Basic studies on possible application of anti-oxidative fraction and constituents of crude drug origin to preventive medicine

抗氧化部位及药材来源成分在预防医学中的可能应用基础研究

基本信息

批准号：
10671998
负责人：
KINOSHITA Takeshi
金额：
$ 0.64万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Chemical investigation was carried out intensively on the methylene chloride extract (PT ext.) of Glycyrrhiza glabra root (Russian licorice) to afford four minor phenolic constituents, two isoflavanones (RL-Q and RL-R), one 3-arylcoumarine (RL-U) and one chromenochromane (RL-V), all of which were undescribed literally, Structures of these compounds were elucidated as 2',4'-dihydroxy-[6",6"-dimethylpyrano(2",3":7,8)]-isoflavanone, [6",6"-dimethylpyrno(2",3":7,8)]-2'-hydroxy,4'-methoxy-isoflavanone, 4',7-dihydroxy-[6",6"-dimethylpyno(2",3":2',3')]-3-arylcoumarin [6',6' -dimethylpyrano(2', 3' : 7, 8)]-benzopyran-3-ol, respectively, on the spectroscopic basis.2. Constituents obtained from Russian licorice described above were tested for anti-microbial activity against gram-positive bacteria including MRSA, yeasts, moulds, and so on. Some of phenolic constituents of this licorice showed potent activity against wide variety of microorganisms.3. The above constituents were assayed for anti … More -oxidative activity on linoleic acid, scavenging activity of superoxide anion (SOィイD3-(/)2ィエD3) generated in both xanthine-xanthine oxidase and microsomal systems, and preventing activity of lipid peroxidation in both microsomal and mitochondrial systems. Many of licorice constituents exhibited outstanding anti-oxidative activity in various assay systems indicating that licorice extract (PT ext.) is a promising agent for the prevention of disease in which active oxygen species are suspected to be implicated on the in-vitro basis. It is of particular interest to note that RL-M showed the most potent anti-oxidative activity among licorice constituents tested though it does not possess catechol moiety that is fond very commonly in naturally occurring antioxidants, Licorice extract also showed significant inhibitory activity against oxidative hemolysis of human erythrocytes, but this assay failed to assess anti-oxidative hemolysis activity of purified constituents due to their direct action to erythrocyte membrane. Less

1. 对光果甘草根（俄罗斯甘草）的二氯甲烷提取物（PT ext.）进行了深入的化学研究，得到了四种微量酚类成分、两种异黄酮（RL-Q 和 RL-R）、一种 3-芳基香豆素（ RL-U）和一种色并色烷（RL-V），所有这些化合物都没有字面描述，这些化合物的结构是阐明为 2',4'-二羟基-[6",6"-二甲基吡喃(2",3":7,8)]-异黄烷酮，[6",6"-二甲基吡喃(2",3":7, 8)]-2'-羟基，4'-甲氧基-异黄烷酮， 4',7-二羟基-[6",6"-二甲基吡喃(2",3":2',3')]-3-芳基香豆素[6',6'-二甲基吡喃(2',3':7, 8)]-苯并吡喃-3-醇，分别基于光谱。2.测试了上述从俄罗斯甘草中获得的成分对革兰氏阳性菌的抗菌活性。甘草中的一些酚类成分对多种微生物表现出有效的活性。3.对上述成分进行了亚油酸的抗氧化活性、超氧阴离子的清除活性的测定。 (SOiiD3-(/)2ieD3) 在黄嘌呤-黄嘌呤氧化酶和微粒体系统中产生，并防止脂质活性微粒体和线粒体系统中的许多突出的甘草成分在各种测定系统中都显示出抗氧化活性，这表明甘草提取物（PT ext.）是一种有前途的预防活性氧类疾病的药物。值得注意的是，在所测试的甘草成分中，RL-M 显示出最有效的抗氧化活性，尽管它不具有儿茶酚部分。甘草提取物在天然存在的抗氧化剂中非常常见，它还对人类红细胞的氧化溶血表现出显着的抑制活性，但由于其对红细胞膜的直接作用较少，因此该测定未能评估纯化成分的抗氧化溶血活性。