Studies on the functional substances from young fruits of Satsumamandarin and Japanese pear.

萨摩柑橘和梨幼果功能物质的研究。

• 批准号: 08660037
• 负责人: FUJITA Shuji
• 金额: $ 1.41万
• 依托单位: Saga university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660037/
• 关键词: Citrus fruit; Ascorbic acid; Ascorbic acid analysis; Antioxidative substance; Polyphenol; Japanese pear; Biofunctional substance; Polyphenol oxidase; ニホンナシ; 抗酸化能; ポリフェノール類

Twenty cultivars of citrus fruits were harvested two stages of development : young fruit (weight about 2.5 gper fruit) and fully ripe fruit of which the ascorbic acid oxidase (AAO) activities were determined. Among the different cultivars, the specific activities of AAO in the young fruit ranged from 47 to 2120 units per mg protein, while fully ripe fruits had AAO ranging from 18 to 98 unit per mg protein. Significant differences were also found between tissue types in the AAO activity with the higest activity in albedo (64-70%), followed by the flavedo (26-34%) and the pulp (2-3%).AAO obtained from the young fruits of Satsuma mandarin was utilized for the enzymatic determination of ascorbic acid (AsA) based on the difference spectral method as a possible new source of AAO,and the results was compared to cucumber AAO.A linear relationship was observed between AAO activity calculated from the maximum peak height of the difference spectra and the AsA concentration in the ranges of 0-100 ug/ml. The values of AsA determined by the enzyme were also comparable to the valuse obtained using cucumber AAO.Thus, AAO from the young fruit could provide a cheaper source of the enzyme for AsA determination.The crude extract obtained from young fruits of Japanese pear had strong antioxidative activity for linoleic acid oxidation as well as BHA.The extract contained polyphinols such as chlorogenic acid and catechin. These polypenols also indicated antioxidative activity for Jinoleic acid oxidation. The young fruit extract showed antioxidative activity for about two month. These results invesitgated that the extract from young fruits of Japanese pear had been employed for a natural aitioxidant for fatty foods.

收获两个发育阶段的二十个柑橘类水果品种：幼果（每个果实重量约2.5克）和完全成熟的果实，测定其抗坏血酸氧化酶（AAO）活性。在不同品种中，幼果中AAO的比活性范围为47至2120单位/毫克蛋白质，而完全成熟的果实中AAO的比活性范围为18至98单位/毫克蛋白质。组织类型之间的 AAO 活性也存在显着差异，反照率活性最高 (64-70%)，其次是黄皮 (26-34%) 和果肉 (2-3%)。 AAO 从幼苗中获得作为AAO的一个可能的新来源，利用差异光谱法对萨摩柑橘果实中的抗坏血酸（AsA）进行了酶法测定，并将结果与​​黄瓜AAO进行了比较。从差异光谱的最大峰高计算出的AAO活性与0-100ug/ml范围内的AsA浓度之间观察到线性关系。该酶测定的 AsA 值也与使用黄瓜 AAO 获得的值相当。因此，来自幼果的 AAO 可以为 AsA 测定提供更便宜的酶来源。从日本梨幼果中获得的粗提物具有很强的抗氧化活性。亚油酸氧化和 BHA 具有抗氧化活性。提取物含有绿原酸和儿茶素等多酚类化合物。这些多酚还表现出对金油酸氧化的抗氧化活性。幼果提取物显示抗氧化活性约两个月。这些结果表明，日本梨幼果的提取物已被用作脂肪食品的天然抗氧化剂。

项目成果

藤田修二: "Distribution of ascorbate oxidase in citrus fruits." Food Sci.Technol.,Int.2(3). 154-156 (1996)

Shuji Fujita：“柑橘类水果中抗坏血酸氧化酶的分布。”食品科学技术，Int.2(3) (1996)。

藤田 修二: "Distribution of ascorbate oxidase in citrus fruits." Food Sci.Technol.,Int.Tokyo. 2(3). 154-156 (1996)

Shuji Fujita：“柑橘类水果中抗坏血酸氧化酶的分布”，食品科学技术，东京，154-156。

N.Saari, S.Fujita, S.Yamaguchi and T.Tono: "Distribution of ascorbate oxidase in citrus fruit" Food Sci.Techno., Int.Tokyo. 2(3). 154-156 (1996)

N.Saari、S.Fujita、S.Yamaguchi 和 T.Tono：“柑橘类水果中抗坏血酸氧化酶的分布”食品科学技术，Int.Tokyo。