Effects of ELF magnetic field and induced currents on cell growth or differentiation of cultured osteoblasts.

ELF磁场和感应电流对培养成骨细胞的细胞生长或分化的影响。

基本信息

批准号：
10837010
负责人：
YAMAGUCHI Hisao
金额：
$ 2.05万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We have obtained some research results from the experiments for the effects of exposure to ELF magnetic fields(3mT) as following.(1) Significant differencies existed between the exposure and control groups of cultured osteoblasts(MC3T3 E-1) as to cellular ALP activities and protein contents at 30 and 60 Hz during the application of magnet for 25 days, respectively. The stimulatory effects of 60 Hz magnetic fields for protein synthesis of osteoblasts were observed concerning the accumulation of collagen and non-collagen protein in the cultures for 14 days in particularly.(2) No significant differences were observed between the exposure and control groups on the accumulation of collagen and non-collagen protein due to the electric currents at 60 Hz during 25 days.(3) The effects of exposure to ELF magnetic field for short time on growing or differentiated osteoblasts.1) The intracellular Ca2+ concentration ([Ca2+]i) of growing osteoblasts after addition of bradyknin(BK) in the culture media with or without of Ca2+ were examined. The [Ca2+]i increased instantly from the resting level on addition of BK, reaching a maxmal value in normal medium with Ca2+. When the free Ca2+ was chelated in the culture medium by addition of EGTA, the peak value was only half of that in normal medium after the stimulation of BK. Exposure to magnetic field for 24 hours 4t the absence of Ca2+ strongly inhibited the increase in [Ca2+]i.2) The intracellular Ca2+ concentration [Ca2+]i of growing osteoblasts after addition of endothelin-1(ET) in the culture media with or without of Ca2+ were examined. [Ca2+]i of unexposed cells increased instantly by addition of ET, but the increasing of [Ca2+]i in exposed cells by reagent was inhibited significantly as BK treatment. When the differetiated cells were exposed to magnetic field, the increasing of [Ca2+]i by ET stimulation was accelerated significantly in contrast to the growing cells.

我们已经从实验中获得了一些研究结果，如以下是暴露于ELF磁场（3MT）的作用。（1）在磁铁施加25天的时间内，在30和60 Hz的情况下，培养成骨细胞（MC3T3 E-1）的暴露和对照组之间存在显着差异。 The stimulatory effects of 60 Hz magnetic fields for protein synthesis of osteoblasts were observed concerning the accumulation of collagen and non-collagen protein in the cultures for 14 days in particularly.(2) No significant differences were observed between the exposure and control groups on the accumulation of collagen and non-collagen protein due to the electric currents at 60 Hz during 25 days.(3) The effects of exposure to ELF magnetic field for 1）在培养或不含Ca2+的培养基中添加了Bradykhin（bk）后，在添加了bradykhin（bk）后，研究了成骨细胞的细胞内Ca2+浓度（[Ca2+] i）的短时间。 [Ca2+] i在添加BK时立即从静止水平增加，在具有Ca2+的正常培养基中达到最大值。当通过添加EGTA将游离Ca2+螯合在培养基中时，在BK刺激后，在正常培养基中的峰值仅是一半。暴露于磁场24小时4T不存在Ca2+的不存在会严重抑制[Ca2+] I.2）在添加或不使用Ca2+的培养基中添加内皮素-1（ET）后，在添加了内皮素-1（ET）后，细胞内Ca2+浓度[Ca2+] I [Ca2+] I的增加[Ca2+] I的增加。 [Ca2+]未暴露细胞的I立即通过添加ET立即增加，但是通过试剂在暴露细胞中的[Ca2+] I的增加被显着抑制为BK处理。当不同导致的细胞暴露于磁场时，与生长细胞相比，通过ET刺激增加了[Ca2+] I的增加。