Does nutrients modulate food-intake behavior? Measurement of neurotransmitters using in vivo brain microdialysis in diabetic rat.

营养素是否会调节食物摄入行为？

基本信息

批准号：
10680157
负责人：
AOMINE Masahiro
金额：
$ 2.24万
依托单位：
Nakamura Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In the present work, the following studies were undertaken : (Exp.1) Whether the abnormal behavior of food intake in diabetic animals just like human diabetic patients exists or not ; (Exp.2) Whether the abnormality of food-intake behavior will be responsible for the altered release of neurotransmitters in the diabetic brain or not, presumably permitting the frailty to stressors ; (Exp.3) Whether among many foods coffee and its main components are effective in stress-ralaxation or not. (Exp.1) WBN/Kob rat and KK/Ta mouse as spontaneously diabetic animal, and normal Wistar-rats and C57/BL mouse as thc control were used. In diabetic animals the spontaneous locomotor activity decreased. In both normal and diabetic animals, acute stress (100 min-restraint) elicited the decrease in food- and water-intake, and the circadian rhythm of food- and water-intake was disturbed especially in diabetic mice. (Exp.2) In this experiment the hippocampal levels of serotonin (5-HT) and dopamine (DA) of WBN … More /Kob rat, streptozotocin (STZ)-induced diabetic rat and normal Wistar rat were measured using in vivo brain microdialysis method. The 5-HT level in normal Wistar rat was the largest value, followed by that in STZ-rat and WBN/Kob rat in that order. The 5-HT level in WBN/Kob rat was about a half of that of the normal rat, and the DA level in WBN/Kob rat was about one-tenth of that of the normal rat. The experiment using several ion channel blockers suggested the dysfunction of ion channels in diabetic synapse. (Exp.3) Acute restraint stress led to prominent increase in the hippocampal levels of 5-HT.In normal rat, "peak 5-HT value during 2nd restraint/peak 5-HT value during 1st restraint" (stress-ralaxing index) in pretreatment of coffee, caffeine, chlorogenic acid and saline was about 37%, 34%, 130% and 85%, respectively. This suggests that the administration of coffee contributes to relaxation of restraint stress and further this effect of coffee could be due to the caffeine which is contained in the coffee. On the other hand, in WBN rat, the stress-ralaxing index in pretreatment of coffee, caffeine, and saline was about 45%, 51%, 68% and 85%, respectively, suggesting the frailty to stressors in diabetic animals. Less

在目前的工作中，进行了以下研究：（Exp.1）糖尿病动物中食物摄入的异常行为是否像人类糖尿病患者一样；（exp.2）食物智能行为的绝对性是否会导致糖尿病大脑中神经递质的释放改变，这大概允许脆弱的压力源；（实验3）在许多食品咖啡及其主要成分中是否有效地折应力。（exp.1）使用WBN/KOB大鼠和KK/TA小鼠作为赞助的糖尿病动物，以及正常的Wistar-鼠和C57/BL小鼠作为THC对照。在正常动物和糖尿病动物中，急性应激（100分钟的折射）引起了食物和水的减少，而在糖尿病小鼠中尤其干扰了食物和水的昼夜节律。（exp.2）在该实验中，使用体内脑微透析法中测量了使用WBN…更多 /KOB大鼠，链球菌链球菌素（STZ）诱导的糖尿病大鼠和正常Wistar大鼠的5-羟色胺（5-HT）和多巴胺（DA）的海马水平。正常Wistar大鼠中的5-HT水平是最大的值，其次是STZ-RAT和WBN/KOB大鼠的值。 WBN/KOB大鼠的5-HT水平约为正常大鼠的一半，WBN/KOB大鼠的DA水平约为正常大鼠的十分之一。使用几个离子通道阻滞剂的实验表明糖尿病突触中离子通道的功能障碍。（exp.3）急性约束应力导致5-ht.in正常大鼠海马水平的显着增加，“在第1个约束/峰值5-ht值期间的峰值5-HT值”（咖啡因，氯化酸和盐水约为37％，34％，34％，134％，130％，134％，130％和85％），在第1个约束期间的5-HT值/峰值5-HT值”（应力 - 偏向指数）。这表明咖啡的给药有助于放松约束压力，进一步的咖啡影响可能是由于咖啡因所含的咖啡因所致。另一方面，在WBN大鼠中，咖啡，咖啡因和盐水预处理中的应力 - 中央轴指数分别约为45％，51％，68％和85％，这表明糖尿病动物的压力源脆弱。较少的