Effects of magnetic fields on many functions of human and other mammalian cells and proposal of guidelines and Standard for Human Health

磁场对人类和其他哺乳动物细胞许多功能的影响以及人类健康指南和标准的提议

基本信息

批准号：
12558062
负责人：
IKEHARA Toshitaka
金额：
$ 8.64万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1. Exposure to the time-varying 1.5 T magnetic field did not affect Ca^<2+> influx across plasma membrane of bovine adrenal chromaffin cells, but strongly inhibited Ca^<2+> release from intracellular Ca^<2+> stores induced by addition of variety of neurotransmitters. Ca^<2+> release from Ca^<2+> stores via ryanodine receptor also suppressed by the exposure.2. Exposure to the strong magnetic field strongly suppressed Ca^<2+> release from intracellular Ca^<2+> stores of pheochromocytoma cells (PC12 cells) by addition of caffeine or ATP.3. Addition of nerve growth factor (NGF) induced a neurite outgrowth of PC12 cells. In the presence of NGF, exposure to an extremely low frequency (ELF) magnetic field stimulate the differentiation.4. We measured the effects of ELF magnetic field on membrane structure of HeLa cells studied by Fourier transform infrared (FT-IR) spectroscopy. This exposure shifted the peak absorbance of the amide I band to a smaller wave number, reduced the peak absorbance of the amide II band, and increase absorbance at around 1600 cm^<-1>. These results suggest that the ELF field exposure has reversible effects on the N-H inplane bending and C-N stretching vibrations of peptide linkages, the changes the secondary structures of alpha-helix and beta-sheet in cell membrane proteins. We found similar changes in FT-IR spectra between cells exposed to ELF field and those incubated in an acidic medium, but these changes were different from those incubated in an high K^+ medium.

1. 时变1.5 T磁场不影响Ca^<2+>穿过牛肾上腺嗜铬细胞质膜的内流，但强烈抑制细胞内Ca^<2+>储存的Ca^<2+>释放由添加多种神经递质引起。 Ca ^ 2+ 储存通过兰尼碱受体释放的Ca ^ 2+ 也受到暴露的抑制。2．通过添加咖啡因或ATP，暴露于强磁场强烈抑制嗜铬细胞瘤细胞(PC12细胞)的细胞内Ca 2+ 储存的Ca 2+ 释放。3。添加神经生长因子 (NGF) 诱导 PC12 细胞的神经突生长。在存在 NGF 的情况下，暴露于极低频 (ELF) 磁场会刺激分化。4．我们通过傅里叶变换红外 (FT-IR) 光谱测量了 ELF 磁场对 HeLa 细胞膜结构的影响。该暴露使酰胺I带的峰值吸光度移动至更小的波数，降低了酰胺II带的峰值吸光度，并增加了1600cm ^ -1 附近的吸光度。这些结果表明，ELF场暴露对肽键的N-H面内弯曲和C-N伸缩振动具有可逆影响，从而改变细胞膜蛋白中α螺旋和β折叠的二级结构。我们发现暴露于 ELF 场的细胞和在酸性培养基中培养的细胞之间的 FT-IR 光谱有类似的变化，但这些变化与在高 K^+ 培养基中培养的细胞不同。