Origin and phylogeny of symmetry structure of protein assemblies-requirement from function or phyloggenic consequence?

蛋白质组装体对称结构的起源和系统发育——功能或系统发生结果的要求？

基本信息

批准号：
08309010
负责人：
MIMURO Mamoru
金额：
$ 7.17万
依托单位：
YAMAGUCHI UNIVERSITY (1997)Okazaki National Research Institutes (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

A symmetry structure of protein assemblies is sometimes found in the systems of photosynthesis, an energy conversion system and vision, a signal transduction system. Significance of such symmetry structure was investigated from the following two points ; (1) necessity for function and (phylogeny of proteins having different origins. The major points for analyzes were (1) interaction between prosthetic groups and (2) domain structure and/or module structure of proteins. Analyzes were carried out on photosynthetic reaction center (C_2 symmetry), antenna system (maimly C_3 symmetry), rhodopsin family (C_3 symmetry), and also pseudo C_3 symmetry in ATPase, the last of which might have a significance as well as the symmetry structure.In the case of photosynthetic antenna system, the symmetry structure is an easy way to increase a number of pigments for light energy collection (or absorption). However, it is very rare that this kind of structure can be assigned to a consequence for the expression of function. For example, phycobiliproteins in cyanobacteria and red algae contain two additional helices at the N terminus of a putative ancestral protein that is assigned to the globin family. Owing to these two helices, phycobiliproteins acquired the symmetry structure different from that of globin families and at the same time, an interaction between chromophores was realized, which was necessity for antennafunction.In the case of ATPase, breakdown of symmetry structure is shown to be necessity for the expression of function, which was proved by the recent analysis of the three-dimensional structure.In conclusion, the current subject is concerned with the structure-function relationship of the protein assemblies whose structural data are accumulating very rapidly, and thus it should be continued for further analyzes.

有时在光合作用系统，能量转换系统和视觉，信号转导系统的系统中发现蛋白质组件的对称结构。从以下两个点研究了这种对称结构的重要性。 (1) necessity for function and (phylogeny of proteins having different origins. The major points for analyzes were (1) interaction between prosthetic groups and (2) domain structure and/or module structure of proteins. Analyzes were carried out on photosynthetic reaction center (C_2 symmetry), antenna system (maimly C_3 symmetry), rhodopsin family (C_3 symmetry), and also pseudo C_3 ATPase中的对称性可能具有显着性和对称结构。在光合作用天线系统的情况下，对称结构是增加许多颜料以收集光能（或吸收）的简便方法。藻类在指定的祖先蛋白的N末端中包含两个螺旋，由于这两个螺旋而分配给球蛋白家族，phycobiliprotein获得了与球蛋白家族的对称结构，并在同一时间的相互作用中呈现了伪团的相互作用，这是必需的。功能，这是通过对三维结构的最新分析证明的。总之，当前的受试者与蛋白质组件的结构 - 功能关系有关，蛋白质组件的结构数据非常迅速地积累，因此应继续进行进一步的分析。