Molecular mechanism of vascular system formation

血管系统形成的分子机制

基本信息

批准号：
10304063
负责人：
FUKUDA Hiroo
金额：
$ 23.87万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

1. Analysis with Arabidopsis vascular mutants, van1-van7 : Molecular analysis of Arabidopsis vascular mutants, van1-van7 with a pAthb8-GUS or pTED3-GUS fusion gene revealed that defects of these mutants were caused by abnormal pattern formation of procambial cells. In addition, we displayed that the continuity of the vascular network is more sensitive to genetic lesions than their overall architecture is, supporting the diffusion-reaction prepattern model.2. Analysis of the HD-Zip homeobox genes during carrot somatic embryogenesis. We analyzed expression pattern of 6 HD-Zip homeobox genes, CHB1-CHB6, with in situ hybridization during carrot somatic embryogenesis, and revealed that all the genes were expressed in association with vascular cells in the late stage of embryogesesis, that is, CHB6 was expressed specifically in procambium and others were in different vascular cells. These results suggest that the HD-Zip homeobox genes are involved in vascular formation.3. Analysis of vascula … More r cell differentiation with an in vitro Zinnia system in which mesophyll cells differentiate into vascular cells.(1) We revealed the presence of different kind of vascular cells in the in vitro Zinnia culture system.(2) We established new culture methods, with which a tracheary element-inducing factor was partially purified. The partially purified substance was a high molecular weight-arabinogalactan protein and designated "xylogen".(3) We found that brassinosteroids were synthesized prior to Stage 3 of differentiation and their biosynthesis was regulated by a coordinated increase in transcription of genes encoding brassinosteroid biosynthesis. In addition, our finding that castasterone, an active brassinosteroid, is secreted preferentially out of the cell and accumulates in medium, suggests that brassinosteroids function at extracellular surface of the cell.(4) Using a phage display subtraction method, we succeeded in isolating of three novel antibodies recognizing cell walls of specific vascular cells. Less

1。拟南芥血管突变体的分析，VAN1-VAN7：拟南芥血管突变体的分子分析，带有PathB8-GUS或PTED3-GUS融合基因的VAN1-VAN7 vAN1-VAN7揭示了这些突变体的缺陷，是由异常模式形成引起的。此外，我们表明，血管网络的连续性对遗传病变的敏感性比其整体结构更敏感，从而支持扩散反应的预生产模型2。胡萝卜体细胞胚发生过程中HD-ZIP同源基因的分析。我们分析了6个HD-ZIP同源基因CHB1-CHB6的表达模式，并在胡萝卜体细胞胚胎发生过程中具有原位杂交，并揭示了所有基因在胚胎的后期与血管细胞相关表达，即CHB6在Procambium和其他不同的血管细胞中特异性表达。这些结果表明，HD-ZIP同源基因基因与血管形成有关3。对血管的分析…与体外Zinnia系统的更多R细胞分化，其中叶叶叶叶细胞分化为血管细胞。（1）我们揭示了体外Zinnia培养系统中存在不同种类的血管细胞。（2）我们建立了新的培养方法，通过这种气管元素诱导的因子是部分含有纯化的。部分纯化的物质是一种高分子量 - 阿拉伯分泌蛋白，并指定为“木基生成”。（3）我们发现，在分化的第3阶段之前，在基因的转录增加了编码铜氨基固醇生物合理的基因的转录中，在分化的第3阶段之前合成了铜氨基固醇。此外，我们的发现，即castasterone是一种活性的胸腺固醇，更优选地分泌出细胞，并在培养基中积聚，这表明铜氨基固醇在细胞的细胞外表面上起作用。（4）使用噬菌体显示减法方法，我们成功地隔离了三种新型抗体识别特定血管细胞的细胞壁的新型抗体。较少的