The roles of growth factors and cytokine in retinal neovascularization.

生长因子和细胞因子在视网膜新生血管形成中的作用。

• 批准号: 07557263
• 负责人: SAITO Yoshihiro
• 金额: $ 1.86万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557263/
• 关键词: neovascularization; Agerelated macular degeneration; thalidomide; laser; retina; bFGF; Diabetes; Retinopathy of prematurity; 加齢黄斑変性; 脈絡膜; 光化学療法; フルオレスチンナトリウム

We developed new animal models of ocular neovascularization. A rat model of subretinal neovascularization was made by a b-FGF containing pellet implanted between sensory retinal and retinal pigment epithelium. The subretinal neovascularization was very similar to human age-related macular degeneration histologically and histopathologically. Another model was preretinal neovascularization in rats, which was made by artificial retinal vein occlusion with photodynamic (Ar laser and fluorescein-Na) thrombosis in rat. This was the first rat model of preretinal neovascularization.Thalidomide is a well known teratogen in human. The drug which had an inhibitory effect in angiogenesis for rabbit corneal neovascularization is the only antiangiogenesis agent that is currently ready for human efficacy trials. We investigated the inhibitory effect of thalidomide using preretinal neovascularization model. Though there was no significant difference, it tended to have an inhibitory effect on retinal neovascularization in rats.We investigated the roles of growth factors and cytokines in pre- and sub- retinal neovascularization using rat models. We found a-FGF,b-FGF and VEGF had significant role in the development of above neovascularizations.We also attempted to improve the surgical result or clinical knowledge of diseases which develop the preretinal and/or subretinal neovascularization : retinopathy of prematurity, diabetes, age-related macular degeneration.

我们开发了眼科新动物的新动物模型。视网膜下新血管形成的大鼠模型是由含有感官视网膜和视网膜色素上皮之间植入的颗粒的B-FGF进行的。视网膜下新血管形成与人年龄相关的黄斑变性在组织学和组织病理学上非常相似。另一个模型是大鼠的前神经性新血管形成，这是由人工视网膜静脉闭塞与大鼠光动力学（AR激光和荧光素-NA）血栓形成的。这是前新的新生血管化的大鼠模型。地下层是人类的众所周知的致病原模型。对兔角膜新血管形成的血管生成具有抑制作用的药物是目前为人类效力试验做好准备的唯一抗血管生成剂。我们使用前新生血管化模型研究了沙利度胺的抑制作用。尽管没有显着差异，但它倾向于对大鼠的视网膜新血管形成产生抑制作用。我们研究了使用大鼠模型研究生长因子和细胞因子在前和视网膜下新血管形成中的作用。我们发现A-FGF，B-FGF和VEGF在上述新血管造成的发展中起着重要作用。我们还试图改善发展前和/或下视网膜下新生血管的外科手术结果或临床知识：早产，糖尿病，糖尿病，糖尿病，，糖尿病，，糖尿病，，糖尿病性视网膜病变。与年龄相关的黄斑变性。

项目成果

Tsujikaw M.Saito et al.: "Secondary vitrecfony for the treatment of macular〜" Ophthalmic Surgery and lasers. (印刷中).

Tsujikaw M.Saito 等人：“治疗黄斑的继发性玻璃体切除术 ~”眼科手术和激光（出版中）。

Sawa M,Kamei M,Tsujikawa M,Ohji M,Saito, Y,et al.: "Outer blood-retinal barrior reconstruction after surgical removal of choroidal neovascularization." ARVO (May 11 16,1997).

Sawa M，Kamei M，Tsujikawa M，Ohji M，Saito，Y，et al.：“手术切除脉络膜新生血管后的外血视网膜屏障重建。”

Saito, Y,et al.: "A lens change and related symptoms during rapid tightening of metabolic control in diabetes." ARVO (May 11-16,1997).

Saito, Y 等人：“快速加强糖尿病代谢控制过程中的晶状体变化和相关症状。”

Saito Y.et al: "Lens Changes at rapid tightening of metaholic" Cancet. 347. 1764 (1996)

Saito Y.et al：“镜片在元醇快速收紧时发生变化”Cancet。

Nakabayashi M,Fujikado T,Tsujikawa M,Ohji M,Saito Y,et al.: "Fixation patterns of macular hole after successful vitreous surgery." ARVO (May 11-16,1997).

Nakabayashi M，Fujikado T，Tsujikawa M，Ohji M，Saito Y，et al.：“成功玻璃体手术后黄斑裂孔的固定模式。”