Effects of Mg-load on the contraction of uterus in sponteneously diabetic mice

镁负荷对自发性糖尿病小鼠子宫收缩的影响

基本信息

批准号：
08680050
负责人：
AOMINE Masahiro
金额：
$ 1.6万
依托单位：
Nakamura Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680050/
关键词：
Mg-load Diabetes mellitus Mouse Uterus Contraction Oxytocin

项目摘要

First, we investigated effects of external magnesium (Mg) on the contractile tension of uterus under the condition of Mg-unload, using spontaneously diabetic mice (KK/tA9 and the non-diabetic mice (c57/BL). Both KK/Ta and C57/BL mice were divided into the folloowing three groups : a group fed with normal diet (N-group) ; a group fed with normal diet, accompanied by pohysical exercice (whirl cage running)(T-group) ; a group fed with restricted diet (a half of normal volume), accompanied by the physical exercice (TD-group). External Mg decreased the amplitude and frequency of the contractile tension of uttlerus in a concentration dependent manner. IC_<50> of Mg in the amplitude and frequency of the tension in both mice was the smallest in N-group, followed by T-group and TD-group in the order. This tendency was more eminent in diabetic mice. Secondly, the inhibitive effects of external Mg on the contractile tension of utterus were studied, using Mg-load mice, and compared with Mg-unload mice. Mg-load was done by 1. p. injection of 2M MgCl_2 for 2 to 3 weeks. In Mg-load KK/Ta mice, serum Mg concentration was significantly higher than in Mg-load C57/Bl, whereas uterus Mg concentration was almost same in both mice. In Mg-unload mice, C57/BL uterus was more sensitive to external Mg than KK/Ta uterus. In Mg-load mice, however, the difference between KK/Ta and C57/BL disappeared, suggesting the relation between the contractility and the Mg concentration in the tissue. In oxytocin-induced contracture, Mg-load suppressed the amplitude of the contracture and significantly shortened the time to the peak contracture. These resyults strogly suggested that Mg may affect the affinity between oxytocin and the receptors, and the density of the receptors.

首先，我们使用自发的糖尿病小鼠（KK/TA9和非糖尿病小鼠（C57/BL）（KK/TA和C57/BL小鼠均由三组饮食培养（N-Grolto），我们使用自发的饮食（KK/TA9和非糖尿病小鼠），研究了由饮食养成的饮食（N-diet（N-diet），n grof controve（kk/ta9和非糖尿病小鼠），我们研究了由三组养育的饮食（N-diet）（N-north normal for norker）（N-diet（N）（n-diet）（N-diet（N），我们均用饮食养成了n-grof（n-diet）（n-nork fornal：n grouct fornal：N-肥胖的锻炼（旋转笼子）（t组）；伴随着饮食限制的一半，伴随着体育锻炼（TD组）。在糖尿病小鼠中，该趋势在阶段更为明显。 MG负载通过1。P进行。注射2M MGCL_2 2至3周。在MG载量KK/TA小鼠中，血清MG浓度显着高于Mg载荷C57/BL，而两只小鼠的子宫毫克浓度几乎相同。在MG-UNLOAD小鼠中，C57/BL子宫比KK/TA子宫更敏感。然而，在MG负载小鼠中，KK/TA和C57/BL之间的差异消失了，这表明收缩力与组织中的MG浓度之间的关系。在催产素诱导的染色体中，MG负载抑制了缔约合的幅度，并大大缩短了峰值缔约峰的时间。这些重生表明，MG可能会影响催产素和受体之间的亲和力，以及受体的密度。