Studies on the affecting factors of the storage stability of shredded vegetables.

蔬菜丝贮藏稳定性影响因素研究

• 批准号: 03660029
• 负责人: FUJITA Shuji
• 金额: $ 1.54万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660029/
• 关键词: Shredded vegetables; Japanese raddish; Polyphenol oxidase; Polyphenol oxidase inhibitor; Enzymatic browning; Prevention of enzymatic browing; 褐変防止; 褐変阻害; 鮮度保持態; ポリフェノ-ル酸化酵素; ポリフェノ-ル酸化酵素インヒビタ-

Prevention of enzymatic browning in fruits and vegetables was investigated. Polyphenol oxidase inhibitors was founud in various vegetables, and the inhibiting effect of Japanese raddish extract was highest. The inhibitor was purified from the Japanese raddish extract by heat-denaturation of protein, fractionation of ethyl acetate, and sephadex G-25 gel filtration. The inhibitor fraction was further fractionated using hydrophobic chromatography TLC and HPLC.The purified inhibitor (JRI) showed absorption peak at 270 nm, and was positive for FeCl_3 but negative for ninhydrin. JRI was eluted after glucose on SephadexG-25. These results indicate that JRI is a low molecular weight polyphenolic compound.JRI inhibited polyphenol oxidase competitively. JRI also inhibited enzymatic browning finding at cutsurface of lettuce and edible burdock.These results indicate that JRI is useful for keeping the storage stability of shredded vegetables, such as sheredded lettuce and cabbage.

研究了在水果和蔬菜中预防酶褐变。多酚氧化酶抑制剂在各种蔬菜中都是fuunud，日本辐射提取物的抑制作用最高。通过蛋白质的热增饱和，乙酸乙酯和Sephadex G-25凝胶过滤从日本的辐射提取物中纯化抑制剂。使用疏水色谱TLC和HPLC进一步分级抑制剂级分。纯化的抑制剂（JRI）在270 nm处显示出吸收峰，而FECL_3则呈阳性，但忍者含量为阴性。 JRI在Sephadexg-25上葡萄糖后洗脱。这些结果表明，JRI是一种低分子量多酚化合物。JRI竞争性地抑制多酚氧化酶。 JRI还抑制了生菜和食用牛d的切割表面上的酶褐变发现。这些结果表明，JRI有助于保持切碎蔬菜的储存稳定性，例如杂草的生菜和卷心菜。

项目成果

藤田修二: "Spectrophotometric determination of Phlorog〓ucinol oxidase activity based on difference spectra" Bull.Fac.Agr.,Saga Univ.74. 81-86 (1993)

Shuji Fujita：“基于差异光谱的间苯三酚氧化酶活性的分光光度测定”Bull.Fac.Agr.，Saga Univ.74 (1993)。

藤田修二: "Spectrophotonetric determination of phlorogrucinol oxidase activity based on difference spectra." Bull.Fac.Agr.,Saga Univ. 74. 81-86 (1993)

Shuji Fujita：“基于差异光谱的间苯三酚氧化酶活性的分光光度法测定。”Bull.Fac.Agr.，佐贺大学 74. 81-86 (1993)

FUJITA,Shuji: "Spectrophotometric determination of phloroglucinol oxidase activity based on difference spectra" Bull.Fac.Agr., Saga Univ.No74. 81-86 (1993)

FUJITA，Shuji：“基于差异光谱的间苯三酚氧化酶活性的分光光度测定”Bull.Fac.Agr.，佐贺大学No74。