An attempt to establish a mouse line having a functional disorder in mast cells from wild mice.

尝试从野生小鼠中建立肥大细胞功能障碍的小鼠品系。

基本信息

批准号：
10680778
负责人：
TSUCHIYA Kimiyuki
金额：
$ 2.05万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Mus musculus manchu, a wild mouse, maintaining in Miyazaki Medical College was examined for checking an ability of worm expulsion from intestine against Strongyloides venezuelensis. A strain of S.venezuelensis, maintaining in the Laboratory of Veterinary Internal medicine, Miyazaki University were infected subcutaneously to the mice at a dose of 2000 L3. Number of the eggs in the feces were monitored 1, 2 and 3 weeks after infection to know the expulsive capacity of the mice. The expulsion of S.venezuelensis worms from the intestine of mice is known to be caused by effector mechanism (s) of mucosal mast cells, and was also proved that the number of worms remaining in the intestine parallels with egg counts. Mice having normal expulsive capacity could expel S.venezuelensis worms within 2-3 weeks of infection. In this experiment, 39 (43.3%) out of 90 M.m. manchu could expel worms within 3 weeks, so that they were decided to be grouped in the resistant (R) or normal. Twenty-six out of 90 (28.9%) could not expel the worms within 3 weeks, so that they were decided to be grouped in the susceptible (S) or defective.In the second experiment, we tried to have F1 from S x S and Rx Rto check further susceptibility of them. In the pairs of S x S, although we could not have enough number of F1 all mice showed S nature. All F1 from Rx Rwere R.For the establishment of conjenic mice which include S-gene, we are trying to have F1 from S x C57BL/6 mice.

对保存在宫崎医科大学的野生小鼠小家鼠(Mus musculus manchu)进行了检查，以检查其针对委内瑞拉类圆线虫的从肠内驱虫的能力。将宫崎大学兽医内科实验室保存的委内瑞拉沙门氏菌菌株以2000L3的剂量皮下感染小鼠。感染后1、2、3周监测粪便中虫卵数量，了解小鼠的排出能力。已知委内瑞拉链球菌蠕虫从小鼠肠道中排出是由粘膜肥大细胞的效应机制引起的，并且还证明残留在肠道中的蠕虫数量与虫卵数量平行。具有正常排出能力的小鼠可以在感染后2-3周内排出委内瑞拉沙门氏菌蠕虫。在本实验中，90 M.m. 中有 39 个（43.3%）。满洲人可以在3周内驱除蠕虫，因此将其分为抗性（R）或正常组。 90 只中有 26 只（28.9%）在 3 周内无法排出蠕虫，因此决定将它们归为易感（S）或缺陷。在第二个实验中，我们尝试从 S x S 中获得 F1和Rx R以进一步检查它们的敏感性。在S x S对中，尽管我们没有足够数量的F1，但所有小鼠都表现出S性质。所有来自Rx R的F1都是R。为了建立包含S基因的同系小鼠，我们正在尝试从S x C57BL/6小鼠中获得F1。