Basic studies on possible application of anti-oxidative fraction and constituents of crude drug origin to preventive medicine

抗氧化部位及药材来源成分在预防医学中的可能应用基础研究

基本信息

批准号：
10671998
负责人：
KINOSHITA Takeshi
金额：
$ 0.64万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Chemical investigation was carried out intensively on the methylene chloride extract (PT ext.) of Glycyrrhiza glabra root (Russian licorice) to afford four minor phenolic constituents, two isoflavanones (RL-Q and RL-R), one 3-arylcoumarine (RL-U) and one chromenochromane (RL-V), all of which were undescribed literally, Structures of these compounds were elucidated as 2',4'-dihydroxy-[6",6"-dimethylpyrano(2",3":7,8)]-isoflavanone, [6",6"-dimethylpyrno(2",3":7,8)]-2'-hydroxy,4'-methoxy-isoflavanone, 4',7-dihydroxy-[6",6"-dimethylpyno(2",3":2',3')]-3-arylcoumarin [6',6' -dimethylpyrano(2', 3' : 7, 8)]-benzopyran-3-ol, respectively, on the spectroscopic basis.2. Constituents obtained from Russian licorice described above were tested for anti-microbial activity against gram-positive bacteria including MRSA, yeasts, moulds, and so on. Some of phenolic constituents of this licorice showed potent activity against wide variety of microorganisms.3. The above constituents were assayed for anti … More -oxidative activity on linoleic acid, scavenging activity of superoxide anion (SOィイD3-(/)2ィエD3) generated in both xanthine-xanthine oxidase and microsomal systems, and preventing activity of lipid peroxidation in both microsomal and mitochondrial systems. Many of licorice constituents exhibited outstanding anti-oxidative activity in various assay systems indicating that licorice extract (PT ext.) is a promising agent for the prevention of disease in which active oxygen species are suspected to be implicated on the in-vitro basis. It is of particular interest to note that RL-M showed the most potent anti-oxidative activity among licorice constituents tested though it does not possess catechol moiety that is fond very commonly in naturally occurring antioxidants, Licorice extract also showed significant inhibitory activity against oxidative hemolysis of human erythrocytes, but this assay failed to assess anti-oxidative hemolysis activity of purified constituents due to their direct action to erythrocyte membrane. Less

1。化学研究是在甘氨酸glabra glabra root（俄罗斯持牌液）的二氯化氯化物提取物（Pt Ext。 Structures of these compounds were elucidated as 2',4'-dihydroxy-[6",6"-dimethylpyrano(2",3":7,8)]-isoflavanone, [6",6"-dimethylpyrno(2",3":7,8)]-2'-hydroxy,4'-methoxy-isoflavanone, 4',7-dihydroxy-[6",6"-dimethylpyno(2",3":2',3')]-3-arylcoumarin [6',6' -dimethylpyrano(2', 3' :7, 8)]-benzopyran-3-ol, respectively, on the spectroscopic basis.2.测试了从上述俄罗斯甘草中获得的成分，对革兰氏阳性细菌的抗微生物活性进行了测试，包括MRSA，酵母，霉菌等。该甘草的某些酚类成分显示了对各种微生物的潜在活性。3。上述成分被分配用于抗…对亚油酸的氧化活性，超氧阴离子的清除活性（SOII D3 - （/）2IE D3）在木胺 - Xanthine氧化酶和微粒细胞系统中产生的氧化活性（SOII D3 - （/）2IE D3），以及在微粒体和MITOCOCHONDRILAL SYSTEMS中脂质过氧化的活性。许多甘草构成在各种测定系统中表现出杰出的抗氧化活性，表明甘草提取物（Pt Ext。）是预防疾病的承诺药物，其中怀疑是在体外实施活性氧。特别值得注意的是，RL-M在甘草中表现出最有效的抗氧化活性，构成了测试的最有效的抗氧化活性，尽管它不假定在自然发生的抗氧化剂中非常喜欢的Catechol部分，但甘草提取物在自然发生的抗氧化剂中也非常喜欢，但该抗氧化性抑制作用也表现出对人类突变性的氧化性溶液的氧化性溶液的氧化性溶液，但其施用量强度的抗氧化作用，抗氧化型的抗氧化作用，抗氧化型的抗氧化作用均具有抗氧化作用，而抗氧化型的抗氧化作用是抗氧化剂氧化的氧化作用。红细胞膜。