Mous Saturation mutagenesis project:Construction of sperm bank for chromosome deletion mutants and development of screening system for the mutations

小鼠饱和诱变项目：染色体缺失突变体精子库建设及突变筛选系统开发

• 批准号: 10358019
• 负责人: SHIROISHI Toshihiko
• 金额: $ 26.24万
• 依托单位: Natiomal Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10358019/
• 关键词: Chromosome deletion; Japanese wild mouse; chlorambucil; Rapid blood clotting; 精子凍結; 低血糖傾向; RLGSマッピング; マイクロサテライトマーカー

We carried out in-depth phenotype characterization of mouse mutants identified in screening for chlorambucil (CHL) treated Gl mice. The mutants that we characterized were ; strain ID numbers, CHL0106 and CHL0225. The former mutant showed lower glucose level in serum, and the latter showed rapid blood clotting. Inheritance of these two mutants was confirmed by cross of the mutants with wild-type mice. For the CHL0225, we analyzed platelet coagulation and counted the number of the platelets in order to examine abnormalities of the function and the amount of the platelets. In addition, we assayed activated thromboplastin time, prothrombin time. Moreover, Amount of fibrimonomer complex antithromobin-thromobin complex was measured in order to examine the function of anti-coagulation factors. As a result, we could not find abnormalities in the all parameters of the CHL0225. It indicated that the mutant CHL0225 is not affected in the known coagulation factors, the anti-coagulation factors and the platelet function, but rather unknown gene in the blood clotting cascade is mutated.

我们对在筛选苯丁酸氮芥(CHL)处理的G1小鼠中鉴定的小鼠突变体进行了深入的表型表征。我们表征的突变体是；菌株 ID 号：CHL0106 和 CHL0225。前一种突变体表现出血清中较低的葡萄糖水平，而后者则表现出快速的血液凝固。通过突变体与野生型小鼠的杂交证实了这两个突变体的遗传。对于CHL0225，我们分析血小板凝固并计数血小板数量，以检查血小板功能和数量的异常。此外，我们还测定了活化凝血活酶时间、凝血酶原时间。此外，为了检查抗凝血因子的功能，测定纤维单体复合物抗凝血酶-凝血酶复合物的量。结果，我们没有发现CHL0225的所有参数有异常。表明突变体CHL0225在已知的凝血因子、抗凝因子和血小板功能上没有受到影响，而是在凝血级联中的未知基因发生了突变。

项目成果

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Shimada Y. 等人：“小鼠胸腺淋巴瘤中 11 号染色体上 Znfn1a1(Ikaros) 基因座的杂合性与辐射相关的损失。”Radiat.Res.. 154. 293-300 (2000)

Ueda, Y. et al.: "Nucleotide sequences of the mouse globin beta cDNAs in a wild derived new haplotype Hbbwl"Mammal. Genome. 10. 879-882 (1999)

Ueda, Y. 等人：“野生衍生的新单倍型 Hbbwl 中小鼠珠蛋白 β cDNA 的核苷酸序列”哺乳动物。

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Nagase, H. 等人：“分泌可溶性 CD4 而不表达膜结合 CD4 的新型突变小鼠。”

Hattori, M. et al.: "Cutting edge : homelogous recombination of the MHC class I K region defines new MHC-Linked diabetogenic susceptibility gene(s) in nonobese diabetic mice"J. Immunol. 163. 1721-1724 (1999)

Hattori, M. 等人：“前沿：MHC I 类 K 区域的同源重组定义了非肥胖糖尿病小鼠中新的 MHC 相关糖尿病易感基因”J.

Komine, Y. et al.: "A novel type of non-coding RNA expressed in the rat brain"Mol. Brain Res.. 66. 1-13 (1999)

Komine, Y. 等人：“在大鼠大脑中表达的一种新型非编码 RNA”Mol.