Molecular mechanism of the acquirement and restriction of differentiation potency in plant cells

植物细胞分化能力获得和限制的分子机制

基本信息

批准号：
07454215
负责人：
FUKUDA Hiroo
金额：
$ 5.06万
依托单位：
University of Tokyo (1996)Tohoku University (1995)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454215/
关键词：
DNase Antisense DNA Gene transfer Brassinosteroid Zinnia elegans Tracheary element Cell death Transdifferentiation nuclease 植物ホルモン脱分化

项目摘要

1.Analysis of the acquirement of pluripotency of transdifferentiation of isolated mesophyll cellsWe isolated 12 cDNA clones whose transcripts are expressed preferentially in isolated Zinnia mesophyllcells. These clones were divided into teree groups, 1)wound or infection-induced genes, 2)genes encoding proteins involved in protein synthesis, and 3) others. mRNAs for all of these clones were induced by wounding within 12 h of culture but thereafter chenged in hormone-dependent manners, suggesting that the early process involves the activation of protein synthesis.2.Analysis of the restriction of the potency of differentiationa) Analysis of tracheary element precursor cell-specific expression of TED3 promoterThe analysis of transgenic Arabidopsis with chimeric genes of TED3 promoter and GUS gene indicated that this promoter directed tracheary element precursor cell-specific expression.b) Analysis of TED3 functionWe introduced an antisense DNA of TED3 gene into Zinnia leaves and induced many transformed hairy roots. Among the root clones, clones in which the accumulation of TED3 mRNAs were suppressed showed the inhibition of root growth. Taking together with the expression of TED3 mRNAs is localized in tracheary element precursor cells, we presented a hypothesis that TED3 product may be responsible for the elongation of tracheary elements.c)Analysis of cell death processWe isolated cDNA clones for cysteine protease and DNase which may be involved in autolytic process in differentiating tracheary elements. The mRNAs for these clones expressed in very similar pattern, transiently just before vacuole disruption. This suggests a common mechanism of gene expression in relation to cell death process.Based on these results, we presented a hypothesis on the Change in the potency of differentiation.

1.分离的叶肉细胞分离的12个cDNA克隆的跨分化的多能性的获得的分析，其转录物在叶酸的分离zinnia lesophyllcells中优先表达。这些克隆分为TEREE组，1）伤口或感染诱导的基因，2）编码涉及蛋白质合成的蛋白质的基因，以及3）其他基因。所有这些克隆的mRNA是通过在培养的12小时内诱导的，但此后以激素依赖性的方式进行了诱导，这表明早期过程涉及蛋白质合成的激活。2。分化效力的限制的分析）分析的分析）分析了ted3促进者的the the the the the the the the the the the the the the the the the the chim theperiant theperies theperiant thepersiS gimipis chimICISIS的构成型的分析。基因表明该启动子将气管元件前体细胞特异性表达。b）TED3函数分析我们将TED3基因的反义DNA引入了Zinnia叶片中，并引起了许多转化的毛根。在根克隆中，抑制TED3 mRNA积累的克隆显示出根生长的抑制作用。与TED3 mRNA的表达一起位于气管元素前体细胞中，我们提出了一个假设，即TED3乘积可能是导管气管元素的伸长。这些克隆的mRNA以非常相似的模式表达，直到液泡破坏之前。这表明基因表达与细胞死亡过程有关的常见机制。基于这些结果，我们提出了关于分化效力的变化的假设。