Effects of ELF magnetic field and induced currents on cell growth or differentiation of cultured osteoblasts.

ELF磁场和感应电流对培养成骨细胞的细胞生长或分化的影响。

基本信息

  • 批准号:
    10837010
  • 负责人:
  • 金额:
    $ 2.05万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

We have obtained some research results from the experiments for the effects of exposure to ELF magnetic fields(3mT) as following.(1) Significant differencies existed between the exposure and control groups of cultured osteoblasts(MC3T3 E-1) as to cellular ALP activities and protein contents at 30 and 60 Hz during the application of magnet for 25 days, respectively. The stimulatory effects of 60 Hz magnetic fields for protein synthesis of osteoblasts were observed concerning the accumulation of collagen and non-collagen protein in the cultures for 14 days in particularly.(2) No significant differences were observed between the exposure and control groups on the accumulation of collagen and non-collagen protein due to the electric currents at 60 Hz during 25 days.(3) The effects of exposure to ELF magnetic field for short time on growing or differentiated osteoblasts.1) The intracellular Ca2+ concentration ([Ca2+]i) of growing osteoblasts after addition of bradyknin(BK) in the culture media with or without of Ca2+ were examined. The [Ca2+]i increased instantly from the resting level on addition of BK, reaching a maxmal value in normal medium with Ca2+. When the free Ca2+ was chelated in the culture medium by addition of EGTA, the peak value was only half of that in normal medium after the stimulation of BK. Exposure to magnetic field for 24 hours 4t the absence of Ca2+ strongly inhibited the increase in [Ca2+]i.2) The intracellular Ca2+ concentration [Ca2+]i of growing osteoblasts after addition of endothelin-1(ET) in the culture media with or without of Ca2+ were examined. [Ca2+]i of unexposed cells increased instantly by addition of ET, but the increasing of [Ca2+]i in exposed cells by reagent was inhibited significantly as BK treatment. When the differetiated cells were exposed to magnetic field, the increasing of [Ca2+]i by ET stimulation was accelerated significantly in contrast to the growing cells.
我们通过实验得到了以下一些关于极低频磁场(3mT)暴露影响的研究结果:(1)暴露组和对照组培养的成骨细胞(MC3T3 E-1)细胞ALP活性存在显着差异。分别在 30 赫兹和 60 赫兹施加磁铁 25 天期间的蛋白质含量。观察到60 Hz磁场对成骨细胞蛋白质合成的刺激作用,特别是在14 d的培养物中胶原蛋白和非胶原蛋白的积累方面。(2)暴露组和对照组之间没有观察到显着差异。 25天内60 Hz电流导致胶原蛋白和非胶原蛋白的积累。(3)短时间暴露于ELF磁场对成骨细胞生长或分化的影响。1)检测在含有或不含有Ca2+的培养基中添加缓激肽(BK)后生长的成骨细胞的细胞内Ca2+浓度([Ca2+]i)。添加 BK 后,[Ca2+]i 从静止水平立即增加,在含 Ca2+ 的正常培养基中达到最大值。当添加EGTA螯合培养基中的游离Ca2+时,BK刺激后峰值仅为正常培养基中的一半。暴露于磁场24小时4t,没有Ca2+强烈抑制[Ca2+]i的增加。2)在培养基中添加内皮素-1(ET)后生长的成骨细胞的细胞内Ca2+浓度[Ca2+]i没有检查Ca2+。添加ET后,未暴露细胞的[Ca2+]i立即增加,但BK处理显着抑制了试剂暴露细胞中[Ca2+]i的增加。当分化的细胞暴露于磁场时,与生长中的细胞相比,ET刺激引起的[Ca2+]i的增加显着加速。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Park,Ki-Ho: "Effects ofELF magnetic fields on collegen synthesis in osteobiasts." Electricty magnetism in Biology and Medicine,F.Bersani,ed.457-460 (1999)
Park, Ki-Ho:“ELF 磁场对成骨细胞中大学素合成的影响。”
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    0
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Park,K.H.,Yamaguchi,H.et al.: "Effects of ELF magnetic fields on collagen synthesis in osteoblasts"In:Electricity and Magnetism in Biology and Medicine. 457-460 (1999)
Park, K.H., Yamaguchi, H.等人:“ELF 磁场对成骨细胞胶原蛋白合成的影响”,见:生物学和医学中的电和磁。
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    0
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Ikehara.T.,Yamaguchi,H.et al.: "Effects of electromagnetic fields on membrane ion transport of cultured cell"J.Med.Inv.. 45. 47-56 (1998)
Ikehara.T.,Yamaguchi,H.et al.:“电磁场对培养细胞膜离子传输的影响”J.Med.Inv.. 45. 47-56 (1998)
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Ikehara, T., Park, K.-H., Yamaguchi, H., Hosokawa, K., Yoshizaki, K., Miyamoto, H., Aizawa, K., Kinouchi, Y.: "Effects on RB+(K+) uptake of HeLa cells in a high K+ medium of exposure to a switched 1.7 Tesla magnetic field."Bioelectromagnetics. 21, (in pre
Ikehara, T.、Park, K.-H.、Yamaguchi, H.、Hosokawa, K.、Yoshizaki, K.、Miyamoto, H.、Aizawa, K.、Kinouchi, Y.:“对 RB (K ) 的影响
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Ikehara, T., Park, K.-H., Houchi, H., Yamaguchi, H., Hosokawa, K., Shono, M., Minakuchi, K., Tamaki, T., Kinouchi, Y., Yoshizaki, K., Miyamoto, H.: "Effects of a time-varying strong magnetic field on transient increase in cytosolic free Ca2+ induced by br
池原,T.,公园,K.-H.,侯内,H.,山口,H.,细川,K.,庄野,M.,水口,K.,玉木,T.,木之内,Y.,吉崎,
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YAMAGUCHI Hisao其他文献

YAMAGUCHI Hisao的其他文献

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{{ truncateString('YAMAGUCHI Hisao', 18)}}的其他基金

Development of appropriate prescription for health using the simultaneous telemetry method of cerebral blood flow velocity and electrocardiogram under physical exercise.
利用体育运动下脑血流速度和心电图同步遥测方法制定适当的健康处方。
  • 批准号:
    15500498
  • 财政年份:
    2003
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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相似海外基金

Occupational Exposure to ELF Electromagnetic Fields
职业暴露于极低频电磁场
  • 批准号:
    12450111
  • 财政年份:
    2000
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Effects of magnetic fields on many functions of human and other mammalian cells and proposal of guidelines and Standard for Human Health
磁场对人类和其他哺乳动物细胞许多功能的影响以及人类健康指南和标准的提议
  • 批准号:
    12558062
  • 财政年份:
    2000
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Assessment of Occupational Exposure to ELF Field and Its Interaction with Human Body
职业暴露于极低频场及其与人体相互作用的评估
  • 批准号:
    11834010
  • 财政年份:
    1999
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Quantification of ELF electric and magnetic fields in residential areas.
住宅区 ELF 电场和磁场的量化。
  • 批准号:
    10837012
  • 财政年份:
    1998
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study of improvement of electrical social environment on the electromagnetic field generated with high density electric energy transportation.
高密度电能输送产生的电磁场改善电力社会环境的研究
  • 批准号:
    10450108
  • 财政年份:
    1998
  • 资助金额:
    $ 2.05万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
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