The search for the suitable species of bats to breed in the laboratory

寻找适合在实验室繁殖的蝙蝠品种

基本信息

批准号：
04558013
负责人：
TSUCHIYA Kimiyuki
金额：
$ 4.42万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

Tha aim of this project was to find out a certain species suitable to keep a bat for long period in the laboratory and to perform various experiments using the colony. The trial to keep suitable species of bats was tried by Tsuchiya, Kamada and Harada. Tsuchiya couldkeep Myotis macrodactylus and Miniopterus schreibersi for more than a year in the cage, and recorded the keeping condition. Harada examined 3 families 8 genera 10 speciea. He kept Murina leucogaster for more than 2 years, Plecotus auritus and Eptesicus japonensis for more than a year. Tsuchiya and Harada let the babies born from Pipistrellus abramus and Pteropus dasymallus mothers, respectively, who were already pregnant before capture, and raised them to adult in the laboratory. Tsuchiya and Kamada used meal worm as a food, wahile Harada compared silkworm, cricket and artificial food as food besides meal worm.Watanabe examined pituitary glands immunohistologically before and after capture. ACTH producing cell represented more prominent activity in captivity than before captivity, which means bats have stresses in laboratory condition. Gonadotropic cells were similar in both conditions. Kasai tried to induce artifical ovulation by PMSG+hCG in Miniopterus schreibersi with Tsuchiya and Kamada. The first with 2 individuals could not induce ovulation by 10 units dose of PMSG,while the second trial with 3 individuals given 2 and 3 times of dose seemed to induce ovulation by examining histology of ovary. More than one times of ovulation were suggested from the histological investigation. Matsunura studied frequency difference among various groups of Rhinolopus ferrumequinum. Kamada analyzed FM esolocation sounds of various species. Harada studied DNA systematics in bats.

该项目的目的是找出适合在实验室长期饲养蝙蝠的特定物种，并利用该群体进行各种实验。土屋、镰田和原田尝试过保留合适种类的蝙蝠。土屋将鼠耳蝠和小翅鼠在笼中饲养了一年多，并记录了饲养情况。原田检查了3科8属10种。他饲养了Murina leucogaster超过2年，Plecotus auritus和Eptesicus japonensis饲养了1年多。土屋和原田分别让在捕获前已经怀孕的黑翅狐蝠和大狐蝠母亲生下的婴儿，并在实验室中将它们抚养成人。 Tsuchiya和Kamada将粉虫作为食物，wahile Harada比较了蚕、蟋蟀和人工食物作为粉虫之外的食物。Watanabe在捕获前后对垂体进行了免疫组织学检查。产生 ACTH 的细胞在圈养中比圈养前表现出更显着的活性，这意味着蝙蝠在实验室条件下面临压力。两种情况下的促性腺细胞相似。 Kasai 尝试与 Tsuchiya 和 Kamada 一起通过 PMSG+hCG 在 Miniopterus schreibersi 中诱导人工排卵。第一个2人试验10单位剂量的PMSG不能诱导排卵，而第二个试验3个人给予2倍和3倍剂量，通过检查卵巢组织学似乎可以诱导排卵。组织学检查表明存在不止一次排卵。松浦研究了不同群体的马菊犀牛的频率差异。 Kamada 分析了不同物种的 FM 异位定位声音。原田研究了蝙蝠的 DNA 系统学。