Biochemical studies on the quantum mechanical successive reaction system in organized molecular assemblies in biological materials.

生物材料中有序分子组装体中量子力学连续反应系统的生化研究。

• 批准号: 11223206
• 负责人: MIMURO Mamoru
• 金额: $ 26.94万
• 依托单位: YAMAGUCHI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11223206/
• 关键词: Photosynthesis; Energy transfer; successive reaction system; Reaction center; novel pigments; 量子化反応; 超高速分光法; カロテノイド; 緩和過程; 連鎖反応; 量子論; アンテナ; 緑色光合成細菌; クロロゾーム; フェムト秒分光

The successive reaction system in the primary processes of photosynthesis was analyzed. We used biological materials, and did not construct the artificial system. We obtained the following four major results.(1) We analyzed the energy transfer process in a newly found cyanobacterium Acaryochloris marina that is known to contain Chl d. The energy level of antenna Chl a is lower than the energy trap, however the up-hill energy transfer process was proved to function in this organism, indicating a new concept in the antenna system. We also found a new species that contains Chl d, and this leads to the final conclusion on the presence of Chl d in red algae.(2) We proved that the spatial orientation of the special pair in the newly found Zn-BChl a containing photosynthetic bacterium Acidiphilium rubrum was different from that in the purple bacteria. A weak interaction between Zn-BChl a's suggested the modification in the reaction kinetics.(3) we applied the normal mode analysis to the antenna protein of cyanobacteria, that is, phycobiliproteins, and concluded that the protein structure is essential for stabilizing the electronic state of chromophores that engages in the successive reaction system.(4) We analyzed the excitation relaxation processes of carotenoids that function as an efficient antenna in algae, and found that very fast vibrational relaxationin the S2 state is essential for the antenna function.

分析了光合作用的主要过程中的连续反应系统。我们使用了生物材料，并且没有构建人工系统。我们获得了以下四个主要结果。（1）我们分析了新发现的氰基氯氯氯便氯乙烷码头中的能量转移过程，该杂物含有CHL d。天线CHL A的能级低于能量陷阱，但是，向上沿沿线的能量转移过程被证明在该生物体中起作用，表明天线系统中的新概念。我们还发现了一个包含CHL D的新物种，这导致了关于红藻中CHL D存在的最终结论。（2）我们证明，在新发现的Zn-BCHL A中，特殊对的空间取向含有光合细菌酸性细菌rubrum与紫色细菌不同。 Zn-BCHL A之间的相互作用较弱提示反应动力学中的修饰。（3）我们将正常模式分析应用于蓝细菌的天线蛋白，即植物蛋白质，并得出结论，并得出结论，蛋白质结构对于稳定了连续反作机的刺激性反作机的电子状态至关重要。在藻类中充当有效的天线，发现S2态在天线功能中至关重要。

项目成果

M. Mimuro, T. Ookubo, D. Takahashi, T. Sakawa, S. Akimoto, I. Yamazaki, and H. Miyashita: "Unique fluorescence properties of a cyanobacterium Gloeobacter violaceus PCC 7421: Reasons for absence of the long-wavelength PS I Chl a fluorescence at -196℃."Plan

M. Mimuro、T. Ookubo、D. Takahashi、T. Sakawa、S. Akimoto、I. Yamazaki 和 H. Miyashita：“蓝细菌 Gloeobacter violaceus PCC 7421 的独特荧光特性：缺乏长波长 PS 的原因I Chl a 在-196℃发出荧光。"计划

S. Akimoto, I. Yamazaki, S. Takaichi, and M. Mimuro: "Excitation relaxation dynamics of linear carotenoids"J. Luminescence. 87-89. 797-799 (2000)

S. Akimoto、I. Yamazaki、S. Takaichi 和 M. Mimuro：“线性类胡萝卜素的激发弛豫动力学”J。

Mimuro, M., H.Kikuchi: "Antenna Systems and Energy Transfer in Cyanophyta and Rhodophyta"Antenna book(B.R.Green,and W.W.Parson eds.). (in press). (2002)

Mimuro, M., H.Kikuchi：“蓝藻和红藻中的天线系统和能量转移”天线书（B.R.Green 和 W.W.Parson 编辑）。

T. Ishii, K. Uehara, Y. Ozaki, and M. Mimuro: "The effects of pH and ionic strength on the aggregation of bacteriochlorophyll c in aqueous organic media: possibility of two kinds of aggregation forms."Photochem. Photobiol.. 70. 760-765 (1999)

T. Ishii、K. Uehara、Y. Ozaki 和 M. Mimuro：“pH 和离子强度对水性有机介质中细菌叶绿素 c 聚集的影响：两种聚集形式的可能性。”Photochem。

S. Akimoto, I. Yamazaki, T. Sakawa, and M. Mimuro: "Temperature effects on excitation relaxation dynamics of the carotenoid, B-carotene and its analogue, β-apo-8'-carotenal, probed by the femtosecond fluorescence spectroscopy"J. Phys. Chem.. (in press). (

S. Akimoto、I. Yamazaki、T. Sakawa 和 M. Mimuro：“通过飞秒荧光光谱探测温度对类胡萝卜素、B-胡萝卜素及其类似物 β-apo-8-胡萝卜醛的激发弛豫动力学的影响“J. Phys. Chem..（正在出版）。