Confocal Microscope for Three-Dimensional Polarization Analysis and Its applications to biology

三维偏振分析共焦显微镜及其在生物学中的应用

• 批准号: 10650041
• 负责人: KAWATA Yoshimasa
• 金额: $ 2.3万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650041/
• 关键词: microscopy; confocal microscopy; polarization microscopy; laser; polarization anlaysis; sigle molecular; biology; optical elasticity; microscopy; confocal microscopy; polarization microscopy; laser; polarization anlaysis; sigle molecular; biology; optical elasticity

A confocal microscope for the use of three-dimensional polarization analysis was developed. Since the pinhole before the detector eliminates the light whose polarization is rotated by an imaging lens, the polarization properties of specimens are observed with high contrast. The pinhole size required to achieve high contrast was discussed. Three-dimensional resolution of the polarization microscope was also demonstrated.The three-dimensional imaging analysis using the extended Mie scattering theory was developed. In the analysis, we calculated the images of a Mie particle whose diameter is comparable with the wavelength of confocal microscope. It was found that, when we observe a Mie particle, polarization confocal microscopy is not affected by the polarization distortion that is due to focusing with high-numerical-aperture lenses and does not produce pseudopeaks in the images in comparison with conventional polarization microscopy. The three-dimensional resolution of the polarization microscope and the verification of the proposed analysis method were also discussed.

开发了用于使用三维极化分析的共聚焦显微镜。由于检测器前的针孔消除了通过成像透镜旋转极化的光，因此观察到标本的极化特性，并具有很高的对比度。讨论了实现高对比度所需的针孔尺寸。还展示了极化显微镜的三维分辨率。使用扩展的MIE散射理论进行了三维成像分析。在分析中，我们计算了直径与共聚焦显微镜波长相当的MIE粒子的图像。发现，当我们观察到MIE粒子时，极化共焦显微镜不受极化失真的影响，而极化失真是由于聚焦在高数字驱动器镜片上，并且与常规极化显微镜相比，图像中不会在图像中产生假峰。还讨论了极化显微镜的三维分辨率和提出的分析方法的验证。