Development of Lactic Acid Fermentation Process Accompanied with Selective Product Separation

伴随选择性产物分离的乳酸发酵工艺的开发

基本信息

批准号：
08555195
负责人：
HANO Tadashi
金额：
$ 5.44万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The fermentative production process of L-lactic and as a raw material of biodegradable polymer was developed by employing Lactobacillus rhamnosus and Rhizopus oryzae and the following results were obtained concerning the fermentation and products separation.1) In the extractive fermentation with Lactobacillus rhamnosus, it became possible to remove completely the dissolved extractant which is toxic for microbes by using a cleaning column packed with cation exchange resin. The product inhibition, which severely retarded the fermentation under the initial glucose concentration of 100g/L, was suppressed with the present extractive fermentation system and fermentation proceeded satisfactorily.2) In the electrodialysis fermentation with Lactobacillus rhamnosus, a continuous fermentation method utilizing the permeation of water was found to give a stable production of lactic acid for long term. By using a glucose sensor in a fermentor, it was possible to keep the concentration of glucose at low level and to increase the yield of lactic acid.3) In the fermentation with Rhizopus oryzae, the addition of activated carbon to the fermentation broth was very effective in increasing the productivity and yield and obtaining clean broth which simplified the downstream processing.4) The lactic acid production systems investigated in this project were evaluated. It was concluded that an extractive fermentation with Lactobacillus rhamnosus was suitable for a small-scale batch operation whereas an electrodialysis fermentation with the same bacteria was for a small-scale continuous operation. Rhizopus oryzae was suitable for a large-scale fermentation.

开发了利用鼠李糖乳杆菌和米根霉发酵生产L-乳酸作为生物可降解聚合物原料的工艺，并在发酵和产物分离方面得到了以下结果。 1）在鼠李糖乳杆菌提取发酵中，得到了以下结果：通过使用填充有阳离子交换树脂的清洗柱，可以完全去除对微生物有毒的溶解萃取剂。在初始葡萄糖浓度为 100g/L 的情况下，产物抑制严重阻碍了发酵，而本提取发酵系统抑制了产物抑制，并且发酵进行得令人满意。 2) 在鼠李糖乳杆菌的电渗析发酵中，一种利用渗透的连续发酵方法。人们发现水可以长期稳定地产生乳酸。通过在发酵罐中使用葡萄糖传感器，可以将葡萄糖浓度保持在较低水平，并提高乳酸产量。3）在米根霉发酵中，发酵液中添加的活性炭非常有效。有效提高生产率和产量并获得清洁的肉汤，从而简化了下游加工。4）对本项目研究的乳酸生产系统进行了评估。结论是，鼠李糖乳杆菌提取发酵适合小规模批量操作，而相同细菌的电渗析发酵适合小规模连续操作。米根霉适合大规模发酵。