Prevention of hypoxic brain damage by in vivo transfection through the carotid artery in cirsulatory arrest

通过颈动脉体内转染预防循环骤停中的缺氧性脑损伤

• 批准号: 08671526
• 负责人: FUKUSHIMA Norihide
• 金额: $ 1.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671526/
• 关键词: gene transfection; bcl-2; HSP70; HVJ-liposome; hypothermic circulatory arrest; tolerance in ischemia-reperfusion injury; HSP70; 脳虚血

Efficient gene transfer method into the whole heart through the coronary artery with Hemagglutinating Virus of Japan liposoine this method represents a novel in vivo gene transfar technique for the heart and thus may provide a new tool for this project and research.In vivo gene transfection with heat shock protein 70 and bcl-2 enhance myocardial and liver cell tolerance to ischemia-reperfusion injury : overexpressed HSP 70 and Bcl-2 in rat hearts and livers plays a protective role in ischemia- reperfusion injury, suggesting the possibility that gene transfection with HSP 70 and bcl-2 may become a novel method for the organ protection.Gene transfar method into the brain through the carotid artery in hypothermic circulatory arrest HVJ-liposome was used for gene transfection of oligonucleotide labeled with fluorescein into the rat brain through the carotid artery. Fluorescein isothiocyanate was detected in the nuclei.In vivo gene transfection with heat shock protein 70 and bcl-2 into the brain for the protection in ischcmia-reperfusion injury : HSJ 70 and bcl-2 are transfered into the rat brain.

使用日本脂质体血凝病毒通过冠状动脉将基因有效转移到整个心脏的方法代表了一种新颖的心脏体内基因转移技术，因此可能为该项目和研究提供新的工具。热体内基因转染休克蛋白70和bcl-2增强心肌和肝细胞对缺血再灌注损伤的耐受性：大鼠心脏和肝脏中过表达的HSP 70和Bcl-2起到保护作用缺血再灌注损伤，提示HSP 70和bcl-2基因转染可能成为一种新的器官保护方法。低温停循环中颈动脉基因转入脑内采用HVJ-脂质体进行基因转染荧光素标记的寡核苷酸通过颈动脉进入大鼠大脑。细胞核中检测到异硫氰酸荧光素。 将热休克蛋白70和bcl-2体内基因转染到脑中以保护缺血再灌注损伤：将HSJ 70和bcl-2转入大鼠脑中。

项目成果

Suzuki K et al.: "In vivo gene transfection with Heat Shock Protein 70 enhances myocardial tolerance to ischemia-reperfusion injury in rat." J.Clin.Invest.99(7). 1645-1650 (1997)

Suzuki K 等人：“用热休克蛋白 70 进行体内基因转染可增强大鼠心肌对缺血再灌注损伤的耐受性。”

Yamabe K,Sawa Y,Matsuda H,et al.: "Prevention of hypoxic liver cell necrosis by in vivo human bcl-2 gene transfection." Biochem.Biophy.Res.Commun.243. 217-223 (1998)

Yamabe K，Sawa Y，Matsuda H，et al.：“体内人bcl-2基因转染预防缺氧性肝细胞坏死”。

Yamabe K.et al.: "Prevention of hypoxic liver injury by in vivo transfection of the human bcl-2 gene." Transplant.Proceed.29. 384-385 (1997)

Yamabe K.等人：“通过体内转染人 bcl-2 基因预防缺氧性肝损伤。”

Sawa Y. et al: "Efficient gene transfer method into the whole heart through the coronary artery with Hemaggulutinating Virus of Japan liposome." J.Thorac.Cardiovasc.Surg.113(3). 512-519 (1997)

Sawa Y.等人：“使用日本血凝病毒脂质体通过冠状动脉将基因有效转移到整个心脏。”

Yamabe K,Sawa Y.Matsuda H,et al.: "Prevention of hypoxic liver injury by in vivo transfection of the human bcl-2 gene." Transplant. Proceed.29. 384-385 (1997)

Yamabe K、Sawa Y.Matsuda H 等人：“通过体内转染人 bcl-2 基因预防缺氧性肝损伤”。