Elucidation of immnoregulating function of casein phosphopeptide

酪蛋白磷酸肽免疫调节功能的阐明

• 批准号: 10660258
• 负责人: OTANI Hajime
• 金额: $ 2.3万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660258/
• 关键词: Milk casein; Casein phosphopeptides; Immunoregulator; Cytokines; Intestinal IgA; Mitogenic activity; Oral administration; Lymphocytes; 経口投与試験; 機能性食品事材; カゼイン; 免疫賦活; IgA; 経口投与; 豚; 機能性飼料; 牛乳; 免疫グロブリン生産; リンパ球増殖; マイトージェン活性; 特定保健用食品

An immunomodulating peptide was purified from a trypsin digest of bovine αs1-casein and identified as residues 59-79 in bovine αs1-casein. The sequence 59-79 has a Phosphoserine-rich region and is called one of casein phosphopeptides. Bovine β-casein aloso possesses phosphoserin-rich region. Hence, the author isolated β-casein fragment 1-25, one of the casein phosphopeptides, from tryptic β-casein and confirmed that the phosphopeptide displayed the immunomodulating action as well as the casein phosphopeptide from bovine αs1-casein.The casein phosphopeptides displayed a mitogenic activity and stimulated the mitogen-induced proliferations and the immunoglobulin production via the stimulation of IL-5 and IL-6 produced from Th2 cells in mouse spleen cell cultures. The oral adminstration of casein phosphopeptides enhanced the levels of antigen-specific IgA and total IgA in mice and piglets. Moreover, the immunostimulating activity was confirmed to a phosphopeptide having at least 2 phosphoserine residues, i.e., SerP-Leu-SerP.It has been widely reported that various kinds of casein phosphopeptides are produced from bovine caseins by the action of various kinds of proteolytic enzymes. Thus, the casein phosphopeptides prepared from bovine casein digests are beneficial foodstuffs for the enhancement of mucosal IgA responses in man and livestock.

从牛αS1-casen的胰蛋白酶消化物中纯化了免疫调节的肽，并在牛αS1-Casein E 59-79中被鉴定为残基59-79，称为富含磷酸盐的区域，被称为蛋白蛋白磷酸肽。富含磷酸蛋白的区域，并确认硫蛋白菌显示了牛的免疫模板调节以及硫哌迪酯。在小鼠和小猪中。为了增强人和牲畜中粘膜IgA反应。

项目成果

M. A. Matin & H. Otani: "Release of cytotoxic glycopeptides from human acid casein fraction by the action of stomach proteinases"Milchwissenschaft. 55(1). 6-10 (2000)

M.A.马丁

Hajime Otani: "Current Research of Advanced Series in Agricultural & Biological Chemistry"World Wide Research Network Publisher(in press). (2001)

Hajime Otani：“农业先进系列研究现状

Hajime Otani, Yukihiro Kihara and Minkyu Park: "The immunoenhancing property of a dietary casein phosphopeptide preparation in mice"Food and Agricultural Immunology. 12. 165-173 (2000)

Hajime Otani、Yukihiro Kihara 和 Minkyu Park：“小鼠膳食酪蛋白磷酸肽制剂的免疫增强特性”食品和农业免疫学。

I.Hata,S.Higashiyama & H.Otani: "Identification of a phosphopeptide in bovine αs1-casein digest as a factor influencing proliferation and immunogloblin production in lymphocyte cultures"Journal of Dairy Research. 65. 569-578 (1998)

I.Hata、S.Higashiyama 和 H.Otani：“牛 αs1-酪蛋白消化物中磷酸肽作为影响淋巴细胞培养物增殖和免疫球蛋白产生的因素的鉴定”《乳制品研究杂志》65. 569-578 (1998)。

H.Otani,H.Kitamura et al.,: "Enhancement of intestinal IgA levels in piglets by oral administration of a commercially available casein phosphopeptide preparation"Milchwissenschaft. 55. 429-432 (2000)

H.Otani、H.Kitamura 等人：“通过口服市售酪蛋白磷酸肽制剂提高仔猪肠道 IgA 水平”Milchwissenschaft。