Comparative ecological study of hantavirus in rodents in Japan and Far East Russia

日本和俄罗斯远东地区啮齿类动物汉坦病毒的比较生态学研究

基本信息

批准号：
10660296
负责人：
KARIWA Hiroaki
金额：
$ 2.3万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660296/
关键词：
Hantavirus Hemorrhagic fever Rodent Emerging infection Zoonosis

项目摘要

Epidemiological study was conducted in Japan and Far East Russia and the followings were revealed.1. Hantavirus was identified in the urine from infected rats. Since newborn rats intranasally inoculated with the urine containing virus had the virus in their organs and urine, the virus may be maintained by the transmission with virus-contaminated urine by intranasal route in nature.2. Enzyme linked immunosorbent assay (ELISA) using baculovirus-expressed nucleocapsid proteins of different hantaviruses as antigens was established. This assay made it possible to detect hantaviral antibody and also identify the type of infected hantavirus when sera were reacted with different antigens.3. To evaluate the virus load in patients of hantavirus pulmonary syndrome (HPS), quantitative PCR system was established. According this PCR, it was revealed that high amount of virus was detectable in blood of acute HPS patients but the virus was disappeared form blood in convalescent phase.4. Large scale of epidemiological study of hantavirus was conducted in Japan by using severa serological methods. In healthy adults, no one had hantaviral antibody while in hepatic disease patients of unknown etiology, 2 to 3 % of cases had the antibodies. Therefore, it is no doubt that the general public in Japan have hantavirus infections.5. Epizootiologic study of hantavirus targeting for rodents was carried out in Vladivostok, Far East Russia. Hantaviral antibody was detected in gray-backed vole (Clethrionomys rufocanus), hich is known to be a reservoir of Puumala type hantavirus in Hokkaido, Japan. Since stripped field mice (Apodemus agrarius), which is a reservoir of Hantaan type hantavirus in China and Korea, also had the antibodies, highly virulent strain may be prevalent in Far Eastern Russia. In addition, hantavirus genome was identified in reed vole (Microtis fortis) and sequence analysis revealed that this virus was distinct from other known hantaviruses.

流行病学研究在日本和远俄罗斯进行了，并揭示了以下内容。1。在感染大鼠的尿液中鉴定出汉坦病毒。由于新生大鼠与含有尿液的尿液的鼻腔内接种，其器官和尿液中有病毒，因此该病毒可以通过自然界内鼻内途径通过鼻内途径的病毒污染的尿液来维持该病毒。2。酶使用抗原作为抗原的不同汉坦病毒的杆菌病毒表达的核蛋白蛋白连接了免疫吸附测定法（ELISA）。该测定法使检测汉坦病毒抗体并确定血清与不同抗原反应时感染的汉坦病毒的类型。3。为了评估汉塔病毒肺综合征（HPS）患者的病毒载荷，建立了定量PCR系统。根据这一PCR，据显示，急性HPS患者的血液中可检测到大量病毒，但该病毒在疗养期消失了血液。4。通过使用Severa血清学方法，在日本进行了大规模的汉塔病毒流行病学研究。在健康的成年人中，没有人患有汉坦病毒抗体，而在肝病患者中，有2％至3％的病例患有抗体。因此，毫无疑问，日本的普通公众感染了汉坦病毒感染5。汉塔病毒靶向啮齿动物的epizootiologic研究在北俄罗斯远东的弗拉基维斯托克进行了。汉坦病毒抗体是在日本北海道的Puumala Type Hantavirus的水库中检测到的。由于被剥离的野外小鼠（Apodemus Agrarius）是中国和韩国汉塔型汉塔病毒的水库，也具有抗体，因此在俄罗斯远东地区可能会普遍存在。此外，在芦苇沃尔（Microtis Fortis）中鉴定出汉塔病毒基因组，序列分析表明该病毒与其他已知的汉塔病毒不同。