Does nutrients modulate food-intake behavior? Measurement of neurotransmitters using in vivo brain microdialysis in diabetic rat.

营养素是否会调节食物摄入行为？

基本信息

批准号：
10680157
负责人：
AOMINE Masahiro
金额：
$ 2.24万
依托单位：
Nakamura Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In the present work, the following studies were undertaken : (Exp.1) Whether the abnormal behavior of food intake in diabetic animals just like human diabetic patients exists or not ; (Exp.2) Whether the abnormality of food-intake behavior will be responsible for the altered release of neurotransmitters in the diabetic brain or not, presumably permitting the frailty to stressors ; (Exp.3) Whether among many foods coffee and its main components are effective in stress-ralaxation or not. (Exp.1) WBN/Kob rat and KK/Ta mouse as spontaneously diabetic animal, and normal Wistar-rats and C57/BL mouse as thc control were used. In diabetic animals the spontaneous locomotor activity decreased. In both normal and diabetic animals, acute stress (100 min-restraint) elicited the decrease in food- and water-intake, and the circadian rhythm of food- and water-intake was disturbed especially in diabetic mice. (Exp.2) In this experiment the hippocampal levels of serotonin (5-HT) and dopamine (DA) of WBN … More /Kob rat, streptozotocin (STZ)-induced diabetic rat and normal Wistar rat were measured using in vivo brain microdialysis method. The 5-HT level in normal Wistar rat was the largest value, followed by that in STZ-rat and WBN/Kob rat in that order. The 5-HT level in WBN/Kob rat was about a half of that of the normal rat, and the DA level in WBN/Kob rat was about one-tenth of that of the normal rat. The experiment using several ion channel blockers suggested the dysfunction of ion channels in diabetic synapse. (Exp.3) Acute restraint stress led to prominent increase in the hippocampal levels of 5-HT.In normal rat, "peak 5-HT value during 2nd restraint/peak 5-HT value during 1st restraint" (stress-ralaxing index) in pretreatment of coffee, caffeine, chlorogenic acid and saline was about 37%, 34%, 130% and 85%, respectively. This suggests that the administration of coffee contributes to relaxation of restraint stress and further this effect of coffee could be due to the caffeine which is contained in the coffee. On the other hand, in WBN rat, the stress-ralaxing index in pretreatment of coffee, caffeine, and saline was about 45%, 51%, 68% and 85%, respectively, suggesting the frailty to stressors in diabetic animals. Less

本工作进行了以下研究：（实验1）糖尿病动物是否存在与人类糖尿病患者一样的摄食行为异常；（实验2）是否会出现摄食行为异常；是否与糖尿病大脑中神经递质释放的改变有关，可能导致对压力源的脆弱；（实验3）在许多食物中，咖啡及其主要成分是否能有效缓解压力。 (实验1)使用WBN/Kob大鼠和KK/Ta小鼠作为自发性糖尿病动物，并使用正常Wistar大鼠和C57/BL小鼠作为对照。在糖尿病动物中，正常和糖尿病动物的自发运动活性均下降。，急性压力（100分钟限制）引起食物和水摄入量减少，食物和水摄入量的昼夜节律受到干扰，尤其是在糖尿病患者中(实验 2) 在本实验中，使用体内方法测量了 WBN … More /Kob 大鼠、链脲佐菌素 (STZ) 诱导的糖尿病大鼠和正常 Wistar 大鼠的海马血清素 (5-HT) 和多巴胺 (DA) 水平。脑微透析法中5-HT水平以正常Wistar大鼠最高，其次为STZ大鼠、WBN/Kob大鼠。 WBN/Kob 大鼠的 DA 水平约为正常大鼠的一半，WBN/Kob 大鼠的 DA 水平约为正常大鼠的十分之一。使用几种离子通道阻滞剂的实验表明离子通道功能障碍。 (实验 3) 急性束缚应激导致海马 5-HT 水平显着升高。在正常大鼠中，“第二次束缚期间的峰值 5-HT 值/峰值 5-HT”咖啡、咖啡因、绿原酸和盐水预处理中的“第一次约束值”（压力放松指数）分别约为37%、34%、130%和85%。这表明咖啡的服用有助于放松约束另一方面，在 WBN 大鼠中，咖啡、咖啡因和咖啡因预处理中的压力松弛指数。盐水分别约为 45%、51%、68% 和 85%，表明糖尿病动物对压力源的脆弱性较小。