Radio- or chemo-sensitivity and analysis of gene transduction for induction of apoptosis for human tumor cell

放射或化学敏感性以及诱导人肿瘤细胞凋亡的基因转导分析

• 批准号: 08671007
• 负责人: SAITO Yoshihiro
• 金额: $ 1.34万
• 依托单位: Gunma University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671007/
• 关键词: radiosensitivity; chemosensitivity; apoptosis; paclitaxl; combination therapy of radiation with chemotherapy; supra-additive effect; Apoptosis; Radiosensitivity; Chemosensitivity; Paclitaxol; Taxol

Apoptotic death is reported to be important factor for radio- or chemo-sensitivity to tumor.Purpose is to investigate the differences between two rat yolk sac tumour cell lines with different radiosensitivities by different induction of apoptotic cells in paclitaxl sensitivity and in the sensitizing effects of paclitaxl in combination with irradiation. NMT-1 is a parent radiosensitive cell line and NMT-1R is a variant radioresistant cell line. Clonogenic assay demonstrated almost the same paclitaxl sensitivity with Do of 5.15 nM in NMT-1 and 5.02 nM in NMT-1R.Many apoptotic cells and DNA ladder formations were observed at 24 h after exposure to paclitaxl in both cell lines. The incidences of DNA fragmentation after 24 h exposure to 20 nM paclitaxl were 12.4 (]SY.+-。[) 3.3 % for NMT-1, and 13.0(]SY.+-。[) 1.9 % for NMT-1R.Paclitaxl showed a supra-additive effect in combination with radiation in both cell lines at 12 h after paclitaxl treatment only when the accumulation of cells in the G2M phase reached its peak.Paclitaxl had the same degree of cytotoxic effect in two cell lines with different radiosensitivity due to the induction of apoptosis. A supra-additive effect to radiation was observed with 12 h pretreatment in both cell lines. Paclitaxl may be effective for tumors with a component of different radiosensitivity in combination with irradiation.

据报道，凋亡死亡是对肿瘤的射线或化学敏感性的重要因素。Purpose是为了在卵黄囊肿瘤细胞系的toro差异，其在与受照射的结合中的Tet Izing Ising效应中不同的凋亡敏感性不同。在NMT-1中，NMT-1R中的DO为5.15 nm几乎具有saclitaxl敏感性。在24小时，NMT-1R中的NMT-1 R. MARY凋亡细胞和DNA梯子形成。 - 。由于电感凋亡，在两个细胞透视上，您的G2M相在两个细胞线中达到了相同程度的细胞毒性。结合辐照。

项目成果

Saito Yoshihiro: "Cytotoxic effect of paclitaxl (taxol) either alone or in combination with irradiation in two rat yolk sac tumour cell lines with different radiosensitivities in vitro" International Journal of Radiation Biology. 73・2. 225-231 (1998)

Saito Yoshihiro：“紫杉醇（紫杉醇）单独或与体外放射敏感性不同的两种大鼠卵黄囊肿瘤细胞系的细胞毒性作用”国际放射生物学杂志73·2（1998）。

Saito Yoshihiro: "Cytotoxic effect of paclitaxl (taxol) or in combination with radiation in two rat yolk sac tumor cell lines with different radiosensitivities" Jpn.J.Cancer Clin.44-2. 243-244 (1998)

Saito Yoshihiro：“紫杉醇（紫杉醇）或与辐射组合对两种具有不同放射敏感性的大鼠卵黄囊肿瘤细胞系的细胞毒性作用”Jpn.J.Cancer Clin.44-2。

斉藤 吉弘: "放射線感受性の異なる腫瘍細胞株に対するpaclitaxel(Taxol)と放射線との併用効果" 癌の臨床. 44・2. 243-244 (1998)

Yoshihiro Saito：“联合使用紫杉醇（紫杉醇）和放射线对不同放射敏感性肿瘤细胞系的影响”癌症诊所 44・2（1998）。