWARA Kazuo
金额：
$ 2.43万
依托单位：
HIROSAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

There have been many reports on the applications of low reactive level laser(LLL) therapy for pain attenuation of pain removal. Our group has reported previously on the effects of in vitro irradiation of LLL particularly on the phagocytic activity of human neutrophils, using luminol-dependent chemiluminescence(LmCL) for measurement of reactive oxygen species(ROS) production from human neutrophils. But the mechanisms of the attenuation of phagocytic activity of neutrophils by LLL irradiation is not yet full understood.In this study, we used luminol-dependent and lucigenin-dependent chemiluminescence(LgCL) for detection of affected ROS producing process of human neutrophils by LLL irradiation. Two soluble action stimuli, N-formyl-Met-Leu-Phe(fMLP) and phorbol myristate acetate(PMA) were used to avoid the possible influence of lag-time from recognition to uptake of particles at the ROS production.In case of using fMLP as a stimulus, the maximum luminescence intensity of LmCL was increased but LgCL luminescence was decreased by LLL irradiation. When PMA was used as a stimulus, the times to reach the maximum luminescence intensity of LmCL and LgCL were shortend by LLL irradiation but there was no effect on the maximum luminescence intensity of both.These results suggest that LLL irradiation enhances the ROS production activity of human neutrophils by the activation of the superoxide converting system, the active element in which is mainly myeloperoxidase. LLL irradiation enable a more rapid activation of the superoxide production system, NADPH-oxidase.

关于低反应激光（LLL）疗法在减轻疼痛或消除疼痛方面的应用已有许多报道。我们课题组之前曾报道过 LLL 体外照射对人中性粒细胞吞噬活性的影响，特别是对人中性粒细胞吞噬活性的影响，利用鲁米诺依赖性化学发光（LmCL）测量人中性粒细胞产生的活性氧（ROS）。但LLL照射减弱中性粒细胞吞噬活性的机制尚不完全清楚。本研究采用鲁米诺依赖性和光泽精依赖性化学发光(LgCL)技术检测LLL照射对人中性粒细胞ROS产生过程的影响。。使用两种可溶性作用刺激，N-甲酰基-Met-Leu-Phe（fMLP）和佛波醇肉豆蔻酸酯（PMA），以避免ROS产生时从识别到摄取颗粒的滞后时间可能产生的影响。 fMLP作为刺激物，LmCL的最大发光强度增加，但LgCL的发光强度因LLL照射而减少。当使用PMA作为刺激物时，LLL照射缩短了LmCL和LgCL达到最大发光强度的时间，但对两者的最大发光强度没有影响。这些结果表明，LLL照射增强了LmCL和LgCL的ROS产生活性。人体中性粒细胞通过超氧化物转换系统的激活，其中的活性成分主要是髓过氧化物酶。 LLL 照射能够更快速地激活超氧化物生产系统 NADPH 氧化酶。