RESEARCH ON alpha CRYSTALLIN DISTRIBUTED IN NON-LENTICULAR TISSUES

非晶状体组织中分布的α晶状体蛋白的研究

• 批准号: 03670518
• 负责人: KATO Kanefusa
• 金额: $ 1.34万
• 依托单位: INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURAL COLONY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670518/
• 关键词: alpha CRYSTALLIN; alphaB CRYSTALLIN; HEAT SHOCK PROTEIN; STRESS PROTEIN; IMMUNOASSAY; ALEXANDER'S DISEASE; クリスタリン; 培養細胞; アレキサンダ-病; 組織分布

1) Non-lenticular distribution of alphaA and alphaB crystallins was determined by means of newly established sensitive enzyme immunoassays. In contrast to the previous reports, it was found that not only alphaB crystallin, but also alphaA crystallin was present in non-lenticular tissues (spleen and thymus).2) During purification of alphaB crystallin from human skeletal muscle, we found that human small heat-shock protein, HSP28, was copurified with alphaB crystallin, and we showed evidences that indicate alphaB crystallin and HSP28 are associated in cells.3) Using specific immunoassays for alphaB crystallin and HSP28/27, responses to various types of stress of human and rat glioma cells were examined. It was found that alphaB crystallin responded to heat shock and chemical stressors similarly as did HSP28/27 in the same cells. These results indicate that alphaB crystallin is a member of small HSPs.4) The responses of the two proteins (alphaB and HSP27) in rat tissues to heat shock subjected in vivo were examined. The responses of alphaB crystallin were less sensitive than those of HSP27 in most of rat tissues including central nervous tissues.5) We clarified that the expression of mRNA for alphaB crystallin in the brain of a patient with Alexander's disease was enhanced, and found that concentrations of alphaB crystallin and HSP28 in several samples of cerebrospinal fluid of the patients were significantly enhanced.

1）通过新建立的敏感酶免疫测定的方式确定αA和α晶体的非末端分布。与先前的报道相反，发现不仅α晶体蛋白，而且还存在αA晶体蛋白（脾脏组织（脾和胸腺）。2）.2）。热休克蛋白Hsp28与Alphab Crystallin共同纯化，我们表明表明Alphab Crystallin和Hsp28的证据与细胞中有关。3）使用特定的Alphab Crystallin和Hsp28/27的免疫测定，对人类和人类和人类的各种应力的反应检查了大鼠神经胶质瘤细胞。已经发现，α晶体素对热休克和化学胁迫的反应与同一细胞中HSP28/27一样。这些结果表明，对大鼠组织中两种蛋白（alphab和hsp27）对体内热休克的反应（Alphab和Hsp27）的反应是小HSP的成员。在包括中枢神经组织在内的大多数大鼠组织中，α晶体蛋白的反应不如HSP27的敏感性。5）我们澄清说，在亚历山大病患者的大脑中，alphab晶体蛋白的mRNA表达增强了，并发现浓度是在几种患者脑脊液样品中，α晶体和HSP28的含量显着增强。

项目成果

Inaguma,Y.,Kato,K.,et al.: "Immunoreactive parvalbumin concentrations in parahippocampal gyrus decrease in patients with Alzheimers disease." J.Neurol.Sci.110. 57-61 (1992)

Inaguma, Y., Kato, K., et al.：“阿尔茨海默病患者海马旁回的免疫反应性小白蛋白浓度降低。”

Asano,T.,Kato,K.,et al.: "Two forms of Go type G proteins:Identification and distribution in various rat tissues and cloned cells." J.Neurochem.58. 2176-2181 (1992)

Asano,T.,Kato,K.,et al.：“Go 型 G 蛋白的两种形式：在各种大鼠组织和克隆细胞中的鉴定和分布。”

HARUO SHINOHARA: "αB crystallin and 28-kDa heat shock protein are enhanced in the cerebral cortex of Alzheimer's disease."

Haruo Shinohara：“αB 晶状体蛋白和 28-kDa 热休克蛋白在阿尔茨海默病的大脑皮层中得到增强。”

Inaguma,Y.,& Kato,K.,et al.: "Translocation And induction of αB crystallin by heat shock in rat glioma (GA-1) cells." Biochem.Biopys.Res.Commun.182. 844-850 (1992)

Inaguma, Y. 和 Kato, K. 等人：“大鼠神经胶质瘤 (GA-1) 细胞中热休克对 αB 晶状体蛋白的易位和诱导。”Biochem.Biopys.Res.Commun.182（ 1992）

Ochi,N., Kobayashi,K., Maehara,M., Nakayama,A., Negoro,T., Shinohara,H., Watanabe,K., Nagatsu,T. and Kato,K: "Inceament of alphaB-crystallin mRNA in the brain of patient with infantile type Alexander's disease" Biochem. Biophys. Res. Commun. 179. 1030-103

大智，N.，小林，K.，前原，M.，中山，A.，根戈罗，T.，筱原，H.，渡边，K.，长津，T.