REGULATION OF STRESS S-RELATED GENE EXPRESSION IN THE STOMACH

胃中应激 S 相关基因表达的调节

基本信息

批准号：
10670479
负责人：
ROKUTAN Kazuhito
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670479/
关键词：
STRESS HEAT SHOCK PROTEIN 70 ADRENAL GLANDS CATECHOLAMINES GLUCOCORTICOIDS STRESS ULCER ADRENALECTOMY VAGOTOMY Heat shock protein 70 ストレス反応ストレス性胃粘膜障害熱ショック蛋白質生体内シシグナルストレスホルモン

项目摘要

Restraint and water-immersion stress rapidly induces heat shock protein 70 (HSP70) in rat gastric mucosa. When bilaterally adrenalectomized or subdiaphragmatically vagotomized rats were exposed to the stress, adrenalectomy completely abolished the stress-induced activation of heat shock factor 1 (HSF1). HSP70 mRNA expression, and HSP70 induction, while vagotomy enhanced them. These surgical manupulations did not change the HSF1 level in gastric mucosa. The extent of-the HSP70 induction correlated inversely with the severity of mucosal damage, suggesting an important role of HSP70 in the defense against stress ulcer. Intraperitoneal injection of epinephrine, but not norepinephrine nor dexamethasone, could induce HSP70 in the gastric mucosa of adrenalectomized rats. The stress-induced HSP70 induction in normal rats was inhibited by αィイD21ィエD2- and αィイD21AィエD2-adrenoceptor antagonists, leading to aggravate mucosal damage, while αィイD21B/1DィエD2-, αィイD22ィエD2- and β-adrenoceptor antagonists or a glucocorticoid receptor antagonist had no effect on the HSP70 induction and mucosal damage. Our results suggest that the stress-induced heat shock response of gastric mucosa may be under adrenergic regulation, possibly through the αィイD21AィエD2- adrenergic receptor.

束缚和水浸应激会在大鼠胃粘膜中迅速诱导热休克蛋白 70 (HSP70)。当双侧肾上腺切除或膈下迷走神经切断的大鼠暴露于应激时，肾上腺切除术完全消除了应激诱导的热休克因子 1 (HSF1) 激活。 HSP70 mRNA 表达和 HSP70 诱导，而迷走神经切断术增强了它们。这些手术操作并没有改变 HSF1。胃粘膜中 HSP70 的诱导程度与粘膜损伤的严重程度呈负相关，这表明 HSP70 在防御应激性溃疡中发挥着重要作用，但腹腔注射肾上腺素（而非去甲肾上腺素或地塞米松）可在胃粘膜中诱导 HSP70。肾上腺切除大鼠的胃粘膜正常大鼠应激诱导的 HSP70 诱导受到抑制。 αD21D2-和αD21AD2-肾上腺素受体拮抗剂，导致加重粘膜损伤，而αD21B/1D2-、αD22D2-和β-肾上腺素受体拮抗剂或糖皮质激素受体拮抗剂对HSP70诱导和粘膜损伤没有影响。感应热胃粘膜的休克反应可能受到肾上腺素能调节，可能通过α-D21A-肾上腺素能受体。