Molecular biological changes of ADP/ATP carrier protein in cardiomyopathic hamsters

心肌病仓鼠ADP/ATP载体蛋白的分子生物学变化

• 批准号: 08670827
• 负责人: HAYASHI Yuusaku
• 金额: $ 0.7万
• 依托单位: The Jikei University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670827/
• 关键词: ATP; ADP carrier protein; mitochondrial membrane; cardiomyopathy; hypertrophy; diabetic hearts; adaptation; 肥大心; ノーザンブロット; 拡張形心筋症; ミトコンドリア遺伝子; 圧負荷肥大心; ミトコンドリア

The ATP/ADP carrier protein (AAC) is an integral protein present in the inner mitochondrial membrane that performs the exchange of cytoplasmic and intramitochondrial ADP and ATP.The ACC content of the myocardium was studied in J-2-N cardiomyopathic hamster, renovascular hypertensive rats and diabetic rats.The content of ACC protein was significantly decreased in cardiomyopathic hamster. On the other hand, ACC m-RNA level was increased in cardiomyopathic hamster. In renovascular hypertensive rat (2K-1C), left ventricular hypertrophy and abnormal changes of myosin isoenzyme pattern were observed 8 weeks after an operation. The content of ACC protein was significantly decreased in operated rats. In streptozotocin-induced diabetic rats, the content of ACC protein was significantly increased.These results suggest that the changes of the AAC protein play an important role in the pathogenesis of hemodynamic alteration of cardiomyopathy, hypertrophy and diabetic hearts. Moreover, these changes were considered to be an adaptation which contribute to maintain efficient force development with low oxygen and energy utilization in several cardiac deceases.

ATP/ADP载体蛋白（AAC）是内部线粒体膜中存在的积分蛋白大鼠和糖尿病大鼠。心肌病仓鼠中ACC蛋白的含量显着降低。另一方面，心肌病仓鼠中ACC M-RNA水平升高。在肾血管高血压大鼠（2K-1C）中，左心室肥大和肌球蛋白同工酶模式的异常变化在手术后8周观察到。在手术大鼠中，ACC蛋白的含量显着降低。在链蛋白酶诱导的糖尿病大鼠中，ACC蛋白的含量显着增加。这些结果表明，AAC蛋白的变化在心肌病，肥大和糖尿病心脏的血液动力学改变的发病机理中起重要作用。此外，这些变化被认为是一种适应性，有助于在几种心脏脱离中保持氧气和能量利用率低的有效力发展。

项目成果

加藤光敏他: "続心臓代謝実験法 「ADP/ATP carrier protein測定法」" 株式会社六法出版, 541-544 (1998)

加藤光俊等：“继续心脏代谢实验方法“ADP/ATP载体蛋白测定方法”，六方出版株式会社，541-544(1998)

Li-Mei Liu et al.: "The alterrations of myosin isoenzymes and ADP/ATP carrier (AAC) in Goldblatt hypertensive rats" J.Mol.Cell.Cardiol.28. A336 (1996)

Li-Mei Liu 等人：“Goldblatt 高血压大鼠中肌球蛋白同工酶和 ADP/ATP 载体 (AAC) 的改变”J.Mol.Cell.Cardiol.28。