Expression of the HSPB2 and αB-crystallin genes located in a head-to-head manner

HSPB2 和 αB-晶状体蛋白基因的表达以头对头的方式定位

• 批准号: 10680654
• 负责人: IWAKI Akiko
• 金额: $ 1.92万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680654/
• 关键词: Heat shock protein; Crystallin; Stress; ストレス蛋白質; クリスタン

Previously we identified a new member of the α-crystallin/small heat shock protein (HSP) family and named it HSPB2 (Genomics, 1997). The HSPB2 gene and the αB-crystallin gene, another member of the small HSP family, are arranged in a head-to-head manner with an intergenic sequence of 956 bp, raising a possibility of shared enhancer elements for their expression. In this study we examined spatial and temporal expression patterns of the newly identified HSPB2 gene and compared with that of the αB-crystallin gene. Furthermore, we investigated the regulatory mechanisms of expression of the two genes in cultured cells and transgenic mice. (1) Northern blotting and in situ hybridization revealed that the HSPB2 gene is expressed in heart and muscles but not in eye lens. Although the level of HSPB2 mRNA was much lower than that of αB-crystallin, the developmental profiles of their expression were similar in muscles (in preparation). (2) Functional promoter analysis of the two genes in C2C12 cells and transgenic mice revealed a competition of muscle specific enhancer elements, which arc present in the intergenic region (in preparation). (3) αB-Crystallin, but not HSPB2, accumulates in glial cells in response to high extracellular potassium concentrations. Functional promoter analysis using deletion and mutation constructs revealed that the heat shock element (HSE) is essential for transcriptional activation of the αB-crystallin gene by KCl in U-251MG.Gel mobility shift and antibody supershift assays showed that KCl induces the HSE-binding activity of heat shock factor 2 (HSF2), suggesting that HSF2 is involved in KCl-depedent increases in αB-crystallin mRNA in glial cells (submitted).

previouusly我们确定了α-晶状蛋白/小热休克蛋白（HSP）家族的新成员，并将其命名为HSPB2（Genomics，1997年）。用956 bp的inded空间和时间表达模式的前一基因序列与αB -crystalllin基因进行了比较，我们研究转基因。 C2C12细胞和转基因小鼠的基因启发了肌肉特异性元素的竞争，该元素存在于IAL中的ial细胞。 KCl在U-251MG.GEL迁移率转移和胫骨超速速测定中通过KCl转录激活αB-晶状体基因的必不可少的必不可少胶质细胞中αB-晶状蛋白mRNA的增加（提交）。

项目成果

Hamamura,M. et al.: "Differential decreases in c-fos and aldolase C mRNA expression in the rat cerebellum after repeated administration of methamphetamine"Mol. Brain.Res.. 64. 119-131 (1999)

滨村，M.

Sasaki, A., et al.: "Two autopsy cases with Pelizaeus-Merzbacher disease phenotype of adult onset, without mutation of proteolipid protein gene."Acta.Neuropathol.. 99. 7-13 (2000)

Sasaki, A., et al.：“两例尸检病例，具有成人发病的 Pelizaeus-Merzbacher 病表型，无蛋白脂质蛋白基因突变。”Acta.Neuropathol.. 99. 7-13 (2000)