Highly Sensitive Analytical Studies for Biological Compounds Based on Chemiluminescence Reaction

基于化学发光反应的生物化合物高灵敏度分析研究

• 批准号: 60571027
• 负责人: MAEDA Masako
• 金额: $ 1.09万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60571027/
• 关键词: Lucigenin; NAD(P)H; Biological Compounds; Chemiluminescence Reaction; Chemiluminescence; Chemiluminescence HPLC; デヒドロゲナーゼ; 酵素免疫測定法; グルコース; イソルミノール; m-POD; 1-MPMS; ATP

Chemiluminescence assay is attractive analytical method in the field of clinical chemistry, because of their sensitivity, rapidity, and wide dynamic range. The chemiluminescent agents most commonly used for analytical purposes are luminol and its derivatives and lucigenin. Peroxyoxalate-sensitized chemiluminescence is also applied to determination of <H_2> <O_2> and fluorescent compounds. We also developed new chemiluminescent reaction for NAD(P)H by using l-methoxyphenazinium methosulfate-isoluminol-m-POD.These chemiluminescent reaction were applied to determination of biological substances such as steroids, bile acids, thyroxine, glucose and various enzyme activity in biological fluids by using high performance liquid chromatographic, flow injection analysis or enzyme immunoassay techniques.

化学发光分析因其灵敏度、快速性和宽动态范围而成为临床化学领域颇具吸引力的分析方法。最常用于分析目的的化学发光剂是鲁米诺及其衍生物和光泽精。过草酸敏化化学发光还应用于<H_2>、<O_2>和荧光化合物的测定。我们还利用l-甲氧基吩嗪鎓甲基硫酸盐-异鲁米诺-m-POD开发了新的NAD(P)H化学发光反应。这些化学发光反应应用于测定类固醇、胆汁酸、甲状腺素、葡萄糖和各种酶活性等生物物质。使用高效液相色谱、流动注射分析或酶免疫分析技术对生物液体进行分析。

项目成果

Akio Tsuji: J.Bioluminescence and Chemiluminescence. 3. (1987)

Akio Tsuji：J.生物发光和化学发光。

Masako Maeda and Akio Tsuji: "Chemiluminescence with Lucigenin as Pre-column Reagent in HPLC of Corticosteroids and p-Nitrophenacyl Esters" J. Chromatography. 352. 213-220 (1986)

Masako Maeda 和 Akio Tsuji：“在皮质类固醇和对硝基芬酯的 HPLC 中使用光泽精作为柱前试剂的化学发光”J. 色谱法。

Akio Tsuji, Masako Maeda and Hidetoshi Arakawa: CRS Press (USA). Bioluminescence and Chemiluminescence ; Instruments and Applications, 240 (1985)

Akio Tsuji、Masako Maeda 和 Hidetoshi Arakawa：CRS Press（美国）。

Hidetoshi Arakawa: Clinical Chemistry. 31. 430-434 (1985)

荒川英俊：临床化学。

Masako Maeda and Akio Tsuji: "Fluorescence and Chemiluminescence Determination of Steroid and Bile Acid Sulfates with Lucigenin by Flow-Injection Analysis Based on Ion-Pair Solvent Extraction" Analyst. 110. 665-668 (1985)

Masako Maeda 和 Akio Tsuji：“基于离子对溶剂萃取的流动注射分析用光泽精对类固醇和胆汁酸硫酸盐进行荧光和化学发光测定”分析师。