Analysis for Prostaglandin E2 and Vascular Endothelial Growth Factor in the Articular Disc and Synovial Cell in case of Internalderangement of the Temporomandibular Joint

颞下颌关节内部紊乱时关节盘和滑膜细胞中前列腺素 E2 和血管内皮生长因子的分析

• 批准号: 10671872
• 负责人: FUJITA Shigeyuki
• 金额: $ 2.43万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671872/
• 关键词: VEGF; COX-1; Substance-P; Synovial membrane; Prostaglandin E; Immunohisto chemistry; Temporomandibulac joint; Synovial cell; Immunchistochemistry; Temporomandibular Joint; Synovial cell; Prosta glandin E_2; シクロオキシゲナーゼ-1; シクロオキシゲナーゼ-2; 顎関節内障

In Anatomy institute, Tubingen university Germany, I researched the relation between the variation of the path way on lateral-pterygoid muscle and deformity of the articulator-disc, as the result of this research, there was no significance between them. But in the completely deformed articulator disc, high concentration of keratan-sulfate and chondroitin-6 sulfate wereidentified in the antero-inferior part of the disc and hyperplastic synovial membrane was identified in these cases.In the completely deformed articulator disc of clicical internalderangement cases, Tenascin was recognized in the hyperplastic synovial membrane and Matrix Metaro Protease-3 also found in the disc, which means remodeling and reproduction were occurred in the same time. In such cases, Substance-P and COX-1 were distributed near the vascular vessels in the hyperplastic synovial membrane, and Localization of Heat-Schock Protein-47 (HSP-47) was also specific in the antero-inner part of the disc, but the HSC-70 was identified in roard bearing part of the disc. We also established the synovial cell line from internalderangement cases, with the stimulation of Inter-leukin-1b (1ng/ml), the cell line autocrined Vascular

在德国蒂宾根大学解剖研究所，我研究了翼外肌路径的变化与咬合盘畸形之间的关系，该研究的结果是，两者之间没有显着性。但在完全变形的咬合盘中，在盘的前下部发现了高​​浓度的硫酸角质素和硫酸软骨素，并且在这些病例中发现了增生的滑膜。在临床内部紊乱病例的完全变形的咬合盘中，在增生的滑膜中发现了腱蛋白，在椎间盘中也发现了Matrix Metaro Protease-3，这意味着发生了重塑和复制在同一时间。在这种情况下，P物质和COX-1分布在增生滑膜的血管附近，热休克蛋白47（HSP-47）的定位也特异于椎间盘的前内侧部分，但在制动盘的道路轴承部分发现了 HSC-70。我们还从内部紊乱病例中建立了滑膜细胞系，并用白细胞介素-1b (1ng/ml) 刺激，该细胞系自分泌血管

项目成果

Hiroaki Yoshida: "The distribution of cycloxygenase-1 in human Temporomandibular joint samples"Journal of Oral Rehabilitation. (In press). (1999)

Hiroaki Yoshida：“环氧化酶-1在人类颞下颌关节样本中的分布”口腔康复杂志。

Hiroaki Yoshida: "The localization of Matrix Metalloproteinase-3 and Tenascin in synovial membrane of the temporomandibula joint"Oral Disease. 5. 50-54 (1999)

Hiroaki Yoshida：“基质金属蛋白酶-3 和腱蛋白在颞下颌关节滑液膜中的定位”口腔疾病。

Hiroaki Yoshida: "Angiogenesis in the human temporomandibular Joint studied by immunohistochemistry for CD34 antigen"Journal of Oral Pathology and Oral Medicine. 28. 289-292 (1999)

Hiroaki Yoshida：“通过 CD34 抗原的免疫组织化学研究人颞下颌关节的血管生成”口腔病理学和口腔医学杂志。

Yoshida H, Fujita S.Yoshida T.Iizuka T, Sakakura T: "Angiogenesis in human temporomandibular joint studied by immunohistochemistry for CD34 antigen"Journal of Oral Pathology and Oral Medicine. 28. 289-292 (1999)

Yoshida H、Fujita S.Yoshida T.Iizuka T、Sakakura T：“通过 CD34 抗原的免疫组织化学研究人颞下颌关节的血管生成”口腔病理学和口腔医学杂志。