Studies on the functional substances from young fruits of Satsumamandarin and Japanese pear.

萨摩柑橘和梨幼果功能物质的研究。

• 批准号: 08660037
• 负责人: FUJITA Shuji
• 金额: $ 1.41万
• 依托单位: Saga university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660037/
• 关键词: Citrus fruit; Ascorbic acid; Ascorbic acid analysis; Antioxidative substance; Polyphenol; Japanese pear; Biofunctional substance; Polyphenol oxidase; ニホンナシ; 抗酸化能; ポリフェノール類

Twenty cultivars of citrus fruits were harvested two stages of development : young fruit (weight about 2.5 gper fruit) and fully ripe fruit of which the ascorbic acid oxidase (AAO) activities were determined. Among the different cultivars, the specific activities of AAO in the young fruit ranged from 47 to 2120 units per mg protein, while fully ripe fruits had AAO ranging from 18 to 98 unit per mg protein. Significant differences were also found between tissue types in the AAO activity with the higest activity in albedo (64-70%), followed by the flavedo (26-34%) and the pulp (2-3%).AAO obtained from the young fruits of Satsuma mandarin was utilized for the enzymatic determination of ascorbic acid (AsA) based on the difference spectral method as a possible new source of AAO,and the results was compared to cucumber AAO.A linear relationship was observed between AAO activity calculated from the maximum peak height of the difference spectra and the AsA concentration in the ranges of 0-100 ug/ml. The values of AsA determined by the enzyme were also comparable to the valuse obtained using cucumber AAO.Thus, AAO from the young fruit could provide a cheaper source of the enzyme for AsA determination.The crude extract obtained from young fruits of Japanese pear had strong antioxidative activity for linoleic acid oxidation as well as BHA.The extract contained polyphinols such as chlorogenic acid and catechin. These polypenols also indicated antioxidative activity for Jinoleic acid oxidation. The young fruit extract showed antioxidative activity for about two month. These results invesitgated that the extract from young fruits of Japanese pear had been employed for a natural aitioxidant for fatty foods.

收集了二十种柑橘类水果品种的发展两个阶段：年轻的水果（约2.5 gper果实）和完全成熟的果实，确定了抗坏血酸氧化酶（AAO）活性。在不同的品种中，AAO在年轻果实中的特定活性范围为每毫克蛋白质蛋白47至2120单位，而完全成熟的水果的AAO范围为每毫克蛋白的18至98个单位。在AAO活性中，组织类型之间的组织类型在反照率（64-70％）中的组织类型之间也存在显着差异，其次是Flavedo（26-34％）和果肉（2-3％）（2-3％）（2-3％）。从Satsuma Mandarin的年轻果实中获得的AAO用于新的方法，用于邻苯二甲酸酯（ASA）的差异（ASA）的差异（ASA）的差异（ASA）的差异（ASA）的差异（ASA）的差异（ASA）的差异（ASA）的差异（ASA）属于ASACIR酸（ASA）的差异（satsarin）。 AAO，并将结果与​​Cucumber AAO进行了比较。从差异光谱的最大峰高度计算出的AAO活性与0-100 ug/mL范围的ASA浓度计算出来。由酶确定的ASA值也与使用Cucumber AAO.的瓣膜相媲美。这些多烯醇还表明了用于氧化氧化的抗氧化活性。年轻的水果提取物显示了大约两个月的抗氧化活性。这些结果表明，日本梨的年轻水果的提取物已用于用于脂肪食品的天然氧化剂。

项目成果

藤田修二: "Distribution of ascorbate oxidase in citrus fruits." Food Sci.Technol.,Int.2(3). 154-156 (1996)

Shuji Fujita：“柑橘类水果中抗坏血酸氧化酶的分布。”食品科学技术，Int.2(3) (1996)。

藤田 修二: "Distribution of ascorbate oxidase in citrus fruits." Food Sci.Technol.,Int.Tokyo. 2(3). 154-156 (1996)

Shuji Fujita：“柑橘类水果中抗坏血酸氧化酶的分布”，食品科学技术，东京，154-156。

N.Saari, S.Fujita, S.Yamaguchi and T.Tono: "Distribution of ascorbate oxidase in citrus fruit" Food Sci.Techno., Int.Tokyo. 2(3). 154-156 (1996)

N.Saari、S.Fujita、S.Yamaguchi 和 T.Tono：“柑橘类水果中抗坏血酸氧化酶的分布”食品科学技术，Int.Tokyo。