The prognosic value of the transcription of tumor growth-related genes in endoscopic biopsy specimens from patients with malignancies of digestive tracts

消化道恶性肿瘤内镜活检标本中肿瘤生长相关基因转录的预后价值

• 批准号: 08671460
• 负责人: KATANO Mitsuo
• 金额: $ 1.41万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671460/
• 关键词: biopsy specimens; gastric carcinoma; mRNA; molecular and biological analysis; RT-PCR; survival time; prognostic factor; multivariate analysis

The tumor biopsy specimens were obtained during preoperative endoscopy of patients with gastric carcinoma. The total RNA from each biopsy specimen was isolated and a reverse trascriptase-polymerase chain reaction analysis (RT-PCR) was used to analyze the transcription of tumor growth-related genes. The factors examined included cyclin D1 and cyclin E as cell cycle-related factors, urokinase-type plasminogen activator and 72-kd type IV collagenase as invasion-related ones, vascular endothelial growth factor and platelet-derived growth factor-A as angiogenesis-related ones, and transforming growth factor b and interleukin-10 as immunosuppression-related ones.Results :(1) The incidence of PDGF-A and TGF-b mRNA expression was preoperative indicator of lymph node involvement in early and advanced gastric cartcinomas, respctively.(2) PDGF-A and TGF-b were preoperative prognostic indicators.(3) Preoperative evaluation of malignant potential of each carcinoma may lead to a more reasonable classification for gastric carcinomas.

肿瘤活检标本是在胃癌患者的术前内窥镜检查过程中获得的。从每个活检标本中分离总RNA，并使用逆转录酶聚合酶链反应分析（RT-PCR）来分析肿瘤生长相关基因的转录。检测的因子包括细胞周期相关因子cyclin D1和cyclin E，侵袭相关因子尿激酶型纤溶酶原激活剂和72-kd IV型胶原酶，血管生成相关因子包括血管内皮生长因子和血小板源性生长因子-A。结果：(1)PDGF-A、TGF-b的发生率mRNA表达量分别是早期和晚期胃癌术前淋巴结受累的指标。(2)PDGF-A和TGF-b是术前预后指标。(3)术前评估每种癌的恶性潜能可能会导致更合理的预后判断。胃癌的分类。

项目成果

Anan K,Morisaki T,Katano M,Ikubo A,Tsukahara Y,Uchiyama A,Kuroki S,Tanaka M,Torisu M: "Preoperative assessment of tumor angiogenesis by vascular endothelial growth factor mRNA expression in homogenate sample of breast carcinoma : fine needle aspirates Vs.

Anan K、Morisaki T、Katano M、Ikubo A、Tsukahara Y、Uchiyama A、Kuroki S、Tanaka M、Torisu M：“通过乳腺癌匀浆样本中血管内皮生长因子 mRNA 表达对肿瘤血管生成进行术前评估：细针抽吸

Nakamura M: "A new prognostic strategy for gastric carcinoma:mRNA expression of tunsor growth-related factors in endoscopic biopsy specimens" Ann Surg. 226. 35-42 (1997)

Nakamura M：“胃癌的新预后策略：内窥镜活检标本中肿瘤生长相关因子的 mRNA 表达”Ann Surg。

片野光男: "癌性腹膜炎治療を通して学ぶ臨床腫瘍免疫学" 癌と化学療法社, 230 (1997)

Mitsuo Katano：“通过治疗癌性腹膜炎学到的临床肿瘤免疫学”癌症和化疗出版，230（1997）

Katano M: "Prognostic value of platelet-derived growth factor-A (PDGF-A) in gastric carcinoma" Ann Surg. (in press).

Katano M：“血小板衍生生长因子-A (PDGF-A) 在胃癌中的预后价值”Ann Surg。

Katano M: "Melanoma antigen-encoding gene-1 expression in invasive gastric carcinoma:correlation with stage of disease" J Surg Oncol. 64. 195-201 (1997)

Katano M：“浸润性胃癌中黑色素瘤抗原编码基因 1 的表达：与疾病阶段的相关性”J Surg Oncol。