Expression of endomcrphine along pain puthuay in experimental peripmend neuropathy in rat

大鼠实验性周围神经病变中内啡肽沿痛觉的表达

基本信息

批准号：
10671412
负责人：
SHIBATA Masahiko
金额：
$ 2.11万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671412/
关键词：
neuropathic pain rat lithium nerve stimulation halothane 痛みノチセプチン神経因性疼病 endomorphin

项目摘要

After peripheral nerve injury, patients sometimes suffer from refractory pain, such as post herpetic neuralgia and causalgia Non-steroidal anti-inflammatory drugs or opioid are not effective for such condition. We, using sciatic nerve injury model in rat, showed that electro-convulsive treatment and intrathecal administration of lithium are effective for pain after nerve injury. These studies suggest that central nervotus system relating affective component can be a primary target for treating neuropathic pain.At first stage in this study, we planned to investigate about change of endomorphine and nociceptinalong pain transmitting tract by using immuno-cytochemistry technique because we speculated that poor effect of opioid for neuropathic pain might be due to changes in these opioid system. However antibody for endomorphine and nociceptin did not work well, so, we changed the protocol of this study. In this study we investigated about mechanism of lithium on pain relieving effect after nerve injury by co-administrating myo-inositol. Intrathecal co-administration of myo-inositol reversed the effect of lithitum on pain after nerve injury, which suggests that second messenger system had important role for lithium effect on pain after nerve injury. We also examined whether repetitive stimulation to injured nerve under halothane anesthesia could relieve pain after nerve injury. We also showed that repetitive stimulation to injured nerve under halothane anesthesia could relieve pain after nerve injury, suggesting long term depression may contribute for pain relief after nerve injury. These results encourage the new therapetttic approach for neuropathic pain.

周围神经损伤后，患者有时会出现顽固性疼痛，如带状疱疹后神经痛、灼痛等，非甾体类抗炎药或阿片类药物治疗效果不佳。我们使用大鼠坐骨神经损伤模型表明，电休克治疗和鞘内注射锂对神经损伤后的疼痛有效。这些研究表明，与情感成分相关的中枢神经系统可以成为治疗神经性疼痛的主要靶点。在本研究的第一阶段，我们计划利用免疫细胞化学技术研究内吗啡和伤害感受器沿疼痛传导路的变化，因为我们推测：阿片类药物治疗神经性疼痛的效果不佳可能是由于这些阿片类药物系统的变化所致。然而，内吗啡和伤害感受肽的抗体效果不佳，因此，我们改变了本研究的方案。在本研究中，我们研究了锂与肌醇联合给药对神经损伤后疼痛缓解作用的机制。鞘内联合给予肌醇逆转了锂对神经损伤后疼痛的作用，这表明第二信使系统在锂对神经损伤后疼痛的作用中起重要作用。我们还研究了在氟烷麻醉下对受伤神经进行重复刺激是否可以缓解神经损伤后的疼痛。我们还表明，在氟烷麻醉下重复刺激受损神经可以缓解神经损伤后的疼痛，这表明长期抑郁可能有助于缓解神经损伤后的疼痛。这些结果鼓励了神经性疼痛的新治疗方法。